country

Change Language

EN - English
FR - Français
DE - Deutsch
ES - Español
JA - 日本語
KO - 한국어

Country or Region:

United States

Product Name	Size / Unit Price	Qty

Total: USD 0.00 Checkout

Cat. No.: HY-W989139

Copy Link Print Information

Perforin-IN-3

Perforin-IN-3 is a specific perforin inhibitor that blocks perforin-mediated lytic pore formation and subsequent cell lysis. Perforin-IN-3 potently inhibits the killing effect of NK cells on target cells such as leukemia cells, without interfering with FasL- or TRAIL-mediated cell death pathways. Perforin-IN-3 has no effect on pneumolysin and can be used to study autoimmune diseases, allograft rejection, graft-versus-host disease, and familial hemophagocytic lymphohistiocytosis.

For research use only. We do not sell to patients.

CAS No.: 178970-88-2
Formula: C₁₄H₁₁NO₃S
Molecular Weight:273.31

Storage:

Please store the product under the recommended conditions in the Certificate of Analysis.

Perforin-IN-3 Chemical Structure

CAS No.: 178970-88-2

Size

Price

Stock

Quantity

Amount: USD 0.00

* Please select Quantity before adding items.

Top Publications Citing

Use of MCE Products

Fluorescent Dye

Strong Fluorescence Signal / Multi-Disciplinary Applications / High Fluorescence Specificity

Biological Activity
- Description
- Cellular Effect
- In Vitro
Chemical Information
Purity & Documentation
References
Calculators
Help & FAQs
Classifications
Bioz

Biological Activity

Description

Cellular Effect

Cell Line	Type	Value	Description	References
Jurkat	IC₅₀	2.7 μM Compound: 1	Inhibition of recombinant perforin-mediated lysis of human Jurkat T cells by 51Cr release assay Inhibition of recombinant perforin-mediated lysis of human Jurkat T cells by 51Cr release assay	[PMID: 19007200]

In Vitro

Perforin-IN-3 (compound 1) inhibits perforin-mediated lysis of Jurkat T lymphoma cells, with an IC₅₀ (4 h) of 2.7 μM^[1].
Perforin-IN-3 inhibits the K562 leukemia target cell killing rate mediated by KHYG-1 NK cells by 50%, and the survival rate of KHYG-1 NK cells remains 72% after 24 h of exposure^[1].
Perforin-IN-3 (20 μM; 30 min) does not inhibit Fas ligand- or TRAIL-mediated Jurkat T lymphoma cell death^[1].
Perforin-IN-3 inhibits primary human NK cell-mediated lysis of K562 leukemia target cells by over 70%^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Chemical Information

CAS No. 178970-88-2
Molecular Weight 273.31
Formula C₁₄H₁₁NO₃S
SMILES

C(=C\1/C2=C(C(=O)O1)C(=S)NC(C)=C2)\C3=CC=C(C)O3
Shipping

Room temperature in continental US; may vary elsewhere.
Storage

Please store the product under the recommended conditions in the Certificate of Analysis.

Purity & Documentation

Handling Instructions (2659 KB)

References

[1]. Lena G, et al. Dihydrofuro [3, 4-c] pyridinones as inhibitors of the cytolytic effects of the pore-forming glycoprotein perforin[J]. Journal of medicinal chemistry, 2008, 51(23): 7614-7624.

Calculators

Molarity Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass Mass

Concentration Concentration

Volume Volume

Molecular Weight Molecular Weight

Dilution Calculator

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

Concentration _(start) Concentration _(start)

Volume _(start) Volume _(start)

Concentration _(final) Concentration _(final)

Volume _(final) Volume _(final)

Help & FAQs

How should lyophilized recombinant proteins be reconstituted and stored?

1. Before opening the cap, centrifuge the vial at 13000 rpm for 20-30 seconds. This step will ensure that any lyophilized powder that may have adhered to the cap or walls is collected at the bottom of the vial, minimizing the risk of product loss.

Keywords

MOQ

Minimum order quantity

100 mg

Get Quote In-stock

Other size

Get Quote