RAD16-I

The self-assembling peptide hydrogel RAD16-I (0.1-0.3% w/v; up to 21 days) maintains high viability of subATDPCs for up to 21 days, supports basal upregulation of cardiac markers in control medium, enhances the upregulation of cardiac markers when combined with cardiac induction medium, and regulates the viscoelasticity of the scaffold over time^[1].
Modified RAD16-I self-assembling peptide hydrogels (0.15-0.3% w/v) bearing the RGD motif, heparin, or both maintain high viability of subATDPCs, promote cell elongation and orderly arrangement; compared with unmodified RAD16-I hydrogels, they do not significantly alter the expression of myocardial markers; while under cardiomyogenic induction conditions, RAD16-I/RADRGD hydrogels with enhanced initial stiffness downregulate the expression of myocardial proteins in subATDPCs^[1].
RAD16-I self-assembles into β-sheet-containing nanofibers under physiological conditions, with a diameter of 19.58 nm^[3].
The 3D sandwich culture system with RAD16-I maintains the long-term viability of dedifferentiated human ICCs (with a cell survival rate of approximately 85% on day 18 of culture) and also promotes the aggregation of ICCs into larger cell clusters within 18 days, but does not significantly upregulate the expression of β-cell-specific genes compared with the non-adherent control group^[3].
RAD16-I can adsorb onto poly (ethyl acrylate), 90/10 ethyl acrylate-acrylic acid copolymer (mass ratio), and glass coverslip substrates, forming β-sheet structures detectable by Congo Red staining, while unremoved excess peptides form a three-dimensional gel layer with weak adhesion^[4].
RAD16-I (0.025%; 1 h at 37°C) can adsorb and form unique structures on the surfaces of polyethyl acrylate, 90/10 wt% ethyl acrylate-acrylic acid copolymer, and glass coverslip substrates under the conditions of Protocol 2 (no induced self-assembly); among them, the coating supported by 90/10 wt% ethyl acrylate-acrylic acid copolymer is more uniform than that supported by polyethyl acrylate, and the root-mean-square roughness values of the three at a concentration of 0.025% are 0.54 nm, 0.15 nm, and 1.24 nm, respectively^[4].
Adsorption of RAD16-I (0.025-0.1%; 1 h at 37°C, 4 h self-assembly) alters the surface wettability of polyethyl acrylate, ethyl acrylate-acrylic acid copolymer with a mass ratio of 90/10, and glass coverslip substrates, while inducing substrate-specific changes in contact angles and polar components of surface tension; in addition, pre-swelled substrates show a better correlation between wettability and peptide coating uniformity observed by AFM^[4].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.