PHGDH-IN-5
Based on 1 Customer Validation
PHGDH-IN-5 (Compound B12) is a covalen inhibitor of PHGDH with an IC50 value of 0.29 μM. PHGDH-IN-5 can inhibit cell proliferation in cancer cell lines overexpressing PHGDH. PHGDH-IN-5 can reduce the production of serine derived from glucose in MDA-MB-468 cells.
For research use only. We do not sell to patients.
- Purity: 98.12%
- CAS No.: 17402-86-7
- Formula: C8H5NO4S
- Molecular Weight:211.19
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Storage:Powder -20°C, 3 years , 4°C, 2 years ; In solvent -80°C, 6 months , -20°C, 1 month
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
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Biological Activity
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Chemical Information
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Solvent & Solubility
- Purity & Documentation
- References
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Help & FAQs
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Metabolism/Protease Compound Library
HY-L012
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Anti-Cancer Compound Library
HY-L025
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Anti-Cancer Metabolism Compound Library
HY-L083
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Metabolic Enzyme Compound Library
HY-L146
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Bioactive Compound Library Max
HY-L181
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MCE Bioactive Compound Library
HY-L001V
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Bioactive Compound Library
HY-L001
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High-Throughput Bioactive Compound Library
HY-L205
Biological Activity
IC50: 0.29 μM (PHGDH)[1].
PHGDH-IN-5 can inhibit the proliferation of MDA-MB-468, NCI-H1975, HT1080, and PC9 cells, with IC50 values ranging from 9.93 μM to 21.2 μM[1].
PHGDH-IN-5 (10-30 μM) reduces the production of M+3-serine derived from 13C6-glucose in MDA-MB-468 cells[1].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
Chemical Information
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CAS No. 17402-86-7
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Appearance Solid
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Molecular Weight 211.19
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Formula C8H5NO4S
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Color Off-white to light yellow
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SMILES
O=S1(C2=CC=CC([N+]([O-])=O)=C2C=C1)=O
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month
Solvent & Solubility
DMSO : 100 mg/mL (473.51 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Select the appropriate dissolution method based on your experimental animal and administration route.
- For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
- To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
- The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.
Please enter the basic information of animal experiments:
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Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
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%DMSO +
Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
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%+
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+%Tween-80 + +
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%Saline +
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Working solution concentration: 0.22 mg/mL
Method for preparing stock solution: mg drug dissolved in μL DMSO. Stock solution concentration: mg/mL.
1. Take μL DMSO stock solution;
2. Add μL .
μL , mix evenly;
3. Then add μL Tween 80, mix evenly;
4. Then add μL
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
Purity & Documentation
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Data Sheet (278 KB)
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SDS (251 KB)
- English - EN (251 KB)
- Français - FR (251 KB)
- Deutsch - DE (251 KB)
- Norwegian - NO (251 KB)
- Español - ES (251 KB)
- Swedish - SV (251 KB)
- Italian - IT (251 KB)
- Korean - KR (251 KB)
- Portuguese - PT (251 KB)
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Handling Instructions (2659 KB)
References
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| DMSO | 1 mM | 4.7351 mL | 23.6754 mL | 47.3507 mL | 118.3768 mL |
| 5 mM | 0.9470 mL | 4.7351 mL | 9.4701 mL | 23.6754 mL | |
| 10 mM | 0.4735 mL | 2.3675 mL | 4.7351 mL | 11.8377 mL | |
| 15 mM | 0.3157 mL | 1.5784 mL | 3.1567 mL | 7.8918 mL | |
| 20 mM | 0.2368 mL | 1.1838 mL | 2.3675 mL | 5.9188 mL | |
| 25 mM | 0.1894 mL | 0.9470 mL | 1.8940 mL | 4.7351 mL | |
| 30 mM | 0.1578 mL | 0.7892 mL | 1.5784 mL | 3.9459 mL | |
| 40 mM | 0.1184 mL | 0.5919 mL | 1.1838 mL | 2.9594 mL | |
| 50 mM | 0.0947 mL | 0.4735 mL | 0.9470 mL | 2.3675 mL | |
| 60 mM | 0.0789 mL | 0.3946 mL | 0.7892 mL | 1.9729 mL | |
| 80 mM | 0.0592 mL | 0.2959 mL | 0.5919 mL | 1.4797 mL | |
| 100 mM | 0.0474 mL | 0.2368 mL | 0.4735 mL | 1.1838 mL |
PHGDH-IN-5 Related Classifications
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