Thioflavin T (solution) 

Thioflavin T (solution) is a cationic Benzothiazole dye that shows enhanced fluorescence upon binding to amyloid in tissue sections. Excitation max.: ~385 nm (free) ; ~450 nm (bound) ; Emission max.: ~445 nm (free) ; ~485 nm (bound) ^[1][2][3].
Solvent and Concentration: Sterile water: 10 mM
The 1 mL volume is defined as the base specification. All larger sizes correspond to incremental volumes of this base.

Thioflavin T is a fluorescent dye that can bind to β-fold-rich structures and is often used to measure protein fibrillation. Once bound, the emission spectrum of Thioflavin T will be red-shifted and the fluorescence intensity will increase, which can also be used to detect fibril formation. Here is a reference protocol for Aβ fibrils detection^[2][3].
Guide (The following is our recommended protocol. This protocol is only a guide and should be modified according to your specific needs).
1. Prepare a mixed sample containing LMW Aβ42 (5.5 μM) , Thioflavin T solution (20 μM) , and NaCl (100 mM).
2. The mixtures were prepared in a 96-well half-area microplate. Seal the well plate and place it in a shaking incubator at 37°C for indicated time.
3. Shake for 20 seconds before testing. Set the excitation light to 450 nm and the emission light to 485 nm. Read the fluorescence value using a microplate reader.

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

318.86

C₁₇H₁₉ClN₂S

2390-54-7

Liquid (Density: 1.301 g/cm³)

Light yellow to yellow

CC1=CC=C2[N+](C)=C(C3=CC=C(N(C)C)C=C3)SC2=C1.[Cl-]

Room temperature in continental US; may vary elsewhere.

Please store the product under the recommended conditions in the Certificate of Analysis.

• [1]. Nakano H, et al. Globular-shaped Aβ oligomers have diverse mechanisms for promoting Aβ aggregations with the facilitation of fibril elongation. Neurobiol Dis. 2025 Feb;205:106775.  [Content Brief]

  [2]. Muramatsu D, et al. ALZ-801 prevents amyloid β-protein assembly and reduces cytotoxicity: A preclinical experimental study. FASEB J. 2025 Feb 15;39(3):e70382.  [Content Brief]

  [3]. Khurana R, et al. Mechanism of thioflavin T binding to amyloid fibrils. J Struct Biol. 2005 Sep;151(3):229-38.  [Content Brief]