SphK2-IN-5
SphK2-IN-5 is a selective SphK2 inhibitor with an IC50 of 0.55 μM. SphK2-IN-5 exhibits anticancer activity against colon cancer and liver cancer.
For research use only. We do not sell to patients.
- Formula: C20H15N3O5S
- Molecular Weight:409.42
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Storage:
Please store the product under the recommended conditions in the Certificate of Analysis.
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
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Biological Activity
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Chemical Information
- Protocols
- Purity & Documentation
- References
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Help & FAQs
Biological Activity
Description
IC50 & Target
[1]|
SphK2 0.55 μM (IC50) |
In Vitro
SphK2-IN-5 (Compound 6a) potently and selectively inhibits SphK2 with an IC50 of 0.55 μM; it shows negligible activity against SphK1 at a concentration of 10 μM[1].
SphK2-IN-5 (48 h) exhibits potent and selective antiproliferative activity against the HCT-116, MC38, Hepa1-6 and HepG2 tumor cell lines, and shows no activity against non-tumorigenic NIH-3T3 cells[1].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only. Further protocols information, click here.
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Cell Line:MCF-7, HCT-116, MC38, Hepa1-6, HepG2, NIH-3T3
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Concentration:Different concentrations
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Incubation Time:48 h
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Result:Showed IC50s of >100 μM, 10.43 μM, 1.45 μM, 9.57 μM, 0.23 μM, >100 μM for MCF-7, HCT-116, MC38, Hepa1-6, HepG2, NIH-3T3, respectively.
Chemical Information
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Molecular Weight 409.42
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Formula C20H15N3O5S
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SMILES
O=C(NC/1=O)SC1=C\C2=CN(CCOC3=CC=C([N+]([O-])=O)C=C3)C4=C2C=CC=C4
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
Please store the product under the recommended conditions in the Certificate of Analysis.
Protocols
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Cell Cytotoxicity Assay
Cytotoxicity assays are usually based on the assessment of cell membrane damage, which can also be indirectly detected by measuring cell viability. Detection methods include MTT assay, CKK-8 assay, LDH assay and ATP assay, etc.
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Liver Cancer Modeling
Liver cancer can be classified into primary liver cancer and secondary liver cancer. Secondary liver cancer is the metastatic liver cancer. Primary liver cancer includes hepatocellular carcinoma (HCC), intrahepatic cholangiocarcinoma (ICC) and fibrolamellar HCC, of which HCC is the most common form, accounting for approximately 90% of primary liver cancers[1]. HCC mouse models include chemical agent-induced models, transplanted tumor models, and genetic engineered models.
Purity & Documentation
References
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
SphK2-IN-5 Related Classifications
HY-184272 Related Classifications
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