Phaseoloideside B
Phaseoloideside B is an oleanane-type triterpenoid saponin found in Entada phaseoloides. Phaseoloideside B shows no cytotoxicity against cancer cells.
For research use only. We do not sell to patients.
- CAS No.: 1453187-71-7
- Formula: C65H105NO32
- Molecular Weight:1412.53
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Storage:
Please store the product under the recommended conditions in the Certificate of Analysis.
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
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Biological Activity
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Chemical Information
- Protocols
- Purity & Documentation
- References
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Help & FAQs
Biological Activity
Description
Cellular Effect
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Cell Line
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Type | Value | Description | References |
|---|---|---|---|---|
| HepG2 | IC50 |
>100 μM
|
Cytotoxicity against human HepG-2 cells assessed as reduction in cell viability incubated for 48 hrs by MTT assay.
Cytotoxicity against human HepG-2 cells assessed as reduction in cell viability incubated for 48 hrs by MTT assay.
|
hlca.201200491 |
| SW480 | IC50 |
>100 μM
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Cytotoxicity against human SW480 cells assessed as reduction in cell viability incubated for 48 hrs by MTT assay.
Cytotoxicity against human SW480 cells assessed as reduction in cell viability incubated for 48 hrs by MTT assay.
|
hlca.201200491 |
| ECa-109 cell line | IC50 |
>100 μM
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Cytotoxicity against human Eca-109 cells assessed as reduction in cell viability incubated for 48 hrs by MTT assay.
Cytotoxicity against human Eca-109 cells assessed as reduction in cell viability incubated for 48 hrs by MTT assay.
|
hlca.201200491 |
| HeLa | IC50 |
>100 μM
|
Cytotoxicity against human HeLa cells assessed as reduction in cell viability incubated for 48 hrs by MTT assay.
Cytotoxicity against human HeLa cells assessed as reduction in cell viability incubated for 48 hrs by MTT assay.
|
hlca.201200491 |
In Vitro
Phaseoloideside B (48 h) shows no in vitro cytotoxicity against HepG-2, SW480, Eca-109, and HeLa cell lines with IC50 values greater than 100 μM[1].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only. Further protocols information, click here.
Chemical Information
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CAS No. 1453187-71-7
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Molecular Weight 1412.53
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Formula C65H105NO32
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SMILES
C(O[C@H]1[C@H](O[C@H]2[C@H](O)[C@@H](O)[C@H](O)CO2)[C@@H](O)[C@H](O)[C@@H](CO)O1)(=O)[C@]34[C@](C=5[C@@](C)([C@@H](O)[C@H]3O)[C@@]6(C)[C@](CC5)([C@]7(C)[C@@](CC6)(C(C)(C)[C@@H](O[C@H]8[C@H](NC(C)=O)[C@@H](O)[C@H](O[C@@H]9O[C@H](CO)[C@@H](O)[C@H](O)[C@H]9O)[C@@H](CO[C@H]%10[C@H](O)[C@@H](O[C@H]%11[C@H](O)[C@@H](O)[C@H](O)CO%11)[C@@H](O)CO%10)O8)CC7)[H])[H])(CC(C)(C)CC4)[H]
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Structure Classification
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
Please store the product under the recommended conditions in the Certificate of Analysis.
Protocols
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Cell Cytotoxicity Assay
Cytotoxicity assays are usually based on the assessment of cell membrane damage, which can also be indirectly detected by measuring cell viability. Detection methods include MTT assay, CKK-8 assay, LDH assay and ATP assay, etc.
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Mammalian live/dead viability and cytotoxicity staining
Live/dead viability and cytotoxicity staining assays are based on the simultaneous detection of intracellular esterase activity in metabolically active (viable) cells and membrane integrity loss in non-viable cells. In commonly used dual-staining approaches, membrane-permeant fluorogenic substrates are converted by intracellular esterases into fluorescent products in live cells, while impermeant DNA-binding dyes selectively enter cells with compromised plasma membranes and label nucleic acids in dead or dying cells, enabling discrimination between viable and non-viable populations by fluorescence microscopy or flow cytometry.
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Cell Viability Determination by MTT Colorimetric Assay
The following protocol uses the MTT colorimetric assay as a classic literature-established method for assessing cell viability/metabolic activity in cultured mammalian cells. MTT[3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide] is reduced by metabolically active cells to a colored formazan product; the amount of formazan is quantified spectrophotometrically and provides an indirect measure of metabolically active viable cells. Importantly, MTT reduction reflects cellular oxidoreductase/metabolic activity rather than an absolute direct count of living cells, so changes in cellular metabolism can alter the signal independently of cell number.
Purity & Documentation
References
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Phaseoloideside B Related Classifications
HY-N21726 Related Classifications
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