Sanggenon N
Sanggenon N is an isoprenylated flavonoid that can be isolated from the root bark of Morus alba.Sanggenon N has hepatoprotective activities on t-BHP-induced cell cytotoxicity in HepG2 cells with an EC50 of 23.45 μM.
For research use only. We do not sell to patients.
- CAS No.: 92280-12-1
- Formula: C25H26O6
- Molecular Weight:422.47
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Storage:
Please store the product under the recommended conditions in the Certificate of Analysis.
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Biological Activity
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Chemical Information
- Protocols
- Purity & Documentation
- References
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Help & FAQs
Biological Activity
Description
In Vitro
Sanggenon N (Compound 8) ( 0-40 μM; 2 hours) has hepatoprotective effects on t-BHP-induced cytotoxicity in HepG2 cells[1].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only. Further protocols information, click here.
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Cell Line:t-BHP-induced cytotoxicity in HepG2 cells
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Concentration:10,20,40 μM
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Incubation Time:2 hours
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Result:Exhibited protective effects in a dose-dependent manner with an EC50 of 23.45 μM.
Chemical Information
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CAS No. 92280-12-1
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Molecular Weight 422.47
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Formula C25H26O6
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SMILES
O=C1CC(C2=CC=C3C(C=CC(CC/C=C(C)/C)(C)O3)=C2O)OC4=CC(O)=CC(O)=C14
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Structure Classification
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Initial Source
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
Please store the product under the recommended conditions in the Certificate of Analysis.
Protocols
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Cell Cytotoxicity Assay
Cytotoxicity assays are usually based on the assessment of cell membrane damage, which can also be indirectly detected by measuring cell viability. Detection methods include MTT assay, CKK-8 assay, LDH assay and ATP assay, etc.
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Mammalian live/dead viability and cytotoxicity staining
Live/dead viability and cytotoxicity staining assays are based on the simultaneous detection of intracellular esterase activity in metabolically active (viable) cells and membrane integrity loss in non-viable cells. In commonly used dual-staining approaches, membrane-permeant fluorogenic substrates are converted by intracellular esterases into fluorescent products in live cells, while impermeant DNA-binding dyes selectively enter cells with compromised plasma membranes and label nucleic acids in dead or dying cells, enabling discrimination between viable and non-viable populations by fluorescence microscopy or flow cytometry.
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Cell Viability Determination by MTT Colorimetric Assay
The following protocol uses the MTT colorimetric assay as a classic literature-established method for assessing cell viability/metabolic activity in cultured mammalian cells. MTT[3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide] is reduced by metabolically active cells to a colored formazan product; the amount of formazan is quantified spectrophotometrically and provides an indirect measure of metabolically active viable cells. Importantly, MTT reduction reflects cellular oxidoreductase/metabolic activity rather than an absolute direct count of living cells, so changes in cellular metabolism can alter the signal independently of cell number.
Purity & Documentation
References
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Sanggenon N Related Classifications
HY-N1324 Related Classifications
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