RP-832c
Based on 1 Customer Validation
RP-832c is a synthetic analogue of host defense peptides (HDP), targeting the mannose receptor CD206 on the surface of M2 polarized macrophages (Kd = 3.5 μM). RP-832c binding to CD206 induces a significant conformational change in the receptor, activating signaling pathways that lead to rapid apoptosis and repolarization of CD206-positive M2 macrophages to an M1 phenotype. RP-832c treatment significantly reduces CD206 gene expression in M2 macrophages while transiently increasing expression of TNF-α, a marker for M1 macrophages. RP-832c is used for the studies of T-cell lymphoma (CTCL) and idiopathic pulmonary fibrosis (IPF).
For research use only. We do not sell to patients.
- Purity: 99.86%
- CAS No.: 2375823-45-1
- Formula: C68H94N20O11
- Molecular Weight:1367.60
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Storage:
Sealed storage, away from moisture.
Powder -80°C, 2 years , -20°C, 1 year* In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)
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Biological Activity
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Chemical Information
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Solvent & Solubility
- Purity & Documentation
- References
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Help & FAQs
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Apoptosis Compound Library
HY-L003
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Immunology/Inflammation Compound Library
HY-L007
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Anti-Cancer Compound Library
HY-L025
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Anti-Blood Cancer Compound Library
HY-L079
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Angiogenesis-Related Compound Library
HY-L088
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Peptide Library
HY-L105
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Anti-Pulmonary Fibrosis Compound Library
HY-L125
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Cancer Stem Cells Compound Library
HY-L135
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Pain-Related Compound Library
HY-L139
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Membrane Protein-targeted Compound Library
HY-L149
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Bioactive Compound Library
HY-L001
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Non-Alcoholic Fatty Liver Disease (NAFLD) Compound Library
HY-L199
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High-Throughput Bioactive Compound Library
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Biological Activity
RP-832c (0-100 μM) shows dose-dependent inhibition on M2 polarized bone marrow-derived macrophages (BMDM), with an IC50 value of 6.184 μM, producing minimal cytotoxicity on M1 polarized macrophages and has no cytotoxicity towards two different human fetal lung fibroblast cell lines, MRC5 and IMR9[2].
RP-832c (10 μM, 24 h) significantly reduces the expression of CD206 in M2 macrophages, and briefly increases the expression of TNF-α[2].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
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Cell Line:M2 macrophages
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Concentration:10 μM
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Incubation Time:0, 2, 8 and 24 h
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Result:Decreased CD206 gene expression.
Resulted in an initial increase in TNF-α gene expression.
RP-832c (5-10 mg/kg, s.c., once daily for 21 days) demonstrates significant preventive and therapeutic effects in the mouse model of pulmonary fibrosis[2].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
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Animal Model:Bleomycin (HY-108345) induced lung fibrosis model models established in 6-8-week-old C57BL/6 mice[2]
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Dosage:5 and 10 mg/kg for prevention group and 10 mg/kg for treatment group
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Administration:Subcutaneous injection (s.c.), once daily for 21 days
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Result:significantly reduced collagen deposition in a dose-dependent manner with the alveolar structure well-preserved and the degree of fibrosis mild in prevention group.
Significantly reduces the degree of already formed fibrosis , reduced TNF-α, IL-6, IL-10, IFN-γ, CXCL1/2, etc and the expression of TGF-β1 and MMP-13 in treatment group.
Observed no significant toxicity at a dose of up to 50 mg/kg.
Chemical Information
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CAS No. 2375823-45-1
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Appearance Solid
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Molecular Weight 1367.60
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Formula C68H94N20O11
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Color White to off-white
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Sequence
Arg-Trp-Lys-Phe-Gly-Gly-Phe-Lys-Trp-Arg
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Sequence Shortening
RWKFGGFKWR
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
Sealed storage, away from moisture
Powder -80°C 2 years -20°C 1 year * In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)
Solvent & Solubility
DMSO : 25 mg/mL (18.28 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Purity & Documentation
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Data Sheet (275 KB)
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SDS (252 KB)
- English - EN (252 KB)
- Français - FR (252 KB)
- Deutsch - DE (252 KB)
- Norwegian - NO (252 KB)
- Español - ES (252 KB)
- Swedish - SV (252 KB)
- Italian - IT (252 KB)
- Korean - KR (252 KB)
- Portuguese - PT (252 KB)
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Handling Instructions (2659 KB)
References
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| DMSO | 1 mM | 0.7312 mL | 3.6560 mL | 7.3121 mL | 18.2802 mL |
| 5 mM | 0.1462 mL | 0.7312 mL | 1.4624 mL | 3.6560 mL | |
| 10 mM | 0.0731 mL | 0.3656 mL | 0.7312 mL | 1.8280 mL | |
| 15 mM | 0.0487 mL | 0.2437 mL | 0.4875 mL | 1.2187 mL |
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