PM534

PM534 (0.625-5 μM) inhibits tubulin assembly activity in a concentration-dependent manner in a cell-free system^[1]. PM534 (1-2 nM; 24 h) induces complete depolymerization of the microtubule cytoskeleton and disrupts mitotic spindle formation in A549 cells^[1].
PM534 (0.06-240 nM; 72 h) inhibits cell proliferation in A549 (IC₅₀ = 1.5 nM), Calu-6, NCI-H23, NCI-H460, A2780 (IC₅₀ = 1.4 nM), A2780AD (IC₅₀ = 3.2 nM), HeLa (IC₅₀ = 1.6 nM), and HeLa βIII (IC₅₀ = 1.3 nM) cells, with a GI₅₀ value of 2.2 nM^[1].
PM534 (0.06-240 nM; 72 h) inhibits the growth of BT-474 (GI₅₀ = 2.3 nM), HCC1937 (GI₅₀ = 1.8 nM), HCC1954 (GI₅₀ = 22.4 nM), MCF7 (GI₅₀ = 2.1 nM), MDA-MB-231 (GI₅₀ = 2.2 nM), MDA-MB-436 (GI₅₀ = 2.6 nM), A2780 (GI₅₀ = 1.1 nM), ES-2 (GI₅₀ = 2.5 nM), IGROV-1 (GI₅₀ = 2.8 nM), SK-OV-3 (GI₅₀ = 2.5 nM), 22Rv1 (GI₅₀ = 2.1 nM), LN-Cap (GI₅₀ = 3.0 nM), PC-3 (GI₅₀ = 1.7 nM), and VCaP (GI₅₀ = 2.7 nM) cells^[2].
PM534 (10 nM; 1-24 h) induces irreversible anti-proliferative effects in MDA-MB-231 and MDA-MB-436 cells^[2].
PM534 (10-1000 nM; 2 h) competitively binds to the colchicine site and displaces EBI in cell lysates^[2].
PM534 (10 nM; 24 h) induces G2-M phase cell cycle arrest, upregulates Cyclin B1 and pH3 expression, and causes multinucleation in MDA-MB-231, A2780, and 22Rv1 cells^[2].
PM534 (0.5-10 nM; 30 min) inhibits cell adhesion activity in HUVEC cells (IC₅₀ = 2.3 nM)^[2].
PM534 (0.6-10 nM; 24 h) inhibits cell migration in HUVEC cells^[2].
PM534 (0.5-10 nM; 24 h) inhibits cell invasion, disrupts capillary-like tube formation, and disrupts established networks in HUVEC cells^[2].
PM534 (0.06-240 nM; 72 h) exhibits activity in overcoming drug resistance and inhibiting cell growth in LoVo (GI₅₀ = 1.9 nM), LoVo-DOX (GI₅₀ = 5.8 nM), HeLa wt (GI₅₀ = 1.1 nM), and HeLa βIII (GI₅₀ = 1 nM) cells^[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.