RP-832c 

RP-832c is a synthetic analogue of host defense peptides (HDP), targeting the mannose receptor CD206 on the surface of M2 polarized macrophages (K_d = 3.5 μM). RP-832c binding to CD206 induces a significant conformational change in the receptor, activating signaling pathways that lead to rapid apoptosis and repolarization of CD206-positive M2 macrophages to an M1 phenotype. RP-832c treatment significantly reduces CD206 gene expression in M2 macrophages while transiently increasing expression of TNF-α, a marker for M1 macrophages. RP-832c is used for the studies of T-cell lymphoma (CTCL) and idiopathic pulmonary fibrosis (IPF)^[1][2].

RP-832c (0-100 μM) shows dose-dependent inhibition on M2 polarized bone marrow-derived macrophages (BMDM), with an IC₅₀ value of 6.184 μM, producing minimal cytotoxicity on M1 polarized macrophages and has no cytotoxicity towards two different human fetal lung fibroblast cell lines, MRC5 and IMR9^[2].
RP-832c (10 μM, 24 h) significantly reduces the expression of CD206 in M2 macrophages, and briefly increases the expression of TNF-α^[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

RP-832c (i.p., once daily for 10 days) in the CTCL tumor microenvironment inhibits tumor growth by inducing a phenotypic shift from immunosuppressive M2-like to anti-tumor M1-like macrophages^[1].
RP-832c (5-10 mg/kg, s.c., once daily for 21 days) demonstrates significant preventive and therapeutic effects in the mouse model of pulmonary fibrosis^[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

1367.60

C₆₈H₉₄N₂₀O₁₁

2375823-45-1

Solid

White to off-white

Arg-Trp-Lys-Phe-Gly-Gly-Phe-Lys-Trp-Arg

RWKFGGFKWR

Room temperature in continental US; may vary elsewhere.

Sealed storage, away from moisture

*In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

• [1]. Carla Portocarrero, et al; Targeting Immunosuppressive Macrophages in the Cutaneous T-Cell Lymphoma Microenvironment. Blood 2022; 140 (Supplement 1): 3146–3147.

  [2]. Ghebremedhin A, et al. A Novel CD206 Targeting Peptide Inhibits Bleomycin-Induced Pulmonary Fibrosis in Mice. Cells. 2023 Apr 26;12(9):1254.  [Content Brief]