Functional MTTP and apoB for VLDL secretion in chicken ovaries support small follicle development under heat stress
- J Therm Biol. 2025 Oct:133:104298. doi: 10.1016/j.jtherbio.2025.104298.
- 1. Department of Animal Science, National Chung Hsing University, Taichung, 402202, Taiwan; Department of Animal Science and Fisheries, Rajamangala University of Technology Lanna, Lampang, Thailand.
- 2. Center for Molecular Medicine, China Medical University Hospital, Taichung, 406040, Taiwan; Research Center for Cancer Biology, China Medical University, Taichung, 406040, Taiwan; Cancer Biology and Precision Therapeutics Center, China Medical University, Taichung, 406040, Taiwan.
- 3. Department of Animal Science, National Chung Hsing University, Taichung, 402202, Taiwan.
- 4. Department of Animal Science and Biotechnology, Tunghai University, Taichung, 407224, Taiwan.
- 5. Department of Animal Science, National Chung Hsing University, Taichung, 402202, Taiwan; The iEGG and Animal Biotechnology Center and Rong Hsing Research Center for Translational Medicine, National Chung Hsing University, Taichung, 402202, Taiwan; Professional Mater Program of Agricultural Business Management, National Chung Hsing University, Taichung, 402202, Taiwan.
- 6. Department of Poultry Science, Texas A&M University, College Station, TX, 77843, USA.
- 7. Center for Molecular Medicine, China Medical University Hospital, Taichung, 406040, Taiwan; Research Center for Cancer Biology, China Medical University, Taichung, 406040, Taiwan; Cancer Biology and Precision Therapeutics Center, China Medical University, Taichung, 406040, Taiwan. Electronic address: [email protected].
- 8. Department of Animal Science, National Chung Hsing University, Taichung, 402202, Taiwan; The iEGG and Animal Biotechnology Center and Rong Hsing Research Center for Translational Medicine, National Chung Hsing University, Taichung, 402202, Taiwan; i-Center for Advanced Science and Technology (iCAST), National Chung Hsing University, Taichung, 402202, Taiwan. Electronic address: [email protected].
By various assessments, the previous study has unequivocally concluded functional apoB and MTTP (microsomal triglyceride transfer protein) for VLDL production in chicken ovaries. The present study sought to use whole tissue culture to define the role of VLDL secretion by small yellow follicles (SYFs) along their development under normal and heat stress (HS) conditions. Under thermoneutral conditions (39 °C), chicken SYFs increased MTTP activity, apoB expression and VLDL secretion, while underwent cell Apoptosis along the time course. Despite relieved ER stress and protein ubiquitinylation, inhibition of VLDL secretion by Lomitapide and Mipomersen greatly increased triglyceride accumulation, impaired estradiol production and cell proliferation, and accelerated cell Apoptosis in accordance with upregulated Caspase 3/7 activity, JNK activation, protein carbonylation, and MDA accumulation. Exposure to HS at 44 °C boosted cell Apoptosis in a duration-dependent manner. Acute HS for 3 h enhanced VLDL secretion, impaired estradiol production and cell proliferation, and promoted IL-1b production, oxidative damages, and cell Apoptosis, whereas except MDA content and cell proliferation, the detrimental effects were halted after 13 h recovery. Lomitapide and Mipomersen augmented lipid accumulation, oxidative stress, inflammatory response, and exacerbated transient impairment of estradiol secretion and cell proliferation in SYFs under 3 h HS and after recovery, but failed to rescue cell viability despite relieved ER and proteostatic stress. In conclusion, routine secretion of VLDL by SYFs serves as an intrinsic mechanism to sustain cell viability and functions to support the whole program required for follicle development, while under HS, this mechanism provisionally rescues steroidogenesis and cell proliferation.
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Cat. No.Product NameDescriptionTargetResearch Area
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Research Areas: Infection