INF4E
Based on 1 Customer Validation
INF4E is a potent NLRP3 inflammasome inhibitor. INF4E inhibits caspase-1 and NLRP3 ATPase activities. INF4E shows protection against the IR-induced myocardial injury and dysfunction.
For research use only. We do not sell to patients.
- Purity: 99.38%
- CAS No.: 88039-46-7
- Formula: C12H13ClO3
- Molecular Weight:240.68
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Storage:Pure form -20°C, 3 years , 4°C, 2 years ; In solvent -80°C, 6 months , -20°C, 1 month
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
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Biological Activity
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Chemical Information
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Solvent & Solubility
- Purity & Documentation
- References
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Help & FAQs
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Immunology/Inflammation Compound Library
HY-L007
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NF-κB Signaling Compound Library
HY-L014
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Antioxidant Compound Library
HY-L037
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Oxygen Sensing Compound Library
HY-L045
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Pyroptosis Compound Library
HY-L059
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Antidepressant Compound Library
HY-L108
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Membrane Receptor-targeted Compound Library
HY-L150
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Autoimmune Disease Compound Library
HY-L156
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Inflammasomes related Compound Library
HY-L175
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Radioprotector Library
HY-L178
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Bioactive Compound Library Max
HY-L181
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MCE Bioactive Compound Library
HY-L001V
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Bioactive Compound Library
HY-L001
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High-Throughput Bioactive Compound Library
HY-L205
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Pattern Recognition Receptors Library
HY-L237
Biological Activity
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NLRP3 |
INF4E (compound 9) preventes both ATP- and nigericin-triggered pyroptosis of human THP-1 cells in a time- and concentration-dependent manner[3].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
INF4E attenuates the formation of NLRP3 inflammasome complex was induced by myocardial IR (ischemia/reperfusion) in a time-dependent way[1].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
Chemical Information
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CAS No. 88039-46-7
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Appearance Liquid (Density: 1.222±0.06 g/cm3)
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Molecular Weight 240.68
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Formula C12H13ClO3
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Color Colorless to light yellow
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SMILES
CCOC(C(C(O)C1=C(Cl)C=CC=C1)=C)=O
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
Pure form -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month
Solvent & Solubility
DMSO : ≥ 100 mg/mL (415.49 mM; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
* "≥" means soluble, but saturation unknown.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Select the appropriate dissolution method based on your experimental animal and administration route.
- For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
- To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
- The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.
Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 2.5 mg/mL (10.39 mM); Clear solution
This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: ≥ 2.5 mg/mL (10.39 mM); Clear solution
This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
Please enter the basic information of animal experiments:
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Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
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%DMSO +
Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
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%+
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+%Tween-80 + +
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%Saline +
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Working solution concentration: 0.22 mg/mL
Method for preparing stock solution: mg drug dissolved in μL DMSO. Stock solution concentration: mg/mL.
1. Take μL DMSO stock solution;
2. Add μL .
μL , mix evenly;
3. Then add μL Tween 80, mix evenly;
4. Then add μL
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
Purity & Documentation
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Data Sheet (272 KB)
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SDS (252 KB)
- English - EN (252 KB)
- Français - FR (252 KB)
- Deutsch - DE (252 KB)
- Norwegian - NO (252 KB)
- Español - ES (252 KB)
- Swedish - SV (252 KB)
- Italian - IT (252 KB)
- Korean - KR (252 KB)
- Portuguese - PT (252 KB)
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Handling Instructions (2659 KB)
References
[1]. Shi Y, et al. NLRP3 inflammasome inhibitor INF39 attenuated NLRP3 assembly in macrophages. Int Immunopharmacol. 2021 Mar;92:107358. [Content Brief]
[2]. Mezzaroma E,et al. NLRP3 Inflammasome Inhibitors in Cardiovascular Diseases. Molecules. 2021 Feb 12;26(4):976. [Content Brief]
[3]. occo M, et al. Electrophilic warhead-based design of compounds preventing NLRP3 inflammasome-dependent pyroptosis. J Med Chem. 2014 Dec 26;57(24):10366-82. [Content Brief]
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
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| DMSO | 1 mM | 4.1549 mL | 20.7745 mL | 41.5489 mL | 103.8724 mL |
| 5 mM | 0.8310 mL | 4.1549 mL | 8.3098 mL | 20.7745 mL | |
| 10 mM | 0.4155 mL | 2.0774 mL | 4.1549 mL | 10.3872 mL | |
| 15 mM | 0.2770 mL | 1.3850 mL | 2.7699 mL | 6.9248 mL | |
| 20 mM | 0.2077 mL | 1.0387 mL | 2.0774 mL | 5.1936 mL | |
| 25 mM | 0.1662 mL | 0.8310 mL | 1.6620 mL | 4.1549 mL | |
| 30 mM | 0.1385 mL | 0.6925 mL | 1.3850 mL | 3.4624 mL | |
| 40 mM | 0.1039 mL | 0.5194 mL | 1.0387 mL | 2.5968 mL | |
| 50 mM | 0.0831 mL | 0.4155 mL | 0.8310 mL | 2.0774 mL | |
| 60 mM | 0.0692 mL | 0.3462 mL | 0.6925 mL | 1.7312 mL | |
| 80 mM | 0.0519 mL | 0.2597 mL | 0.5194 mL | 1.2984 mL | |
| 100 mM | 0.0415 mL | 0.2077 mL | 0.4155 mL | 1.0387 mL |
INF4E Related Classifications
HY-121988 Related Classifications
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