Q-Peptide 

Q-Peptide is an angiopoietin-1 derived peptide (QHREDGS). Q-Peptide interacts with β1-integrin, binds to integrins on the surface of osteoblasts, and serves as an acyl donor substrate for Streptomyces mobaraensis transglutaminase. Q-Peptide activates Akt, MAPKp42/44, ILK, ERK1/2, and downregulates caspase-3/7. Q-Peptide inhibits cell apoptosis, enhances cell adhesion and migration, and promotes osteoblast differentiation, bone matrix deposition and mineralization. Q-Peptide can be used in studies related to myocardial infarction, bone regeneration, diabetic wound repair and human induced pluripotent stem cells^{[1][2][3][4][5]}.

Q-Peptide (QHREDGS) (5-500 μM; 5 continuous passages, 7 days) improves human induced pluripotent stem cell (BJ1D, 0901B, IMR90) expansion and viability in feeder-dependent culture by reducing caspase-dependent apoptosis, without altering proliferation, while having no significant effect on human embryonic stem cells (H9)^[1].
Q-Peptide (50 μM; 5 continuous passages) preserves the in vitro pluripotency of human induced pluripotent stem cells (BJ1D, 0901B)^[1].
Q-Peptide (5-500 μM; 48h) significantly increases colony number and size in feeder-free human induced pluripotent stem cells cultured on Matrigel^[1].
Q-Peptide (50 μM; 0min-24h) increases integrin-linked kinase (ILK) expression, and increases expression and activation of extracellular signal-regulated kinases 1/2 (ERK1/2) across multiple time points, without affecting AKT activation^[1].
Q-Peptide (7.2-29.0 mM; 6h) dose-dependently enhances human induced pluripotent stem cell adhesion to non-adhesive PEG hydrogels, and this interaction is mediated by β1-integrins^[1].
Q-Peptide (200 μM; 40 min) potently acts as a substrate for Streptomyces mobaraensis transglutaminase (STG), with a cross-linking conversion yield of 93.0%^[4].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Q-Peptide (2.2 nmol; topical; single application) significantly accelerates diabetic wound healing in db/db mice by promoting re-epithelialization and granulation tissue formation; it is significantly superior to clinically approved collagen dressings in accelerating diabetic wound healing in db/db mice, achieving near-complete wound closure by day 21^[3].
QP-derived functional peptide hydrogel (1% w/v, for local single injection) mediated mesenchymal stem cell transplantation can improve cardiac function (EF and FS), reduce infarct size, collagen content and cardiomyocyte apoptosis in rat myocardial infarction models. This treatment also elevates the survival rate of transplanted cells and promotes angiogenesis. Ctf1 pretreatment can further enhance the therapeutic effects of QP-based hydrogel mediated mesenchymal stem cell transplantation^[5].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

827.80

C₃₁H₄₉N₁₃O₁₄

1361235-89-3

Solid

White to off-white

Gln-His-Arg-Glu-Asp-Gly-Ser

QHREDGS

Room temperature in continental US; may vary elsewhere.

Sealed storage, away from moisture

*In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

• [1]. Dang LT, et al. Inhibition of apoptosis in human induced pluripotent stem cells during expansion in a defined culture using angiopoietin-1 derived peptide QHREDGS. Biomaterials. 2014;35(27):7786-7799.

  [2]. Feric N, et al. Angiopoietin-1 peptide QHREDGS promotes osteoblast differentiation, bone matrix deposition and mineralization on biomedical materials. Biomater Sci. 2014;2(10):1384-1398.

  [3]. Xiao Y, et al. Diabetic wound regeneration using peptide-modified hydrogels to target re-epithelialization. Proc Natl Acad Sci U S A. 2016;113(40):E5792-E5801.

  [4]. Lee JH, et al. Glutamine (Q)-peptide screening for transglutaminase reaction using mRNA display. Biotechnol Bioeng. 2013;110(2):353-362.

  [5]. Cai H, et al. Self-assembling peptide modified with QHREDGS as a novel delivery system for mesenchymal stem cell transplantation after myocardial infarction. FASEB J. 2019;33(7):8306-8320.  [Content Brief]