2. Immunology/Inflammation Apoptosis
  3. CD44 Apoptosis
  4. PF-03475952

PF-03475952 is a fully human IgG2 monoclonal antibody targeting CD44. PF-03475952 binds an epitope in CD44’s constant exons, blocks CD44-hyaluronic acid interaction, reduces cell surface CD44, and does not cross-react with rodent CD44 or LYVE-1. PF-03475952 induces cancer cell apoptosis, inhibits LPS (HY-D1056)-induced leukocyte cytokine release and cancer metastasis, and reduces CD44 expression on circulating CD3+ lymphocytes in cynomolgus monkeys. PF-03475952 can be used for the research of rheumatoid arthritis and hepatocellular carcinoma.

For research use only. We do not sell to patients.

PF-03475952

PF-03475952 Chemical Structure

Size Stock
1 mg   Get quote  
5 mg   Get quote  
10 mg   Get quote  

* Please select Quantity before adding items.

This product is a controlled substance and not for sale in your territory.

PF-03475952 Related Antibodies

Top Publications Citing Use of Products

  • Biological Activity

  • Purity & Documentation

  • References

  • Customer Review

Description

PF-03475952 is a fully human IgG2 monoclonal antibody targeting CD44. PF-03475952 binds an epitope in CD44’s constant exons, blocks CD44-hyaluronic acid interaction, reduces cell surface CD44, and does not cross-react with rodent CD44 or LYVE-1. PF-03475952 induces cancer cell apoptosis, inhibits LPS (HY-D1056)-induced leukocyte cytokine release and cancer metastasis, and reduces CD44 expression on circulating CD3+ lymphocytes in cynomolgus monkeys. PF-03475952 can be used for the research of rheumatoid arthritis and hepatocellular carcinoma[1][2].

Species Reactivity

Human
In Vitro

PF-03475952 (0.0095-10000 ng/mL) binds potently and selectively to recombinant human and cynomolgus monkey CD44-Ig fusion proteins with EC50 values of 0.017 µg/mL and 0.015 µg/mL, respectively[1].
PF-03475952 (60 min) potently inhibits the interaction between recombinant CD44-Ig and HA with an IC50 of 0.41 µg/mL[1].
PF-03475952 (24 h) induces dose-dependent loss of cell surface CD44 from human CD14+ monocytes (IC50 = 3.3 µg/mL), human CD19+ B cells (IC50 = 2.4 µg/mL), human CD3+ T cells (IC50 = 2.6 µg/mL), and cynomolgus monkey CD3+ T cells (IC50 = 1.1 µg/mL) in whole blood[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

PF-03475952 Related Antibodies
In Vivo

PF-03475952 (1-100 mg/kg; i.v.; single dose) reduces CD44 expression on cynomolgus monkey CD3+ lymphocytes in a dose-dependent manner[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model: Cynomolgus monkeys[1]
Dosage: 1; 10; 100 mg/kg
Administration: i.v.; single dose
Result: Reduced CD44 expression on CD3+ lymphocytes below 50% of baseline for 218 hours at 1 mg/kg.
Reduced CD44 expression on CD3+ lymphocytes below 50% of baseline for 373 hours at 10 mg/kg.
Reduced CD44 expression on CD3+ lymphocytes below 50% of baseline for >504 hours at 100 mg/kg.
Achieved approximately 50% reduction in cell surface CD44 at serum levels of approximately 0.17 µg/mL for 1 mg/kg and 10 mg/kg dose groups.
Restored CD44 levels to baseline by day 22 (504 hours) in 1 mg/kg and 10 mg/kg dose groups.
Gene ID

960  [NCBI]

Accession
Target

CD44
Application

ELISA, FACS, Functional assay
Conjugated

Unconjugated
Reconsititution

The product can be reconstituted/diluted with sterile PBS or saline.
Formulation

Please refer to the lot-specific COA for specific buffer information.
Storage

Please store the product under the recommended conditions in the Certificate of Analysis.
Purity & Documentation
References
  • No file chosen (Maximum size is: 1024 Kb)
  • If you have published this work, please enter the PubMed ID.
  • Your name will appear on the site.

PF-03475952 Related Classifications

  • Molarity Calculator

  • Dilution Calculator

The molarity calculator equation

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass   Concentration   Volume   Molecular Weight *
= × ×

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
× = ×
C1   V1   C2   V2
Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

PF-03475952PF03475952PF 03475952CD44ApoptosisIn (Lu)-related protein p80Pgp-1/Ly-24ECMRIIIHUTCH-1cancer cell apoptosisCD3+ lymphocytesperipheral blood mononuclear cellsFc gamma receptorshyaluronic acidrheumatoid arthritisLYVE-1cynomolgus monkeyhepatocellular carcinomaInhibitorinhibitorinhibit

Your Recently Viewed Products:

Inquiry Online

Your information is safe with us. * Required Fields.

Product Name

  

Requested Quantity *

Applicant Name *

 

Salutation

Email Address *

 

Phone Number *

Department

 

Organization Name *

City

State

Country or Region *

      

Remarks

Bulk Inquiry

Inquiry Information

Product Name:
PF-03475952
Cat. No.:
HY-P992437
Quantity:
MCE Japan Authorized Agent:

Customer Review

Thank You for Your Feedback!

Request for HNMR Report

Please fill out this form to request the QC report. We will send it to your Email address shortly.

Your information is safe with us. * Required Fields.

Product Name

  

Lot #

Applicant Name *

 

Email Address *

Phone Number

 

Department *

Organization Name *

 

Country or Region *

Remarks

SAFETY DATA SHEET (SDS)

Request for HNMR Report

We have received your request and will respond to you as soon as possible.