(S)-6-Methylnicotine
Based on 1 Customer Validation
(S)-6-Methylnicotine is a nicotinic acetylcholine receptor (nAChR) agonist that exhibits agonistic activity on isolated guinea pig ileum. (S)-6-Methylnicotine produces sensations identical to those of nicotine and has addictive potential. (S)-6-Methylnicotine shows high cytotoxicity against human bronchial epithelial cells and exerts a weak effect on the upregulation of lung cancer-related proteins. (S)-6-Methylnicotine can be used in the research of respiratory system diseases.
For research use only. We do not sell to patients.
- Purity : 98.0%
- CAS No.: 13270-56-9
- Formula: C11H16N2
- Molecular Weight:176.26
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Storage:
4°C, protect from light
* In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
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Biological Activity
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Chemical Information
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Solvent & Solubility
- Purity & Documentation
- References
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Help & FAQs
Biological Activity
Description
In Vitro
(S)-6-Methylnicotine is a naturally occurring minor tobacco alkaloid present in additive-free cured leaf tobaccos, smokeless tobaccos, and domestic/foreign/Nigerian cigarettes at an all-sample average level of 0.32 µg per g of tobacco material[1].
(S)-6-Methylnicotine confirms presence in Sweet Spearmint, Blue Razz Ice, and Mango Grapefruit Spree Bar e-liquids, with mass accuracy within 7 ppm of the theoretical [M+H]+ ion value[2].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
Chemical Information
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CAS No. 13270-56-9
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Appearance Liquid (Density: 1.016±0.06 g/cm3)
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Molecular Weight 176.26
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Formula C11H16N2
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Color Colorless to light yellow
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SMILES
CN1[C@H](C2=CN=C(C)C=C2)CCC1
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
4°C, protect from light
* In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)
Solvent & Solubility
In Vitro:
DMSO : 100 mg/mL (567.34 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
In Vivo:
Select the appropriate dissolution method based on your experimental animal and administration route.
- For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
- To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
- The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.
Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 2.5 mg/mL (14.18 mM); Clear solution
This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: ≥ 2.5 mg/mL (14.18 mM); Clear solution
This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:
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Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
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%DMSO +
Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
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%+
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+%Tween-80 + +
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%Saline +
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Working solution concentration: 0.22 mg/mL
Method for preparing stock solution: mg drug dissolved in μL DMSO. Stock solution concentration: mg/mL. * In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)
1. Take μL DMSO stock solution;
2. Add μL .
μL , mix evenly;
3. Then add μL Tween 80, mix evenly;
4. Then add μL
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
Purity & Documentation
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Data Sheet (275 KB)
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SDS (394 KB)
- English - EN (394 KB)
- Français - FR (394 KB)
- Deutsch - DE (394 KB)
- Norwegian - NO (394 KB)
- Español - ES (394 KB)
- Swedish - SV (394 KB)
- Italian - IT (394 KB)
- Korean - KR (394 KB)
- Portuguese - PT (394 KB)
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Handling Instructions (2659 KB)
References
[1]. Pankow JF, et al. Levels of the nicotine analog 6-methyl nicotine as a naturally formed tobacco alkaloid in tobacco and tobacco products. Scientific reports. 2025 May 23;15(1):17945. [Content Brief]
[2]. Jordt SE, et al. An electronic cigarette pod system delivering 6-methyl nicotine, a synthetic nicotine analog, marketed in the United States as "PMTA exempt". medRxiv [Preprint]. 2023 Nov 22:2023.11.21.23298778. [Content Brief]
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| DMSO | 1 mM | 5.6734 mL | 28.3672 mL | 56.7344 mL | 141.8359 mL |
| 5 mM | 1.1347 mL | 5.6734 mL | 11.3469 mL | 28.3672 mL | |
| 10 mM | 0.5673 mL | 2.8367 mL | 5.6734 mL | 14.1836 mL | |
| 15 mM | 0.3782 mL | 1.8911 mL | 3.7823 mL | 9.4557 mL | |
| 20 mM | 0.2837 mL | 1.4184 mL | 2.8367 mL | 7.0918 mL | |
| 25 mM | 0.2269 mL | 1.1347 mL | 2.2694 mL | 5.6734 mL | |
| 30 mM | 0.1891 mL | 0.9456 mL | 1.8911 mL | 4.7279 mL | |
| 40 mM | 0.1418 mL | 0.7092 mL | 1.4184 mL | 3.5459 mL | |
| 50 mM | 0.1135 mL | 0.5673 mL | 1.1347 mL | 2.8367 mL | |
| 60 mM | 0.0946 mL | 0.4728 mL | 0.9456 mL | 2.3639 mL | |
| 80 mM | 0.0709 mL | 0.3546 mL | 0.7092 mL | 1.7729 mL | |
| 100 mM | 0.0567 mL | 0.2837 mL | 0.5673 mL | 1.4184 mL |
(S)-6-Methylnicotine Related Classifications
HY-203084 Related Classifications
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