TCO-SS-amine
TCO-SS-amine is a cleavable ADC linker used in the synthesis of antibody-drug conjugates (ADCs). TCO-SS-amine is a click chemistry reagent, it contains a TCO group that can undergo an inverse electron demand Diels-Alder reaction (iEDDA) with molecules containing Tetrazine groups.
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- CAS No.: 2918774-85-1
- Formule: C13H24N2O2S2
- Masse moléculaire:304.47
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Stockage:
-20°C, protect from light
* In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)
To place orders, for customer services and technical support, please contact: MedChemExpress USA
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Activité biologique
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Chemical Information
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Solvant et solubilité
- Protocole
- Pureté et documentation
- Références
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Help & FAQs
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Activité biologique
Description
IC50 & Target
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Disulfide Cleavable Linker |
Cleavable Linker |
In Vitro
ADCs are comprised of an antibody to which is attached an ADC cytotoxin through an ADC linker[1].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only. Further protocols information, click here.
Chemical Information
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CAS No. 2918774-85-1
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Appearance Liquid
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Masse moléculaire 304.47
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Formule C13H24N2O2S2
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Color Colorless to light yellow
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SMILES
O=C(NCCSSCCN)O[C@@H]1CC/C=C([H])/CCC1
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Livraison
Room temperature in continental US; may vary elsewhere.
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Stockage
-20°C, protect from light
* In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)
Solvant et solubilité
In Vitro:
DMSO : 250 mg/mL (821.10 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Protocole
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EdU Incorporation Assay (Click Chemistry-Based DNA Synthesis Measurement)
The EdU incorporation assay measures DNA synthesis by adding the thymidine analog 5-ethynyl-2′-deoxyuridine to cells or tissues, where it is incorporated into newly synthesized DNA during S phase. Incorporated EdU is detected by copper-catalyzed azide-alkyne cycloaddition, in which a fluorescent azide covalently reacts with the ethynyl group on EdU, allowing S-phase cells to be detected by fluorescence microscopy, flow cytometry, or high-content imaging. EdU detection does not require DNA denaturation or anti-BrdU antibody access, which preserves sample structure and improves compatibility with immunostaining and multiparameter cytometry compared with BrdU-based detection. EdU can be cytotoxic in a cell-type- and exposure-dependent manner, so pulse duration, concentration, and continuous-labeling designs should be validated for each cell type.
Pureté et documentation
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Fiche technique (268 KB)
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SDS (251 KB)
- English - EN (251 KB)
- Français - FR (251 KB)
- Deutsch - DE (251 KB)
- Norwegian - NO (251 KB)
- Español - ES (251 KB)
- Swedish - SV (251 KB)
- Italian - IT (251 KB)
- Korean - KR (251 KB)
- Portuguese - PT (251 KB)
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Instruction de manipulation (2659 KB)
Références
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| DMSO | 1 mM | 3.2844 mL | 16.4220 mL | 32.8440 mL | 82.1099 mL |
| 5 mM | 0.6569 mL | 3.2844 mL | 6.5688 mL | 16.4220 mL | |
| 10 mM | 0.3284 mL | 1.6422 mL | 3.2844 mL | 8.2110 mL | |
| 15 mM | 0.2190 mL | 1.0948 mL | 2.1896 mL | 5.4740 mL | |
| 20 mM | 0.1642 mL | 0.8211 mL | 1.6422 mL | 4.1055 mL | |
| 25 mM | 0.1314 mL | 0.6569 mL | 1.3138 mL | 3.2844 mL | |
| 30 mM | 0.1095 mL | 0.5474 mL | 1.0948 mL | 2.7370 mL | |
| 40 mM | 0.0821 mL | 0.4105 mL | 0.8211 mL | 2.0527 mL | |
| 50 mM | 0.0657 mL | 0.3284 mL | 0.6569 mL | 1.6422 mL | |
| 60 mM | 0.0547 mL | 0.2737 mL | 0.5474 mL | 1.3685 mL | |
| 80 mM | 0.0411 mL | 0.2053 mL | 0.4105 mL | 1.0264 mL | |
| 100 mM | 0.0328 mL | 0.1642 mL | 0.3284 mL | 0.8211 mL |
TCO-SS-amine Related Classifications
HY-136039 Related Classifications
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