Perforin-IN-2
Based on 1 publication(s) in Google Scholar
Perforin-IN-2 (Compound 1) is the inhibitor for perforin that inhibits perforin-mediated cell lysis, thereby reducing graft rejection during allogeneic bone marrow/stem cell transplantation.
For research use only. We do not sell to patients.
- Purity: 99.43%
- CAS No.: 1567836-70-7
- Formula: C24H18N4O5S2
- Molecular Weight:506.55
-
Storage:Powder -20°C, 3 years , 4°C, 2 years ; In solvent -80°C, 6 months , -20°C, 1 month
-
Biological Activity
-
Chemical Information
-
Solvent & Solubility
- Purity & Documentation
- References
-
Help & FAQs
-
Immunology/Inflammation Compound Library
HY-L007
-
Anti-Cancer Compound Library
HY-L025
-
Anti-Blood Cancer Compound Library
HY-L079
-
Autoimmune Disease Compound Library
HY-L156
-
Anti-Hematopathy Compound Library
HY-L171
-
Bioactive Compound Library Max
HY-L181
-
MCE Bioactive Compound Library
HY-L001V
-
Bioactive Compound Library
HY-L001
-
High-Throughput Bioactive Compound Library
HY-L205
Publications Citing Use of MedChemExpress (MCE) Perforin-IN-2
More
Biological Activity
Perforin-IN-2 (10 μM, 24 h) inhibits the lytic activity of perforin against Jurkat T leukemia cell (IC50=6.65 μM), inhibits the killing effect of KHYG-1 NK cell (effector cells) on K562 cell (IC50=5.37 μM)[1].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
-
Cell Line:Jurkat T, K562
-
Concentration:10 μM
-
Incubation Time:24 h
-
Result:Improved the cell viability decreased by recombinant perforin and KHYG-1 NK cell.
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
-
Animal Model:C57BL/6 mouse models[1]
-
Dosage:80-160 mg/kg
-
Administration:ip, single dose
-
Result:Increased the survival rate of bone marrow cells in the spleen and peripheral blood.
Chemical Information
-
CAS No. 1567836-70-7
-
Appearance Solid
-
Molecular Weight 506.55
-
Formula C24H18N4O5S2
-
Color Light yellow to yellow
-
SMILES
O=C1C2=C(CN1C)C=C(C3=CC=C(C4=CN=CC(NS(=O)(C5=C([N+]([O-])=O)C=CC=C5)=O)=C4)S3)C=C2
-
Shipping
Room temperature in continental US; may vary elsewhere.
-
Storage
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month
Publications (1)
-
Journal Impact Factor
-
Most Recent
-
bioRxiv
2025 Nov 20:2025.11.19.689204. PMID: 41332750
Solvent & Solubility
DMSO : 33.33 mg/mL (65.80 mM; ultrasonic and warming and heat to 60°C; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Select the appropriate dissolution method based on your experimental animal and administration route.
- For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
- To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
- The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.
Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 2.5 mg/mL (4.94 mM); Clear solution
This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: ≥ 2.5 mg/mL (4.94 mM); Clear solution
This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
Please enter the basic information of animal experiments:
-
-
-
-
Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
-
%DMSO +
Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
-
%+
-
+%Tween-80 + +
-
%Saline +
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Working solution concentration: 0.22 mg/mL
Method for preparing stock solution: mg drug dissolved in μL DMSO. Stock solution concentration: mg/mL.
1. Take μL DMSO stock solution;
2. Add μL .
μL , mix evenly;
3. Then add μL Tween 80, mix evenly;
4. Then add μL
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
Purity & Documentation
-
Data Sheet (276 KB)
-
SDS (251 KB)
- English - EN (251 KB)
- Français - FR (251 KB)
- Deutsch - DE (251 KB)
- Norwegian - NO (251 KB)
- Español - ES (251 KB)
- Swedish - SV (251 KB)
- Italian - IT (251 KB)
- Portuguese - PT (251 KB)
-
Handling Instructions (2659 KB)
References
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| DMSO | 1 mM | 1.9741 mL | 9.8707 mL | 19.7414 mL | 49.3535 mL |
| 5 mM | 0.3948 mL | 1.9741 mL | 3.9483 mL | 9.8707 mL | |
| 10 mM | 0.1974 mL | 0.9871 mL | 1.9741 mL | 4.9353 mL | |
| 15 mM | 0.1316 mL | 0.6580 mL | 1.3161 mL | 3.2902 mL | |
| 20 mM | 0.0987 mL | 0.4935 mL | 0.9871 mL | 2.4677 mL | |
| 25 mM | 0.0790 mL | 0.3948 mL | 0.7897 mL | 1.9741 mL | |
| 30 mM | 0.0658 mL | 0.3290 mL | 0.6580 mL | 1.6451 mL | |
| 40 mM | 0.0494 mL | 0.2468 mL | 0.4935 mL | 1.2338 mL | |
| 50 mM | 0.0395 mL | 0.1974 mL | 0.3948 mL | 0.9871 mL | |
| 60 mM | 0.0329 mL | 0.1645 mL | 0.3290 mL | 0.8226 mL |
Powered by Bioz