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Cat. No.: HY-149679

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PROTAC eDHFR Degrader-2

PROTAC eDHFR Degrader-2 is a PROTAC degrader targeting eDHFR. PROTAC eDHFR Degrader-2 promotes the formation of a ternary complex between eDHFR-tagged proteins and the E3 ligase Cereblon, driving ubiquitin-proteasome-mediated degradation. PROTAC eDHFR Degrader-2 downregulates eDHFR-YFP in lymphoid cells and embryonic kidney cells. PROTAC eDHFR Degrader-2 can be used in studies related to metastatic ovarian cancer.
(Pink: eDHFR ligand (HY-W587663); Blue: Cereblon ligand (HY-41547); Black: linker).

For research use only. We do not sell to patients.

CAS No.: 2849442-91-5
Formula: C₃₄H₄₀N₈O₉
Molecular Weight:704.73

Storage:

Please store the product under the recommended conditions in the Certificate of Analysis.

Data Sheet (268 KB)

PROTAC eDHFR Degrader-2 Chemical Structure

CAS No.: 2849442-91-5

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Fluorescent Dye

Strong Fluorescence Signal / Multi-Disciplinary Applications / High Fluorescence Specificity

Biological Activity
- Description
- IC₅₀ & Target
- In Vitro
Chemical Information
Purity & Documentation
References
Calculators
Help & FAQs
Classifications
Bioz

Biological Activity

Description

PROTAC eDHFR Degrader-2 is a PROTAC degrader targeting eDHFR. PROTAC eDHFR Degrader-2 promotes the formation of a ternary complex between eDHFR-tagged proteins and the E3 ligase Cereblon, driving ubiquitin-proteasome-mediated degradation. PROTAC eDHFR Degrader-2 downregulates eDHFR-YFP in lymphoid cells and embryonic kidney cells. PROTAC eDHFR Degrader-2 can be used in studies related to metastatic ovarian cancer^[1]. (Pink: eDHFR ligand (HY-W587663); Blue: Cereblon ligand (HY-41547); Black: linker).

IC₅₀ & Target

eDHFR^[1]

All PROTACs Isoforms :

In Vitro

PROTAC eDHFR Degrader-2 (compound 7b) (2 nM-100 μM; 4-24 h) potently degrades eDHFR-YFP in JURKAT-eDHFR-YFP cells, achieving nearly complete degradation at a concentration of 100 nM within 4 h of incubation^[1].
PROTAC eDHFR Degrader-2 (2 nM-100 μM; 24 h) effectively degrades eDHFR-YFP in HEK293T-eDHFR-YFP cells^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Western Blot Analysis^[1]

Cell Line:

HEK293T-eDHFR-YFP cells (HEK293T cells stably expressing eDHFR-YFP-T2A-firefly luciferase)
Concentration:

2 nM, 25 nM, 100 nM, 25 μM, 100 μM
Incubation Time:

24 h
Result:

Induced effective degradation of eDHFR-YFP at concentrations ranging from 2 nM to 100 nM, with the most robust degradation observed at 25 nM and 100 nM after 24 h.

Chemical Information

CAS No. 2849442-91-5
Molecular Weight 704.73
Formula C₃₄H₄₀N₈O₉
SMILES

NC1=C(CC2=CC(OC)=C(OCCCC(NCCOCCNC3=C(C(N(C4C(NC(CC4)=O)=O)C5=O)=O)C5=CC=C3)=O)C(OC)=C2)C=NC(N)=N1
Shipping

Room temperature in continental US; may vary elsewhere.
Storage

Please store the product under the recommended conditions in the Certificate of Analysis.

Purity & Documentation

Select Batch:

Purity: 99.98%

Assay: 99.98%

ee.: 99.98%

Data Sheet (268 KB)
COA (2659 KB)
Elemental Analysis Report (2659 KB)
HNMR (2659 KB)
CNMR (2659 KB)
RP-HPLC (2659 KB)
NP-HPLC (2659 KB)
MS (2659 KB)
LCMS (2659 KB)
IR (2659 KB)
GC (2659 KB)
OR (2659 KB)
SDS-PAGE (2659 KB)
SEC-HPLC (2659 KB)
Other Report (2659 KB)
Handling Instructions (2659 KB)

References

[1]. Etersque JM, et al. Regulation of eDHFR-tagged proteins with trimethoprim PROTACs. Nature communications. 2023 Nov 03;14(1):7071. [Content Brief]

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Help & FAQs

How should lyophilized recombinant proteins be reconstituted and stored?

1. Before opening the cap, centrifuge the vial at 13000 rpm for 20-30 seconds. This step will ensure that any lyophilized powder that may have adhered to the cap or walls is collected at the bottom of the vial, minimizing the risk of product loss.

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100 mg

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Biological Activity
- Description
- IC₅₀ & Target
- In Vitro
Chemical Information
Purity & Documentation
References

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PROTAC eDHFR Degrader-2

All PROTACs Isoforms

Biological Activity

Chemical Information

Purity & Documentation

References

Calculators

Help & FAQs

PROTAC eDHFR Degrader-2 Related Classifications

HY-149679 Related Classifications