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PTZ-343  (Synonyms: 3-(10′-Phenothiazinyl)propane-1-sulfonate sodium; SPTZ sodium)

Cat. No.: HY-112841 Purity: 99.97%
COA Handling Instructions

Molarity Calculator

PTZ-343 is a potent enhancer of Luminol (HY-15922). PTZ-343 greatly increases the light output of the peroxidase-catalyzed luminol chemiluminescent oxidation reaction (>80%).

For research use only. We do not sell to patients.

PTZ-343 Chemical Structure

PTZ-343 Chemical Structure

CAS No. : 101199-38-6

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Based on 1 publication(s) in Google Scholar

PTZ-343 Related Antibodies

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Description

PTZ-343 is a potent enhancer of Luminol (HY-15922). PTZ-343 greatly increases the light output of the peroxidase-catalyzed luminol chemiluminescent oxidation reaction (>80%)[1][2].

In Vitro

PTZ-343 behaves as electron transfer mediator, it reacts with HRP-II to release HRP and a enhance (PTZ-343) radical. The enhancer (PTZ-343) radical quickly oxidizes luminol anions to induce light emission[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

PTZ-343 Related Antibodies
Molecular Weight

343.40

Formula

C15H14NNaO3S2
CAS No.
Appearance

Solid

Color

White to off-white

SMILES

O=S(CCCN1C2=C(C=CC=C2)SC3=CC=CC=C13)(O[Na])=O
Shipping

Room temperature in continental US; may vary elsewhere.
Storage

-20°C, sealed storage, away from moisture and light

*In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light)

Solvent & Solubility
In Vitro: 

H2O : 125 mg/mL (364.01 mM; Need ultrasonic)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 2.9121 mL 14.5603 mL 29.1206 mL
5 mM 0.5824 mL 2.9121 mL 5.8241 mL
View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

  • Molarity Calculator

  • Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass
=
Concentration
×
Volume
×
Molecular Weight *

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start)

C1

×
Volume (start)

V1

=
Concentration (final)

C2

×
Volume (final)

V2

In Vivo:

For the following dissolution methods, please prepare the working solution directly. It is recommended to prepare fresh solutions and use them promptly within a short period of time.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

  • Protocol 1

    Add each solvent one by one:  PBS

    Solubility: 50 mg/mL (145.60 mM); Clear solution; Need ultrasonic

In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

g

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Calculation results:
Working solution concentration: mg/mL
This product has good water solubility, please refer to the measured solubility data in water/PBS/Saline for details.
The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only.If necessary, please contact MedChemExpress (MCE).
Purity & Documentation
References

Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
H2O 1 mM 2.9121 mL 14.5603 mL 29.1206 mL 72.8014 mL
5 mM 0.5824 mL 2.9121 mL 5.8241 mL 14.5603 mL
10 mM 0.2912 mL 1.4560 mL 2.9121 mL 7.2801 mL
15 mM 0.1941 mL 0.9707 mL 1.9414 mL 4.8534 mL
20 mM 0.1456 mL 0.7280 mL 1.4560 mL 3.6401 mL
25 mM 0.1165 mL 0.5824 mL 1.1648 mL 2.9121 mL
30 mM 0.0971 mL 0.4853 mL 0.9707 mL 2.4267 mL
40 mM 0.0728 mL 0.3640 mL 0.7280 mL 1.8200 mL
50 mM 0.0582 mL 0.2912 mL 0.5824 mL 1.4560 mL
60 mM 0.0485 mL 0.2427 mL 0.4853 mL 1.2134 mL
80 mM 0.0364 mL 0.1820 mL 0.3640 mL 0.9100 mL
100 mM 0.0291 mL 0.1456 mL 0.2912 mL 0.7280 mL

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

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PTZ-343 Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

PTZ-343101199-38-63-(10′-Phenothiazinyl)propane-1-sulfonateSPTZPTZ343PTZ 343OthersChemiluminescentEnhancerluminollight outputsandwich enzyme-linked immunosorbentWestern blotassayInhibitorinhibitorinhibit

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