Ganoderic acid AM1
Ganoderic acid AM1 (GAAM1) is a natural product that can be found in ganoderma lucidum. Ganoderic acid AM1 shows cytotoxicity with an IC50 value of 19.8 µM for HeLa cells.
For research use only. We do not sell to patients.
- CAS No.: 149507-55-1
- Formula: C30H42O7
- Molecular Weight:514.65
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Storage:
Please store the product under the recommended conditions in the Certificate of Analysis.
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Biological Activity
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Chemical Information
- Protocols
- Purity & Documentation
- References
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Help & FAQs
Biological Activity
Description
Cellular Effect
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Cell Line
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Type | Value | Description | References |
|---|---|---|---|---|
| Bel-7402 | IC50 |
7.25 μM
Compound: 17
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Cytotoxicity against human Bel7402 cells
Cytotoxicity against human Bel7402 cells
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[PMID: 23092389] |
| HeLa | IC50 |
202 μM
Compound: 17
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Cytotoxicity against human HeLa cells
Cytotoxicity against human HeLa cells
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[PMID: 23092389] |
| P388 | IC50 |
7.25 μM
Compound: 17
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Cytotoxicity against mouse P388 cells
Cytotoxicity against mouse P388 cells
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[PMID: 23092389] |
| SGC-7901 | IC50 |
7.25 μM
Compound: 17
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Cytotoxicity against human SGC7901 cells
Cytotoxicity against human SGC7901 cells
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[PMID: 23092389] |
Chemical Information
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CAS No. 149507-55-1
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Molecular Weight 514.65
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Formula C30H42O7
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SMILES
CC(C)([C@](C1)2[H])[C@@H](O)CC[C@]2(C)C3=C([C@@]4(C(C[C@@H]([C@]4(CC3=O)C)[C@H](C)CC(CC(C)C(O)=O)=O)=O)C)C1=O
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Synonyms
GAAM1
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Structure Classification
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Initial Source
Ganoderma lucidum (Curtis) P. Karst.
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
Please store the product under the recommended conditions in the Certificate of Analysis.
Protocols
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Cell Cytotoxicity Assay
Cytotoxicity assays are usually based on the assessment of cell membrane damage, which can also be indirectly detected by measuring cell viability. Detection methods include MTT assay, CKK-8 assay, LDH assay and ATP assay, etc.
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Mammalian live/dead viability and cytotoxicity staining
Live/dead viability and cytotoxicity staining assays are based on the simultaneous detection of intracellular esterase activity in metabolically active (viable) cells and membrane integrity loss in non-viable cells. In commonly used dual-staining approaches, membrane-permeant fluorogenic substrates are converted by intracellular esterases into fluorescent products in live cells, while impermeant DNA-binding dyes selectively enter cells with compromised plasma membranes and label nucleic acids in dead or dying cells, enabling discrimination between viable and non-viable populations by fluorescence microscopy or flow cytometry.
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Cell Viability Determination by MTT Colorimetric Assay
The following protocol uses the MTT colorimetric assay as a classic literature-established method for assessing cell viability/metabolic activity in cultured mammalian cells. MTT[3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide] is reduced by metabolically active cells to a colored formazan product; the amount of formazan is quantified spectrophotometrically and provides an indirect measure of metabolically active viable cells. Importantly, MTT reduction reflects cellular oxidoreductase/metabolic activity rather than an absolute direct count of living cells, so changes in cellular metabolism can alter the signal independently of cell number.
Purity & Documentation
References
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Ganoderic acid AM1 Related Classifications
HY-129148 Related Classifications
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