2. Neuronal Signaling Autophagy PI3K/Akt/mTOR Epigenetics
  3. Autophagy CaMK AMPK
  4. STO-609 acetate

STO-609 acetate is a selective and cell-permeable inhibitor of the Ca2+/calmodulin-dependent protein kinase kinase (CaM-KK), with Ki values of 80 and 15 ng/mL for recombinant CaM-KKα and CaM-KKβ, respectively. STO-609 acetate inhibits AMP-activated protein kinase kinase (AMPKK) activity in HeLa cell lysates with an IC50 ~0.02 g/ml.

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CAS No. : 1173022-21-3

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31 Publications Citing Use of MCE STO-609 acetate

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    STO-609 acetate purchased from MedChemExpress. Usage Cited in: Nat Aging. 2025 Jun;5(6):1097-1113.  [Abstract]

    Primary WT mouse neurons were treated with exendin-4 with or without STO-609, a CaMKK2 inhibitor. STO-609 significantly attenuated the effect of exendin-4 on AMPK phosphorylation.

    STO-609 acetate purchased from MedChemExpress. Usage Cited in: Nat Metab. 2022 Sep;4(9):1185-1201.  [Abstract]

    Mouse primary hepatocytes were incubated with 13C acetate (1 mM) containing vehicle control (PBS), ORM2 (100 ng ml−1) and STO-609 (5 μM) for 6 h. STO-609 markedly abolished suppression of DNL rate by ORM2 in primary hepatocytes from C57BL/6J healthy mice.

    STO-609 acetate purchased from MedChemExpress. Usage Cited in: Nat Metab. 2022 Sep;4(9):1185-1201.  [Abstract]

    MPHs were pre-incubated with palmitic acid (200 μM) for 12 hr, and then treated with ORM2 protein (100 ng/ml) or vehicle control (PBS) for 6 h, in the presence or absence of STO-609 (5 μM). STO-609 largely blocked the inhibitory effects of ORM2 on cellular TG accumulation and lipogenic gene expression.

    STO-609 acetate purchased from MedChemExpress. Usage Cited in: Signal Transduct Target Ther. 2021 Jan 25;6(1):29.

    Cells were pretreated with STO-609 (10 μM; 1 h) followed by exposure to NM (20 μM) for an additional 24 h. STO-609 markedly inhibited NM-induced AMPK and ULK1 activation.

    STO-609 acetate purchased from MedChemExpress. Usage Cited in: Signal Transduct Target Ther. 2021 Jan 25;6(1):29.

    Cells were treated with STO-609 (10 µM; 1 h) following the addition of NM (20 µM) for another 24 h. Total cell lysates were collected and the levels of indicated proteins were detected by western blotting. STO-609 treatment markedly inhibited NM-induced ULK1 activation and autophagy induction but had no obvious effect on NM-induced pmTOR expression in keratinocytes.

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    Description

    STO-609 acetate is a selective and cell-permeable inhibitor of the Ca2+/calmodulin-dependent protein kinase kinase (CaM-KK), with Ki values of 80 and 15 ng/mL for recombinant CaM-KKα and CaM-KKβ, respectively. STO-609 acetate inhibits AMP-activated protein kinase kinase (AMPKK) activity in HeLa cell lysates with an IC50 ~0.02 g/ml.

    In Vitro

    STO-609 inhibits the activities of recombinant CaM-KKα and CaM-KKβ isoforms, with Ki values of 80 and 15 ng/mL, respectively, and also inhibits their autophosphorylation activities. STO-609 is highly selective for CaM-KK without any significant effect on the downstream CaM kinases (CaM-KI and -IV), and the IC50 value of the compound against CaM-KII is 10 μg/mL. STO-609 inhibits constitutively active CaM-KKα as well as the wild-type enzyme. In transfected HeLa cells, STO-609 suppresses the Ca2+-induced activation of CaM-KIV in a dose-dependent manner. STO-609 significantly reduces the endogenous activity of CaM-KK in SH-SY5Y neuroblastoma cells at a concentration of 1μg/mL (80% inhibitory rate)[1].

    MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

    STO-609 acetate Related Antibodies
    Molecular Weight

    374.35

    Formula

    C21H14N2O5
    CAS No.
    SMILES

    O=C(C1=C2C3=C(C4=NC5=CC=CC=C5N4C(C3=CC=C2)=O)C=C1)O.CC(O)=O
    Shipping

    Room temperature in continental US; may vary elsewhere.
    Storage

    Please store the product under the recommended conditions in the Certificate of Analysis.
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    STO-609 acetate Related Classifications

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    • Do most proteins show cross-species activity?

      Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

    Keywords:

    STO-6091173022-21-3STO609STO 609AutophagyCaMKAMPKCalmodulin-dependent protein kinasesCalmodulin-dependent kinasesAMP-activated protein kinaseInhibitorinhibitorinhibit

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