Riparin
Based on 1 Customer Validation
Riparin is a nonspecific smooth muscle relaxant. Riparin exerts spasmolytic effect by inhibiting Ca2+ influx and inhibiting the release of intracellular calcium ion stores.
For research use only. We do not sell to patients.
- Purity: 98.00%
- CAS No.: 112356-54-4
- Formula: C16H17NO4
- Molecular Weight:287.31
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Storage:
4°C, protect from light
* In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
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Biological Activity
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Chemical Information
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Solvent & Solubility
- Purity & Documentation
- References
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Help & FAQs
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Membrane Transporter/Ion Channel Compound Library
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Human Metabolite Library
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All Calcium Channel Isoforms
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Biological Activity
In rat depolarized uterus, Riparin inhibits in a reversible and noncompetitive manner CaCl2-induced contraction, a response mediated through voltage-dependent Ca2+ channels. The pD2 value for Riparin is 4.98. Riparin is ineffective in suppressing noradrenaline-induced sustained contractions of rabbit aortic strips[1].
In the aorta, Riparin (IC50 of 10.1 μM) inhibits intracellular calcium-dependent transient contractions[1].
In guinea-pig alveolar leucocytes, Riparin (IC50 3.2 μM) inhibits intracellular Ca2+ accumulation induced by the calcium ionophore A23187[1].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
Chemical Information
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CAS No. 112356-54-4
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Appearance Solid
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Molecular Weight 287.31
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Formula C16H17NO4
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Color White to off-white
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SMILES
O=C(C1=C(C=CC=C1O)O)NCCC2=CC=C(C=C2)OC
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Synonyms
Riparin III
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
4°C, protect from light
* In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)
Solvent & Solubility
DMSO : 100 mg/mL (348.06 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Select the appropriate dissolution method based on your experimental animal and administration route.
- For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
- To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
- The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.
Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 2.5 mg/mL (8.70 mM); Clear solution
This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: ≥ 2.5 mg/mL (8.70 mM); Clear solution
This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
Please enter the basic information of animal experiments:
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Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
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%DMSO +
Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
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%+
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+%Tween-80 + +
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%Saline +
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Working solution concentration: 0.22 mg/mL
Method for preparing stock solution: mg drug dissolved in μL DMSO. Stock solution concentration: mg/mL. * In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)
1. Take μL DMSO stock solution;
2. Add μL .
μL , mix evenly;
3. Then add μL Tween 80, mix evenly;
4. Then add μL
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
Purity & Documentation
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Data Sheet (272 KB)
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SDS (393 KB)
- English - EN (393 KB)
- Français - FR (393 KB)
- Deutsch - DE (393 KB)
- Norwegian - NO (393 KB)
- Español - ES (393 KB)
- Swedish - SV (393 KB)
- Italian - IT (393 KB)
- Korean - KR (393 KB)
- Portuguese - PT (393 KB)
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Handling Instructions (2659 KB)
References
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| DMSO | 1 mM | 3.4806 mL | 17.4028 mL | 34.8056 mL | 87.0140 mL |
| 5 mM | 0.6961 mL | 3.4806 mL | 6.9611 mL | 17.4028 mL | |
| 10 mM | 0.3481 mL | 1.7403 mL | 3.4806 mL | 8.7014 mL | |
| 15 mM | 0.2320 mL | 1.1602 mL | 2.3204 mL | 5.8009 mL | |
| 20 mM | 0.1740 mL | 0.8701 mL | 1.7403 mL | 4.3507 mL | |
| 25 mM | 0.1392 mL | 0.6961 mL | 1.3922 mL | 3.4806 mL | |
| 30 mM | 0.1160 mL | 0.5801 mL | 1.1602 mL | 2.9005 mL | |
| 40 mM | 0.0870 mL | 0.4351 mL | 0.8701 mL | 2.1754 mL | |
| 50 mM | 0.0696 mL | 0.3481 mL | 0.6961 mL | 1.7403 mL | |
| 60 mM | 0.0580 mL | 0.2900 mL | 0.5801 mL | 1.4502 mL | |
| 80 mM | 0.0435 mL | 0.2175 mL | 0.4351 mL | 1.0877 mL | |
| 100 mM | 0.0348 mL | 0.1740 mL | 0.3481 mL | 0.8701 mL |
Riparin Related Classifications
HY-121966 Related Classifications
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