(R)-Lirinidine
(R)-Lirinidine ((-)-Lirinidine) is an aporphine-type alkaloid found in the flower buds and leaves of Nelumbo nucifera. (R)-Lirinidineand inhibits melanogenesis with an IC50 of 19.3 μM. (R)-Lirinidine exerts cytotoxic effects at 100 μM.
For research use only. We do not sell to patients.
- CAS No.: 37082-15-8
- Formula: C18H19NO2
- Molecular Weight:281.35
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Storage:
Please store the product under the recommended conditions in the Certificate of Analysis.
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Biological Activity
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Chemical Information
- Protocols
- Purity & Documentation
- References
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Help & FAQs
Biological Activity
Description
Cellular Effect
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Cell Line
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Type | Value | Description | References |
|---|---|---|---|---|
| B16-4A5 | IC50 |
19.3 μM
Compound: 7, 5-demethylnuciferine
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Inhibition of melanogenesis in mouse B16-4A5 cells after 72 hrs by spectrophotometry
Inhibition of melanogenesis in mouse B16-4A5 cells after 72 hrs by spectrophotometry
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[PMID: 23270663] |
In Vitro
(R)-Lirinidine (Compound 7) (3-100 μM; 72 h) potently inhibits melanogenesis in theophylline-stimulated murine B16 melanoma 4A5 cells with an IC50 of 19.3 μM, and exhibits cytotoxicity at 100 μM[1].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only. Further protocols information, click here.
Chemical Information
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CAS No. 37082-15-8
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Molecular Weight 281.35
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Formula C18H19NO2
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SMILES
OC1=C(OC)C=C2CCN(C)[C@]3([H])CC4=CC=CC=C4C1=C23
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Synonyms
(-)-Lirinidine
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Structure Classification
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Initial Source
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
Please store the product under the recommended conditions in the Certificate of Analysis.
Protocols
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Cell Cytotoxicity Assay
Cytotoxicity assays are usually based on the assessment of cell membrane damage, which can also be indirectly detected by measuring cell viability. Detection methods include MTT assay, CKK-8 assay, LDH assay and ATP assay, etc.
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Cell Viability Determination by MTT Colorimetric Assay
The following protocol uses the MTT colorimetric assay as a classic literature-established method for assessing cell viability/metabolic activity in cultured mammalian cells. MTT[3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide] is reduced by metabolically active cells to a colored formazan product; the amount of formazan is quantified spectrophotometrically and provides an indirect measure of metabolically active viable cells. Importantly, MTT reduction reflects cellular oxidoreductase/metabolic activity rather than an absolute direct count of living cells, so changes in cellular metabolism can alter the signal independently of cell number.
Purity & Documentation
References
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
(R)-Lirinidine Related Classifications
HY-N2307B Related Classifications
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