TNIK-IN-1

Cat. No.: HY-124762 Purity: 98.08%: FAQs
Data Sheet SDS COA Handling Instructions

Molarity Calculator

TNIK-IN-1 (Compound 1) is an inhibitor of Traf2 and Nck-interacting kinase (TNIK), with IC₅₀ of 65 nM, that has antitumor activity.

For research use only. We do not sell to patients.

TNIK-IN-1 Chemical Structure

CAS No. : 933886-36-3

Size	Price	Stock	Quantity
5 mg	USD 800	In-stock	Estimated Time of Arrival: December 31
10 mg	USD 1120	In-stock	Estimated Time of Arrival: December 31
25 mg	USD 1792	In-stock	Estimated Time of Arrival: December 31
50 mg	USD 2510	In-stock	Estimated Time of Arrival: December 31
100 mg		Get quote
200 mg		Get quote

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Purity & Documentation
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Description

TNIK-IN-1 (Compound 1) is an inhibitor of Traf2 and Nck-interacting kinase (TNIK), with IC₅₀ of 65 nM, that has antitumor activity^[1].

Molecular Weight

395.43

Formula

C₁₉H₁₇N₅O₃S

CAS No.

933886-36-3

Appearance

Solid

Color

Light yellow to yellow

SMILES

O=C(C1=C(NC(C2=CC=C(NC(C)=O)C=C2)=O)SC(NC3=CC=CC=C3)=N1)N

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

Powder	-20°C	3 years
	4°C	2 years
In solvent	-80°C	6 months
	-20°C	1 month

Solvent & Solubility

In Vitro:

DMSO : 100 mg/mL (252.89 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	2.5289 mL	12.6445 mL	25.2889 mL
5 mM	0.5058 mL	2.5289 mL	5.0578 mL
10 mM	0.2529 mL	1.2644 mL	2.5289 mL

View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Molarity Calculator
Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: 2.5 mg/mL (6.32 mM); Clear solution; Need ultrasonic

This protocol yields a clear solution of 2.5 mg/mL.
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Please enter your animal formula composition:

DMSO +

Tween-80 +

Saline

Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.

The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).

Calculation results:

Working solution concentration: mg/mL

Method for preparing stock solution: mg drug dissolved in μL DMSO (Stock solution concentration: mg/mL).

The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).

Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.

If the continuous dosing period exceeds half a month, please choose this protocol carefully.

Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.

Purity & Documentation

Purity: 98.08%

Select Batch:

Data Sheet (275 KB) SDS (251 KB)

COA (256 KB) HNMR (140 KB) RP-HPLC (139 KB) LCMS (355 KB)

Handling Instructions (2659 KB)

References

[1]. Koc-Kan Ho, et al. Discovery of 4-phenyl-2-phenylaminopyridine based TNIK inhibitors. Bioorg Med Chem Lett. 2013 Jan 15;23(2):569-73. [Content Brief]

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg

DMSO	1 mM	2.5289 mL	12.6445 mL	25.2889 mL	63.2223 mL
	5 mM	0.5058 mL	2.5289 mL	5.0578 mL	12.6445 mL
	10 mM	0.2529 mL	1.2644 mL	2.5289 mL	6.3222 mL
	15 mM	0.1686 mL	0.8430 mL	1.6859 mL	4.2148 mL
	20 mM	0.1264 mL	0.6322 mL	1.2644 mL	3.1611 mL
	25 mM	0.1012 mL	0.5058 mL	1.0116 mL	2.5289 mL
	30 mM	0.0843 mL	0.4215 mL	0.8430 mL	2.1074 mL
	40 mM	0.0632 mL	0.3161 mL	0.6322 mL	1.5806 mL
	50 mM	0.0506 mL	0.2529 mL	0.5058 mL	1.2644 mL
	60 mM	0.0421 mL	0.2107 mL	0.4215 mL	1.0537 mL
	80 mM	0.0316 mL	0.1581 mL	0.3161 mL	0.7903 mL
	100 mM	0.0253 mL	0.1264 mL	0.2529 mL	0.6322 mL

TNIK-IN-1 Related Classifications

Help & FAQs

Do most proteins show cross-species activity?
Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

TNIK-IN-1933886-36-3OthersTNIK InhibitorCancerInhibitorinhibitorinhibit

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