R 8231

R 8231 (at a maximum concentration of 0.5 mM) does not significantly inhibit alkaline phosphatase activity in fixed HeLa cells, indicating that alkaline phosphatase derived from HeLa cells is insensitive to R 8231^[1].
After treatment of rat tissue sections with R 8231 (0.1 mM; 37°C; pre-incubation for 1 h) and subsequent transfer to an inhibitor-free reaction solution, the alkaline phosphatase reaction product does not decrease, indicating that the inhibitory effect of R 8231 is reversible^[1].
R 8231 (1 μM) potently inhibits purified alkaline phosphatase isozymes derived from human liver, bone, kidney and spleen via an uncompetitive mechanism. Under the tested conditions, the maximum inhibition rate of R 8231 against human liver alkaline phosphatase reaches 68%, while it exerts weak effects on alkaline phosphatase isozymes derived from human intestine and placenta^[2].
R 8231 (10-100 μM; 6 days) almost completely inhibits the activity of bone alkaline phosphatase in rat embryos, but does not inhibit the growth of rat embryonic femur explants under in vitro culture conditions^[3].
R 8231 (0.5 mM) inhibits the interference of non-specific alkaline phosphatase in homogenates of secretory-stage enamel organs from Sprague-Dawley rats, and thus is used for the specific detection of Ca²⁺-stimulated ATPase activity with an optimal pH of 8.2. In standard enzyme activity assays, the reaction duration ranges from 2-15 min; in ATP metabolism time-course assays with a maximum duration of 30 min, the reaction in the system containing R 8231 proceeds slightly slower, and the production of AMP and adenosine is reduced^[4].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only. Further protocols information, click here.