Cat. No.: HY-148266

UBA5-IN-1

2 Cited Publications

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Based on 2 publication(s) in Google Scholar

UBA5-IN-1 (compound 8.5) is a selective UBA5 inhibitor with an IC₅₀ value of 4.0 μM. UBA5-IN-1 inhibits cell proliferation of Sk-Luci6 cancer cells with high expression levels of UBA5.

For research use only. We do not sell to patients.

Purity: 98.0%
CAS No.: 1831169-11-9
Formula: C₂₆H₄₀F₆N₁₀O₁₁S₂Zn
Molecular Weight:912.16

Storage:

Powder -20°C, 3 years , 4°C, 2 years ; In solvent -80°C, 6 months , -20°C, 1 month

UBA5-IN-1 Chemical Structure

CAS No.: 1831169-11-9

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Fluorescent Dye

Strong Fluorescence Signal / Multi-Disciplinary Applications / High Fluorescence Specificity

Biological Activity
- Description
- IC₅₀ & Target
- In Vitro
Chemical Information
Publications (2)
Solvent & Solubility
- In Vitro
- In Vivo
Purity & Documentation
References
Help & FAQs
Classifications
Bioz

Biological Activity

Description

UBA5-IN-1 (compound 8.5) is a selective UBA5 inhibitor with an IC₅₀ value of 4.0 μM. UBA5-IN-1 inhibits cell proliferation of Sk-Luci6 cancer cells with high expression levels of UBA5^[1].

IC₅₀ & Target

IC50: 4.0 μM (UBA5), 78.5 μM (UAE), 66.8 μM (NAE)^[1]

In Vitro

UBA5-IN-1 (0.1-1000 μM) shows dose-dependent inhibition effect to UBA5 with an IC₅₀ value of 4.0 μM, and inhibits UAE and NAE with IC₅₀ values of 78.5 and 66.8 μM, respectively^[1].
UBA5-IN-1 (0-5 μM) inhibits UBA5 non-competitively with respect to ATP^[1].
UBA5-IN-1 (10 μM) shows no effect on ATP binding to human kinase^[1].
UBA5-IN-1 (0-50 μM; 72 h) effects cell proliferation of Sk-Luci6 cancer cells with high expression levels of UBA5^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Cell Proliferation Assay^[1]

Cell Line:

Sk-Luci6 cancer cells with high expression levels of UBA5
Concentration:

0-50 μM
Incubation Time:

0-72 h
Result:

Showed selective anti-proliferative activity for Sk-Luci6 cancer cells which expressed higher UBA5 protein levels, but showed no anti-proliferative activity to MRC9 lung fibroblasts and A549 carcinoma cells.

Chemical Information

CAS No. 1831169-11-9
Appearance Solid
Molecular Weight 912.16
Formula C₂₆H₄₀F₆N₁₀O₁₁S₂Zn
Color White to off-white
SMILES

O=S(C(F)(F)F)([O-])=O.O=S(C(F)(F)F)([O-])=O.NC1=C(N=CN2[C@H]3[C@H](O)[C@H](O)[C@@H](C(NCCCCCC([N]45[Zn+2]67[N@@](CC[N]6([H])CC[N@]7([H])CC5)([H])CC4)=O)=O)O3)C2=NC=N1
Shipping

Room temperature in continental US; may vary elsewhere.

Storage

Powder	-20°C	3 years
	4°C	2 years
In solvent	-80°C	6 months
	-20°C	1 month

Publications (2)

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Journal Impact Factor

Proc Natl Acad Sci U S A

UFMylation: A supervisor of the HIF1α pathway and a potential therapeutic target for anti-PD-1 combination therapy in hypoxic tumors. [Abstract]

2025 Jul 8;122(27):e2500562122. PMID: 40601633
iScience

UFMylation maintains YAP stability to promote vascular endothelial cell senescence. [Abstract]

2025 Jan 21;28(2):111854. PMID: 39991547

Solvent & Solubility

In Vitro:

DMF : 100 mg/mL (109.63 mM; Need ultrasonic)

H₂O : 50 mg/mL (54.81 mM; Need ultrasonic)

DMSO : 50 mg/mL (54.81 mM; ultrasonic and warming and heat to 60°C; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

View the Complete Stock Solution Preparation Table

Preparing Stock Solutions

View the Complete Stock Solution Preparation Table

Concentration Solvent Mass

1 mg

5 mg

10 mg

Concentration

Solvent

Mass

1 mM

1.0963 mL

5.4815 mL

10.9630 mL

5 mM

0.2193 mL

1.0963 mL

2.1926 mL

10 mM

0.1096 mL

0.5481 mL

1.0963 mL

Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.

Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

Molarity Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

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Dilution Calculator

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In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

Stock Solution Working Solution

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline