A novel label-free electrochemical immunosensor for the detection of heat shock protein 70 of lung adenocarcinoma cell line following paclitaxel treatment using l-cysteine-functionalized Au@MnO2/MoO3 nanocomposites

  • RSC Adv. 2023 Oct 12;13(43):29847-29861. doi: 10.1039/d3ra03620k.
Yi-An Chen  1 ,  Ming-You Shie  1  2  3 ,  Chia-Che Ho  4 ,  Sheng-Wen Ye  2 ,  I-Wen Peter Chen  5 ,  Yu-Yin Shih  1 ,  Yu-Fang Shen  4 ,  Yi-Wen Chen  1  2  4  6
Affiliations
  • 1. x-Dimension Center for Medical Research and Translation, China Medical University Hospital Taichung City 404332 Taiwan.
  • 2. The Master Program for Biomedical Engineering, China Medical University Taichung City 406040 Taiwan.
  • 3. Department of Biomedical Engineering, China Medical University Taichung City 40447 Taiwan.
  • 4. Department of Bioinformatics and Medical Engineering, Asia University Taichung City 41354 Taiwan.
  • 5. Department of Chemistry, National Cheng Kung University Tainan 70101 Taiwan.
  • 6. Graduate Institute of Biomedical Sciences, China Medical University Taichung City 40447 Taiwan.
PMID: 37842680 DOI: 10.1039/d3ra03620k
Abstract

The future trend in achieving precision medicine involves the development of non-invasive Cancer biomarker sensors that offer high accuracy, low cost, and time-saving benefits for risk clarification, early detection, disease detection, and therapeutic monitoring. A facile approach for the synthesis of MoO3 nanosheets was developed by thermally oxidizing MoS2 nanosheets in air followed by thermal annealing. Subsequently, Au@MnO2 nanocomposites were prepared using a combined hydrothermal process and in situ chemical synthesis. In this study, we present a novel immunosensor design strategy involving the immobilization of antiHSP70 antibodies on Au@MnO2/MoO3 nanocomposites modified on a screen-printed electrode (SPE) using EDC/NHS chemistry. This study establishes HSP70 as a potential biomarker for monitoring therapeutic response during Anticancer therapy. Impedance measurements of HSP70 on the Au@MnO2/MoO3/SPE immunosensor using EIS showed an increase in impedance with an increase in HSP70 concentration. The electrochemical immunosensor demonstrated a good linear response in the range of 0.001 to 1000 ng mL-1 with a detection limit of 0.17 pg mL-1 under optimal conditions. Moreover, the immunosensor was effective in detecting HSP70 at low concentrations in a lung adenocarcinoma cell line following Paclitaxel treatment, indicating its potential for early detection of the HSP70 biomarker in organ-on-a-chip and clinical applications.

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