2. Metabolic Enzyme/Protease Apoptosis NF-κB Immunology/Inflammation
  3. Proteasome Apoptosis Reactive Oxygen Species
  4. RA375

RA375 is a RPN13 (26S proteasome regulatory subunit) inhibitor. RA375 activates UPR signaling, ROS production and apoptosis. RA375 exhibits ten-fold greater activity against cancer lines than RA190, reflecting its nitro ring substituents and the addition of a chloroacetamide warhead.

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RA375 Chemical Structure

RA375 Chemical Structure

CAS No. : 2649154-57-2

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1 mg USD 75 In-stock
5 mg USD 185 In-stock
10 mg USD 300 In-stock
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Based on 1 publication(s) in Google Scholar

RA375 Related Antibodies

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  • Purity & Documentation

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Description

RA375 is a RPN13 (26S proteasome regulatory subunit) inhibitor. RA375 activates UPR signaling, ROS production and apoptosis. RA375 exhibits ten-fold greater activity against cancer lines than RA190, reflecting its nitro ring substituents and the addition of a chloroacetamide warhead[1].

In Vivo

RA375 (10 mg/kg, ip) inhibits proteasome function and reduces ovarian tumor burden in mice[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model: RA375 (10 mg/kg, ip) inhibits proteasome function and reduces ovarian tumor burden in mice[1].
Dosage: 10 mg/kg.
Administration: IP for a 5 days on, 2 days off cycle for two weeks.
Result: Reduced ovarian tumor burden in mice.
Animal Model: Female Balb/c mice[1].
Dosage: 5, 10, 20, 40, 60, 100 mg/kg (Pharmacological Analysis).
Administration: IP single dose.
Result: The dose of 40 mg/kg on alternate days for two weeks produced no observable toxicities or weight loss.
Molecular Weight

589.00

Formula

C30H25ClN4O7
CAS No.
Appearance

Solid

Color

Light yellow to yellow

SMILES

O=C([C@@H](NC(CCl)=O)CC1=CC=CC=C1)N(C/C(C/2=O)=C\C3=CC=C([N+]([O-])=O)C=C3)CC2=C/C4=CC=C([N+]([O-])=O)C=C4
Shipping

Room temperature in continental US; may vary elsewhere.
Storage
Powder -20°C 3 years
In solvent -80°C 6 months
-20°C 1 month
Solvent & Solubility
In Vitro: 

DMSO : 100 mg/mL (169.78 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 1.6978 mL 8.4890 mL 16.9779 mL
5 mM 0.3396 mL 1.6978 mL 3.3956 mL
View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

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Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

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Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
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Working solution concentration: mg/mL
Purity & Documentation
References

Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
DMSO 1 mM 1.6978 mL 8.4890 mL 16.9779 mL 42.4448 mL
5 mM 0.3396 mL 1.6978 mL 3.3956 mL 8.4890 mL
10 mM 0.1698 mL 0.8489 mL 1.6978 mL 4.2445 mL
15 mM 0.1132 mL 0.5659 mL 1.1319 mL 2.8297 mL
20 mM 0.0849 mL 0.4244 mL 0.8489 mL 2.1222 mL
25 mM 0.0679 mL 0.3396 mL 0.6791 mL 1.6978 mL
30 mM 0.0566 mL 0.2830 mL 0.5659 mL 1.4148 mL
40 mM 0.0424 mL 0.2122 mL 0.4244 mL 1.0611 mL
50 mM 0.0340 mL 0.1698 mL 0.3396 mL 0.8489 mL
60 mM 0.0283 mL 0.1415 mL 0.2830 mL 0.7074 mL
80 mM 0.0212 mL 0.1061 mL 0.2122 mL 0.5306 mL
100 mM 0.0170 mL 0.0849 mL 0.1698 mL 0.4244 mL
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RA375 Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

RA3752649154-57-2RA 375RA-375ProteasomeApoptosisReactive Oxygen Species26SproteasomeovariantumorInhibitorinhibitorinhibit

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