Auramine O
Based on 1 Customer Validation
Auramine O is a yellow fluorescent dye. Auramine O is used to detect amyloid fibrils. Auramine O promotes lung malignancy. Auramine O is also used to determine algal cell viability and stain acid-fast bacteria.
For research use only. We do not sell to patients.
- Purity: 83.12%
- CAS No.: 2465-27-2
- Formula: C17H22ClN3
- Molecular Weight:303.84
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Storage:
RT, sealed storage, away from moisture and light.
In solvent -80°C, 1 year , -20°C, 6 months
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
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Biological Activity
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Chemical Information
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Solvent & Solubility
- Purity & Documentation
- References
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Help & FAQs
Biological Activity
Guidelines (Following is our recommended protocol. This protocol only provides a guideline, and should be modified according to your specific needs).
Auramine O staining of Tuberculosis bacilli[3]
1. Place the slide on a staining rack and stain with Auramine O solution for 20 min
2. Rinse the slide with water and soak it in 0.5% acid alcohol for 3 min
3. Rinse with water and counterstain with 1% KMnO4 solution for 1 min
4. Check the staining effect with an LED microscope with at least 40 fields of view
Note: Tuberculosis bacilli appear as yellow luminescent organisms in a dark field of view
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
| NCT Number | Sponsor | Condition | Start Date |
Phase
|
|---|---|---|---|---|
| NCT01329991 | Plexxikon| | 2011-05 | PHASE1 |
Chemical Information
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CAS No. 2465-27-2
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Appearance Solid
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Molecular Weight 303.84
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Formula C17H22ClN3
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Color Light yellow to yellow
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SMILES
N=C(C1=CC=C(N(C)C)C=C1)C2=CC=C(N(C)C)C=C2.[H]Cl
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
RT, sealed storage, away from moisture and light
In solvent -80°C 1 year -20°C 6 months
Solvent & Solubility
DMSO : 16.67 mg/mL (54.86 mM; ultrasonic and warming and heat to 60°C; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 1 year; -20°C, 6 months. When stored at -80°C, please use it within 1 year. When stored at -20°C, please use it within 6 months.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 1 year; -20°C, 6 months. When stored at -80°C, please use it within 1 year. When stored at -20°C, please use it within 6 months.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Purity & Documentation
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Data Sheet (283 KB)
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SDS (761 KB)
- English - EN (761 KB)
- Français - FR (761 KB)
- Deutsch - DE (761 KB)
- Norwegian - NO (761 KB)
- Español - ES (761 KB)
- Swedish - SV (761 KB)
- Italian - IT (761 KB)
- Korean - KR (761 KB)
- Portuguese - PT (761 KB)
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Handling Instructions (2659 KB)
References
[1]. Kommareddi S, et al. Nontuberculous mycobacterial infections: comparison of the fluorescent auramine-O and Ziehl-Neelsen techniques in tissue diagnosis. Hum Pathol. 1984 Nov;15(11):1085-9. [Content Brief]
[3]. Gizaw N, et al. The yield of Auramine O staining using led microscopy with bleach treated sputum samples for detection of pulmonary tuberculosis at St. Peter tuberculosis specialized hospital, Addis Ababa, Ethiopia. J Clin Tuberc Other Mycobact Dis. 2019 Dec 12;18:100140. [Content Brief]
[4]. Amdursky N, et al. Auramine-O as a fluorescence marker for the detection of amyloid fibrils. J Phys Chem B. 2012 Nov 15;116(45):13389-95. [Content Brief]
[5]. Tung JC, et al. Auramine O, an incense smoke ingredient, promotes lung cancer malignancy. Environ Toxicol. 2017 Nov;32(11):2379-2391. [Content Brief]
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 1 year; -20°C, 6 months. When stored at -80°C, please use it within 1 year. When stored at -20°C, please use it within 6 months.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| DMSO | 1 mM | 3.2912 mL | 16.4560 mL | 32.9121 mL | 82.2801 mL |
| 5 mM | 0.6582 mL | 3.2912 mL | 6.5824 mL | 16.4560 mL | |
| 10 mM | 0.3291 mL | 1.6456 mL | 3.2912 mL | 8.2280 mL | |
| 15 mM | 0.2194 mL | 1.0971 mL | 2.1941 mL | 5.4853 mL | |
| 20 mM | 0.1646 mL | 0.8228 mL | 1.6456 mL | 4.1140 mL | |
| 25 mM | 0.1316 mL | 0.6582 mL | 1.3165 mL | 3.2912 mL | |
| 30 mM | 0.1097 mL | 0.5485 mL | 1.0971 mL | 2.7427 mL | |
| 40 mM | 0.0823 mL | 0.4114 mL | 0.8228 mL | 2.0570 mL | |
| 50 mM | 0.0658 mL | 0.3291 mL | 0.6582 mL | 1.6456 mL |
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