TH470
Based on 1 Customer Validation
TH470 is a highly selective LIMK1/2 (LIM kinase1/2) inhibitor (LIMK1 IC50=9.8 nM; LIMK2 IC50=13 nM), and can be used in orphan disease research.
For research use only. We do not sell to patients.
- Purity: 99.62%
- CAS No.: 2834739-51-2
- Formula: C30H31N5O5S2
- Molecular Weight:605.73
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Storage:Powder -20°C, 3 years , 4°C, 2 years ; In solvent -80°C, 6 months , -20°C, 1 month
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Biological Activity
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Chemical Information
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Solvent & Solubility
- Purity & Documentation
- References
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Help & FAQs
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Biological Activity
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LIMK1 9.8 nM (IC50) |
LIMK2 13 nM (IC50) |
TH470 (0.05-5 μM; 12 h) inhibits the growth of neurite[1].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
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Cell Line:N1E-115 cells transfected with siRNAs against FMR1 (siFMR1) or control siRNAs (siCtr)
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Concentration:0.05, 0.5, or 5 μM
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Incubation Time:12 hours
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Result:Inhibited neurite outgrowth in a dose-dependent manner.
Chemical Information
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CAS No. 2834739-51-2
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Appearance Solid
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Molecular Weight 605.73
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Formula C30H31N5O5S2
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Color White to off-white
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SMILES
CC(C(NC1=NC=C(S1)C(NCCN(C(C2=CC=C(C=C2)S(=O)(NC3=CC=CC=C3)=O)=O)CC4=CC=CC=C4)=O)=O)C
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month
Solvent & Solubility
DMSO : 100 mg/mL (165.09 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Select the appropriate dissolution method based on your experimental animal and administration route.
- For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
- To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
- The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.
Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: 2.5 mg/mL (4.13 mM); Clear solution; Need ultrasonic
This protocol yields a clear solution of 2.5 mg/mL.
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Please enter the basic information of animal experiments:
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Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
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%DMSO +
Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
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%+
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+%Tween-80 + +
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%Saline +
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Working solution concentration: 0.22 mg/mL
Method for preparing stock solution: mg drug dissolved in μL DMSO. Stock solution concentration: mg/mL.
1. Take μL DMSO stock solution;
2. Add μL .
μL , mix evenly;
3. Then add μL Tween 80, mix evenly;
4. Then add μL
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
Purity & Documentation
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Data Sheet (271 KB)
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SDS (251 KB)
- English - EN (251 KB)
- Français - FR (251 KB)
- Deutsch - DE (251 KB)
- Norwegian - NO (251 KB)
- Español - ES (251 KB)
- Swedish - SV (251 KB)
- Italian - IT (251 KB)
- Korean - KR (251 KB)
- Portuguese - PT (251 KB)
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Handling Instructions (2659 KB)
References
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
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| DMSO | 1 mM | 1.6509 mL | 8.2545 mL | 16.5090 mL | 41.2725 mL |
| 5 mM | 0.3302 mL | 1.6509 mL | 3.3018 mL | 8.2545 mL | |
| 10 mM | 0.1651 mL | 0.8255 mL | 1.6509 mL | 4.1273 mL | |
| 15 mM | 0.1101 mL | 0.5503 mL | 1.1006 mL | 2.7515 mL | |
| 20 mM | 0.0825 mL | 0.4127 mL | 0.8255 mL | 2.0636 mL | |
| 25 mM | 0.0660 mL | 0.3302 mL | 0.6604 mL | 1.6509 mL | |
| 30 mM | 0.0550 mL | 0.2752 mL | 0.5503 mL | 1.3758 mL | |
| 40 mM | 0.0413 mL | 0.2064 mL | 0.4127 mL | 1.0318 mL | |
| 50 mM | 0.0330 mL | 0.1651 mL | 0.3302 mL | 0.8255 mL | |
| 60 mM | 0.0275 mL | 0.1376 mL | 0.2752 mL | 0.6879 mL | |
| 80 mM | 0.0206 mL | 0.1032 mL | 0.2064 mL | 0.5159 mL | |
| 100 mM | 0.0165 mL | 0.0825 mL | 0.1651 mL | 0.4127 mL |
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