TLR7/8/9 antagonist 2

Name: TLR7/8/9 antagonist 2
Brand: MedChemExpress
SKU: HY-153460
Rating: 4.5 (1 reviews)

Cat. No.: HY-153460 Purity: 98.96%: Data Sheet
COA

SDS
Handling Instructions
FAQs
Technical Support

TLR7/8/9 antagonist 2 is an orally active TLR7/8/9 antagonist. TLR7/8/9 antagonist 2 has inhibitory activities for HEK/hTLR7, HEK/hTLR8 and HEK/hTLR9 with IC₅₀ values of 0.011 μM, 0.029 μM and 0,052 μM, respectively.TLR7/8/9 antagonist 2 has high bioavailability in vivo.TLR7/8/9 antagonist 2 can be used for the research of auto-inflammatory diseases such as systemic lupus erythematosus or lupus nephritis.

For research use only. We do not sell to patients.

TLR7/8/9 antagonist 2 Chemical Structure

CAS No. : 2920729-91-3

Size	Stock	Quantity
5 mg	In-stock	Estimated Time of Arrival: December 31
10 mg	In-stock	Estimated Time of Arrival: December 31
25 mg	In-stock	Estimated Time of Arrival: December 31
50 mg	In-stock	Estimated Time of Arrival: December 31
100 mg	Get quote
200 mg	Get quote

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Customer Review

Based on 1 publication(s) in Google Scholar

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Biological Activity
Purity & Documentation
References
Customer Review

Description

IC₅₀ & Target

IC50: 0.011 μM (HEK/hTLR7); 0.029 μM (HEK/hTLR8); 0.052 μM (HEK/hTLR9); 420 nM (hPBMC/TLR9)^[1].

In Vitro

TLR7/8/9 antagonist 2 (example 7) has inhibitory activities for HEK/hTLR7, HEK/hTLR8 and HEK/hTLR9 with IC₅₀ values of 0.011 μM, 0.029 μM and 0,052 μM, respectively^[1].
TLR7/8/9 antagonist 2 has inhibitory activities for hPBMC/TLR9 with IC₅₀ value of 420 nM^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

In Vivo

TLR7/8/9 antagonist 2 (example 7) has high bioavailability in vivo^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model:

Male Wister-Han Rats^[1]

Dosage:

2 mg/kg, 10 mg/kg

Administration:

p.o., i.v.; single dose

Result:

PO C_max(ng/mL)	PO AUC_0-last(h*ng/mL)	IV AUC_0-last(h*ng/mL)	CL (mL/min/kg)	Vss (L/kg)	T_1/2(h)	F(%)
465	7514	1516	16	16.8	14.7	100

Molecular Weight

405.54

Formula

C₂₃H₃₁N₇

CAS No.

2920729-91-3

Appearance

Solid

Color

White to off-white

SMILES

CN1N=CC2=C(C=C(C)N=C21)N(CC3)CCC3C4=NC=C(N5CCNCC5)C=C4C

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

Powder	-20°C	3 years
	4°C	2 years
In solvent	-80°C	6 months
	-20°C	1 month

Solvent & Solubility

In Vitro:

DMSO : 100 mg/mL (246.58 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	2.4658 mL	12.3292 mL	24.6585 mL
5 mM	0.4932 mL	2.4658 mL	4.9317 mL
10 mM	0.2466 mL	1.2329 mL	2.4658 mL

View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Molarity Calculator
Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

Concentration _(start)

Volume _(start)

Concentration _(final)

Volume _(final)

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 2.5 mg/mL (6.16 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Protocol 2

Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: ≥ 2.5 mg/mL (6.16 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
Protocol 3

Add each solvent one by one: 10% DMSO 90% Corn Oil
Solubility: ≥ 2.5 mg/mL (6.16 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL Corn oil, and mix evenly.

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Please enter your animal formula composition:

DMSO +

Tween-80 +

Saline

Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.

The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).

Calculation results:

Working solution concentration: mg/mL

Method for preparing stock solution: mg drug dissolved in μL DMSO (Stock solution concentration: mg/mL).

The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).

Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.

If the continuous dosing period exceeds half a month, please choose this protocol carefully.

Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.

Purity & Documentation

Purity: 98.96%

Select Batch:

Data Sheet (274 KB) SDS (252 KB)

COA (249 KB) HNMR (145 KB) RP-HPLC (169 KB) LCMS (104 KB)

Handling Instructions (2659 KB)

References

[1]. Dongdong Chen, et al. Pyrazolo[3,4-b]pyridine compounds for the treatment of autoimmune disease. Patent WO2023046806A1.

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg

DMSO	1 mM	2.4658 mL	12.3292 mL	24.6585 mL	61.6462 mL
	5 mM	0.4932 mL	2.4658 mL	4.9317 mL	12.3292 mL
	10 mM	0.2466 mL	1.2329 mL	2.4658 mL	6.1646 mL
	15 mM	0.1644 mL	0.8219 mL	1.6439 mL	4.1097 mL
	20 mM	0.1233 mL	0.6165 mL	1.2329 mL	3.0823 mL
	25 mM	0.0986 mL	0.4932 mL	0.9863 mL	2.4658 mL
	30 mM	0.0822 mL	0.4110 mL	0.8219 mL	2.0549 mL
	40 mM	0.0616 mL	0.3082 mL	0.6165 mL	1.5412 mL
	50 mM	0.0493 mL	0.2466 mL	0.4932 mL	1.2329 mL
	60 mM	0.0411 mL	0.2055 mL	0.4110 mL	1.0274 mL
	80 mM	0.0308 mL	0.1541 mL	0.3082 mL	0.7706 mL
	100 mM	0.0247 mL	0.1233 mL	0.2466 mL	0.6165 mL