Rebaudioside B
Based on 1 Customer Validation
Rebaudioside B is the minor constituent isolated from the leaves of Stevia rebaudiana Bertoni. Rebaudioside B tastes about 150 times sweeter than sucrose. Rebaudioside B can hydrolysis to steviol.
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- Reinheit: 98.51%
- CAS. Nr.: 58543-17-2
- Formel: C38H60O18
- Molecular Weight:804.87
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Speicherung:
4°C, protect from light
* In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)
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Biologische Aktivität
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Chemical Information
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Biologische Aktivität
Incubation of fecal homogenates with Rebaudioside B at 0.2 mg/mL results in a slightly lower level of metabolism to steviol relative to Rebaudioside A and D. After 8 h incubation in fecal homogenates, Rebaudioside B is metabolized to steviol from 71.5% to 87.3% in males and from 58.1% to 89.7% in females. Metabolism of Rebaudioside B to steviol is essentially complete after 24 h incubation[2].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
Chemical Information
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CAS. Nr. 58543-17-2
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Appearance Solid
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Molecular Weight 804.87
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Formel C38H60O18
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Color White to off-white
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SMILES
C[C@]12[C@@]3([H])[C@@](C4)(CC[C@]1([H])[C@@](C)(CCC2)C(O)=O)C[C@@](C4=C)(O[C@@]5([H])[C@@H]([C@H]([C@H](O)[C@@H](CO)O5)O[C@]6([H])O[C@@H]([C@@H](O)[C@H](O)[C@H]6O)CO)O[C@]7([H])O[C@@H]([C@@H](O)[C@H](O)[C@H]7O)CO)CC3
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Structure Classification
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Initial Source
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Versand
Room temperature in continental US; may vary elsewhere.
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Speicherung
4°C, protect from light
* In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)
Lösungsmittel & Löslichkeit
DMSO : 100 mg/mL (124.24 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Konzentration (Stammlösung) × Volumen (Stammlösung) = Konzentration (Ziellösung) × Volumen (Ziellösung)
Select the appropriate dissolution method based on your experimental animal and administration route.
- For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
- To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
- The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.
Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 2.5 mg/mL (3.11 mM); Clear solution
This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: ≥ 2.5 mg/mL (3.11 mM); Clear solution
This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
Please enter the basic information of animal experiments:
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Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
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%DMSO +
Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
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%+
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+%Tween-80 + +
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%Saline +
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Working solution concentration: 0.22 mg/mL
Method for preparing stock solution: mg drug dissolved in μL DMSO. Stock solution concentration: mg/mL. * In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)
1. Take μL DMSO stock solution;
2. Add μL .
μL , mix evenly;
3. Then add μL Tween 80, mix evenly;
4. Then add μL
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
Reinheit & Dokumentation
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Data Sheet (279 KB)
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SDS (393 KB)
- English - EN (393 KB)
- Français - FR (393 KB)
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Handling Instructions (2659 KB)
Verweise
[1]. Prakash I, et al. Catalytic hydrogenation of the sweet principles of Stevia rebaudiana, Rebaudioside B, Rebaudioside C, and Rebaudioside D and sensory evaluation of their reduced derivatives. Int J Mol Sci. 2012 Nov 16;13(11):15126-36. [Content Brief]
[2]. Purkayastha S, et al. In vitro metabolism of rebaudioside B, D, and M under anaerobic conditions: comparison with rebaudioside A. Regul Toxicol Pharmacol. 2014 Mar;68(2):259-68. [Content Brief]
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| DMSO | 1 mM | 1.2424 mL | 6.2122 mL | 12.4244 mL | 31.0609 mL |
| 5 mM | 0.2485 mL | 1.2424 mL | 2.4849 mL | 6.2122 mL | |
| 10 mM | 0.1242 mL | 0.6212 mL | 1.2424 mL | 3.1061 mL | |
| 15 mM | 0.0828 mL | 0.4141 mL | 0.8283 mL | 2.0707 mL | |
| 20 mM | 0.0621 mL | 0.3106 mL | 0.6212 mL | 1.5530 mL | |
| 25 mM | 0.0497 mL | 0.2485 mL | 0.4970 mL | 1.2424 mL | |
| 30 mM | 0.0414 mL | 0.2071 mL | 0.4141 mL | 1.0354 mL | |
| 40 mM | 0.0311 mL | 0.1553 mL | 0.3106 mL | 0.7765 mL | |
| 50 mM | 0.0248 mL | 0.1242 mL | 0.2485 mL | 0.6212 mL | |
| 60 mM | 0.0207 mL | 0.1035 mL | 0.2071 mL | 0.5177 mL | |
| 80 mM | 0.0155 mL | 0.0777 mL | 0.1553 mL | 0.3883 mL | |
| 100 mM | 0.0124 mL | 0.0621 mL | 0.1242 mL | 0.3106 mL |
Rebaudioside B Related Classifications
HY-N6808 Related Classifications
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