UH15-38
Based on 2 publication(s) in Google Scholar
UH15-38 is a potent RIPK3 inhibitor with an IC50 value of 20 nM. UH15-38 blocks IAV (influenza A virus)-activated necroptosis. UH15-38 dampens IAV-induced lung injury.
Nur für Forschungszwecke. Wir verkaufen nicht an Patienten.
- Reinheit : 99.80%
- CAS. Nr.: 2540881-21-6
- Formel: C26H27N5O2
- Molecular Weight:441.52
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Speicherung:Powder -20°C, 3 years , 4°C, 2 years ; In solvent -80°C, 6 months , -20°C, 1 month
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
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Biologische Aktivität
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Chemical Information
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Lösungsmittel & Löslichkeit
- Protokoll
- Reinheit & Dokumentation
- Verweise
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Help & FAQs
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GSK2982772 (Standard)Reference Standard
Publications Citing Use of MedChemExpress (MCE) UH15-38
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Customer Validation & Images
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Cell Proliferation/Viability Assay
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RT-PCR
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WB
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IF
Biologische Aktivität
Beschreibung
IC50 & Target
[1]|
RIPK3 20 nM (IC50) |
In Vitro
UH15-38 (0-1000 nM; 12 h) decreases the expression of pMLKL induced by TCZ in a dose-dependent manner[1].
UH15-38 (0-1000 nM) protects type I alveolar epithelial cells from canonical TNF-induced necroptosis and prevents IAV-induced necroptosis[1].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only. Further protocols information, click here.
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Cell Line:Mouse embryonic fibroblasts
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Concentration:0, 25, 50, 100, 250, 500, 750, 1000 nM
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Incubation Time:12 h
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Result:Decreased the expression of pMLKL induced by TCZ (mouse TNF (100 ng/ml), cycloheximide (250 ng/ml) and zVAD (50 μM)) in a time dependent manner.
In Vivo
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
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Animal Model:C57Bl/6 mice (lethal dose of PR8 (6,000 × EID50))[1]
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Dosage:30 mg/kg
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Administration:I.p.; once daily for 4 days; starting 24 h after infection
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Result:Markedly reduced numbers of pMLKL+ necroptotic cells, decreased in diffuse alveolar damage and the formation of hyaline membranes, reduced the extent of fibrotic lung damage, displayed significantly lower levels of the pro-fibrotic mediators (IL-1β, IL-6, IL-18, IL-17 and CCL5).
Chemical Information
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CAS. Nr. 2540881-21-6
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Appearance Solid
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Molecular Weight 441.52
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Formel C26H27N5O2
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Color White to light yellow
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SMILES
CN1CCN(CC1)C2=CC(NC3=NC=C4C=C(C(N(C4=C3)C)=O)C5=CC(O)=CC=C5)=CC=C2
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Versand
Room temperature in continental US; may vary elsewhere.
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Speicherung
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month
Publications (2)
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Journal Impact Factor
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Most Recent
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Transbound Emerg Dis
Activation of ZBP1/RIPK3/MLKL-Dependent Necroptosis by Pseudorabies Virus Restricts Viral Infection in BV2 Microglia Cells. [Abstract]2025 Oct 7:2025:8510846. PMID: 41098523
UH15-38 purchased from MedChemExpress. Usage Cited in: Transbound Emerg Dis. 2025 Oct 7:2025:8510846. [Abstract]
Cell viability of PRV-infected BV2 microglial cells treated with UH15-38 (0.01, 0.1, 1 μM).
UH15-38 purchased from MedChemExpress. Usage Cited in: Transbound Emerg Dis. 2025 Oct 7:2025:8510846. [Abstract]
BV2 microglial cells were pretreated with UH15-38 (0.01, 0.1, 0.5 μM) for 1 h and then infected with PRV for 24 h. The IFN-β, ISG15, and Mx2 mRNA levels were measured by qRT-PCR.
UH15-38 purchased from MedChemExpress. Usage Cited in: Transbound Emerg Dis. 2025 Oct 7:2025:8510846. [Abstract]
BV2 microglial cells were pretreated with UH15-38 (0.01, 0.1, and 0.5 μM) for 1 h, and then PRV JS-2012 was used to infect BV2 microglial cells for 24 h. Then, PRV gE expression levels were analyzed by western blot.
UH15-38 purchased from MedChemExpress. Usage Cited in: Transbound Emerg Dis. 2025 Oct 7:2025:8510846. [Abstract]
BV2 microglial cells were pretreated with UH15-38 (0.01, 0.1, and 0.5 μM) for 1 h, and then PRV JS-2012 was used to infect BV2 microglial cells for 24 h. The expression of PRV gE and p-RIPK3 was visualized by IFA.
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Lösungsmittel & Löslichkeit
In Vitro:
DMSO : 100 mg/mL (226.49 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Konzentration (Stammlösung) × Volumen (Stammlösung) = Konzentration (Ziellösung) × Volumen (Ziellösung)
In Vivo:
Select the appropriate dissolution method based on your experimental animal and administration route.
- For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
- To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
- The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.
Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 5 mg/mL (11.32 mM); Clear solution
This protocol yields a clear solution of ≥ 5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (50.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: ≥ 5 mg/mL (11.32 mM); Clear solution
This protocol yields a clear solution of ≥ 5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (50.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:
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Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
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%DMSO +
Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
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%+
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+%Tween-80 + +
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%Saline +
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Working solution concentration: 0.22 mg/mL
Method for preparing stock solution: mg drug dissolved in μL DMSO. Stock solution concentration: mg/mL.
1. Take μL DMSO stock solution;
2. Add μL .
μL , mix evenly;
3. Then add μL Tween 80, mix evenly;
4. Then add μL
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
Protokoll
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Cell Cytotoxicity Assay
Cytotoxicity assays are usually based on the assessment of cell membrane damage, which can also be indirectly detected by measuring cell viability. Detection methods include MTT assay, CKK-8 assay, LDH assay and ATP assay, etc.
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Inhalation Toxicity Study
Inhalation toxicity studies expose rodents to a controlled aerosol, vapor, gas, or smoke atmosphere and assess respiratory and systemic toxicity using exposure-atmosphere characterization, clinical observations, body and organ weights, bronchoalveolar lavage fluid, histopathology, blood chemistry, hematology, and, when included, molecular endpoints such as transcriptomics, proteomics, lipidomics, or tissue burden analysis. The primary biological readouts are airway irritation, pulmonary inflammation, cytotoxicity, altered surfactant or lipid homeostasis, impaired particle clearance, and tissue remodeling, reflected by BALF cell differentials, BALF protein, LDH, phosphatase activities, cytokines, lung weight, microscopic respiratory-tract lesions, and retained lung burden.
Reinheit & Dokumentation
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Data Sheet (273 KB)
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SDS (254 KB)
- English - EN (254 KB)
- Français - FR (254 KB)
- Deutsch - DE (254 KB)
- Norwegian - NO (254 KB)
- Español - ES (254 KB)
- Swedish - SV (254 KB)
- Italian - IT (254 KB)
- Korean - KR (254 KB)
- Portuguese - PT (254 KB)
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Handling Instructions (2659 KB)
Verweise
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
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| DMSO | 1 mM | 2.2649 mL | 11.3245 mL | 22.6490 mL | 56.6226 mL |
| 5 mM | 0.4530 mL | 2.2649 mL | 4.5298 mL | 11.3245 mL | |
| 10 mM | 0.2265 mL | 1.1325 mL | 2.2649 mL | 5.6623 mL | |
| 15 mM | 0.1510 mL | 0.7550 mL | 1.5099 mL | 3.7748 mL | |
| 20 mM | 0.1132 mL | 0.5662 mL | 1.1325 mL | 2.8311 mL | |
| 25 mM | 0.0906 mL | 0.4530 mL | 0.9060 mL | 2.2649 mL | |
| 30 mM | 0.0755 mL | 0.3775 mL | 0.7550 mL | 1.8874 mL | |
| 40 mM | 0.0566 mL | 0.2831 mL | 0.5662 mL | 1.4156 mL | |
| 50 mM | 0.0453 mL | 0.2265 mL | 0.4530 mL | 1.1325 mL | |
| 60 mM | 0.0377 mL | 0.1887 mL | 0.3775 mL | 0.9437 mL | |
| 80 mM | 0.0283 mL | 0.1416 mL | 0.2831 mL | 0.7078 mL | |
| 100 mM | 0.0226 mL | 0.1132 mL | 0.2265 mL | 0.5662 mL |
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