2. Metabolic Enzyme/Protease
  3. Phosphatase
  4. PTP1B-IN-4

PTP1B-IN-4 is a non-competitive allosteric inhibitor of the protein tyrosine phosphatase PTP1B, with an IC50 of 8 μM. PTP1B-IN-4 is potentail for the research of obesity and diabetes.

For research use only. We do not sell to patients.

PTP1B-IN-4 Chemical Structure

PTP1B-IN-4 Chemical Structure

CAS No. : 765317-72-4

Size Price Stock Quantity
Solid + Solvent
10 mM * 1 mL in DMSO
ready for reconstitution
 USD 375 In-stock
Solution
10 mM * 1 mL in DMSO USD 375 In-stock
Solid
1 mg USD 109 In-stock
5 mg USD 230 In-stock
10 mg USD 380 In-stock
25 mg USD 800 In-stock
50 mg USD 1280 In-stock
100 mg USD 2100 In-stock
200 mg   Get quote  
500 mg   Get quote  

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Customer Review

Based on 1 publication(s) in Google Scholar

PTP1B-IN-4 Related Antibodies

Top Publications Citing Use of Products

  • Biological Activity

  • Purity & Documentation

  • References

  • Customer Review

Description

PTP1B-IN-4 is a non-competitive allosteric inhibitor of the protein tyrosine phosphatase PTP1B, with an IC50 of 8 μM. PTP1B-IN-4 is potentail for the research of obesity and diabetes[1][2].

IC50 & Target

IC50: 8 μM (PTP1B)[1]
In Vitro

PTP1B-IN-4 (250 μM; 1 hour) stimulates insulin receptor (IR) phosphorylation in CHO cells overexpressing human IR[1].
? PTP1B-IN-4 also induces phosphorylation of IRS-1 and Akt, proteins downstream of the insulin receptor[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

PTP1B-IN-4 Related Antibodies

Western Blot Analysis[1]

Cell Line: CHO cells
Concentration: 250 µM
Incubation Time: 1 hour
Result: Stimulated insulin receptor phosphorylation.
Molecular Weight

741.45

Formula

C26H19Br2N3O7S3
CAS No.
Appearance

Solid

Color

White to off-white

SMILES

O=S(C1=CC=C2C(C(C3=CC(Br)=C(O)C(Br)=C3)=O)=C(CC)OC2=C1)(NC4=CC=C(S(=O)(NC5=NC=CS5)=O)C=C4)=O
Shipping

Room temperature in continental US; may vary elsewhere.
Storage
Powder -20°C 3 years
In solvent -80°C 6 months
-20°C 1 month
Solvent & Solubility
In Vitro: 

DMSO : 100 mg/mL (134.87 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 1.3487 mL 6.7435 mL 13.4871 mL
5 mM 0.2697 mL 1.3487 mL 2.6974 mL
View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

  • Molarity Calculator

  • Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass
=
Concentration
×
Volume
×
Molecular Weight *

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start)

C1

×
Volume (start)

V1

=
Concentration (final)

C2

×
Volume (final)

V2

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

  • Protocol 1

    Add each solvent one by one:  10% DMSO    90% Corn Oil

    Solubility: ≥ 2.08 mg/mL (2.81 mM); Clear solution

    This protocol yields a clear solution of ≥ 2.08 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.

    Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.8 mg/mL) to 900 μL Corn oil, and mix evenly.

In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

g

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
%
DMSO +
% 
+
%
Tween-80 +
%
Saline
Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Calculation results:
Working solution concentration: mg/mL
Method for preparing stock solution: mg drug dissolved in μL  DMSO (Stock solution concentration: mg/mL).
The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).
Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.
 If the continuous dosing period exceeds half a month, please choose this protocol carefully.
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
Purity & Documentation

Purity: 99.81%

COA (250 KB) HNMR (291 KB) LCMS (265 KB)

Handling Instructions (2659 KB)
References

Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
DMSO 1 mM 1.3487 mL 6.7435 mL 13.4871 mL 33.7177 mL
5 mM 0.2697 mL 1.3487 mL 2.6974 mL 6.7435 mL
10 mM 0.1349 mL 0.6744 mL 1.3487 mL 3.3718 mL
15 mM 0.0899 mL 0.4496 mL 0.8991 mL 2.2478 mL
20 mM 0.0674 mL 0.3372 mL 0.6744 mL 1.6859 mL
25 mM 0.0539 mL 0.2697 mL 0.5395 mL 1.3487 mL
30 mM 0.0450 mL 0.2248 mL 0.4496 mL 1.1239 mL
40 mM 0.0337 mL 0.1686 mL 0.3372 mL 0.8429 mL
50 mM 0.0270 mL 0.1349 mL 0.2697 mL 0.6744 mL
60 mM 0.0225 mL 0.1124 mL 0.2248 mL 0.5620 mL
80 mM 0.0169 mL 0.0843 mL 0.1686 mL 0.4215 mL
100 mM 0.0135 mL 0.0674 mL 0.1349 mL 0.3372 mL
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PTP1B-IN-4 Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

PTP1B-IN-4765317-72-4PhosphataseproteintyrosinephosphatasePTP1BobesitydiabetesInhibitorinhibitorinhibit

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