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Cat. No.: HY-174443

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PROTAC sEH degrader-2

PROTAC sEH degrader-2 is a soluble epoxide hydrolase (sEH) PROTAC degrader, with pIC₅₀ values of 8.37 and 7.12 against hsEH-H and msEH-H, respectively. PROTAC sEH degrader-2 induces targeted degradation of sEH via the ubiquitin-proteasome system, thereby completely blocking its dual functions as an epoxide hydrolase and a lipid phosphatase by bridging the short-branch exit of sEH with the CRBN E3 ubiquitin ligase. PROTAC sEH degrader-2 can be used in the research of neuroinflammation and Alzheimer's disease.
(Pink: sEH ligand (HY-174225); Blue: Cereblon ligand (HY-103597); Black: linker).

For research use only. We do not sell to patients.

Formula: C₅₄H₅₇F₄N₉O₁₃S
Molecular Weight:1148.14

Storage:

Please store the product under the recommended conditions in the Certificate of Analysis.

Data Sheet (275 KB)

PROTAC sEH degrader-2 Chemical Structure

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Use of MCE Products

Strong Fluorescence Signal / Multi-Disciplinary Applications / High Fluorescence Specificity

Biological Activity
- Description
- IC₅₀ & Target
- In Vitro
Chemical Information
Purity & Documentation
References
Calculators
Help & FAQs
Classifications
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Biological Activity

Description

PROTAC sEH degrader-2 is a soluble epoxide hydrolase (sEH) PROTAC degrader, with pIC₅₀ values of 8.37 and 7.12 against hsEH-H and msEH-H, respectively. PROTAC sEH degrader-2 induces targeted degradation of sEH via the ubiquitin-proteasome system, thereby completely blocking its dual functions as an epoxide hydrolase and a lipid phosphatase by bridging the short-branch exit of sEH with the CRBN E3 ubiquitin ligase. PROTAC sEH degrader-2 can be used in the research of neuroinflammation and Alzheimer's disease^[1]. (Pink: sEH ligand (HY-174225); Blue: Cereblon ligand (HY-103597); Black: linker).

IC₅₀ & Target^[1]

hsEH-H

8.37 (pIC₅₀)

msEH-H

7.12 (pIC₅₀)

All PROTACs Isoforms :

In Vitro

PROTAC sEH degrader-2 (Compound 23) (3 nM-3 μM; 1-24 h) induces dose- and time-dependent degradation of sEH in HeLa^sEH-HiBiT cells^[1].
PROTAC sEH degrader-2 (3 μM; 3 h) significantly reduces the conversion of 14 (15)-, 11 (12)-, and 8 (9)-EpETrE into their corresponding DiHETrE metabolites in HeLa^sEH-HiBiT cell homogenates^[1].
PROTAC sEH degrader-2 (300 nM; 18 h) significantly reduces sEH expression levels in LPS (HY-D1056)- and IFNγ-activated human M1 macrophages, primary mouse hepatocytes, and precision-cut lung slices (PCLS)^[1].

PROTAC sEH degrader-2 (up to 50 μM; 72 h) does not induce significant cytotoxicity in HepG2 cells^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Immunofluorescence^[1]

Cell Line:

Human M1 macrophages (activated by LPS and IFNγ)
Concentration:

300 nM
Incubation Time:

18 h
Result:

Successfully triggered sEH degradation in primary human M1 macrophages.
Significantly downregulating sEH expression levels (approx. 10% residual expression) compared to the untreated group, while the inactive control 31 was ineffective.

Western Blot Analysis^[1]

Cell Line:

Primary murine hepatocytes
Concentration:

300 nM
Incubation Time:

18 h
Result:

Elicited significant degradation of sEH protein in primary murine hepatocytes (approx. 60% residual expression), though the degradation efficiency was lower than that observed in human macrophages.

Chemical Information

Molecular Weight 1148.14
Formula C₅₄H₅₇F₄N₉O₁₃S
SMILES

O=C(NCC1=C(C=C(C=C1)NS(=O)(CCCC2=CN(N=N2)CCOCCOCCOCCOCCNC(COC3=C(C(N(C4C(NC(CC4)=O)=O)C5=O)=O)C5=CC=C3)=O)=O)C(F)(F)F)C6=CC=C7N(C=CC7=C6)CC8=CC=CC(F)=C8
Shipping

Room temperature in continental US; may vary elsewhere.
Storage

Please store the product under the recommended conditions in the Certificate of Analysis.

Purity & Documentation

Select Batch:

Purity: 99.98%

Assay: 99.98%

ee.: 99.98%

Data Sheet (275 KB)
COA (2659 KB)
Elemental Analysis Report (2659 KB)
HNMR (2659 KB)
CNMR (2659 KB)
RP-HPLC (2659 KB)
NP-HPLC (2659 KB)
MS (2659 KB)
LCMS (2659 KB)
IR (2659 KB)
GC (2659 KB)
OR (2659 KB)
SDS-PAGE (2659 KB)
SEC-HPLC (2659 KB)
Other Report (2659 KB)
Handling Instructions (2659 KB)

References

[1]. Schönfeld J, et al. Structure-Based Design of PROTACS for the Degradation of Soluble Epoxide Hydrolase. Journal of medicinal chemistry. 2025 Jul 10;68(13):13728-13749. [Content Brief]

Calculators

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Help & FAQs

How should lyophilized recombinant proteins be reconstituted and stored?

1. Before opening the cap, centrifuge the vial at 13000 rpm for 20-30 seconds. This step will ensure that any lyophilized powder that may have adhered to the cap or walls is collected at the bottom of the vial, minimizing the risk of product loss.

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100 mg

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Biological Activity
- Description
- IC₅₀ & Target
- In Vitro
Chemical Information
Purity & Documentation
References

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PROTAC sEH degrader-2

All PROTACs Isoforms