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Cat. No.: HY-177783

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iDeg-1

iDeg-1 is a potent IDO1 molecular glue degrader. iDeg-1 binds to apo-IDO1 and alters its C-terminal conformation, promoting CRL2-KLHDC3 E3 ligase-mediated ubiquitination and degradation of IDO1. iDeg-1 can be used in cancer-related research.

For research use only. We do not sell to patients.

Formula: C₃₀H₄₀N₂O₄S
Molecular Weight:524.71

Storage:

Please store the product under the recommended conditions in the Certificate of Analysis.

Data Sheet (288 KB)

iDeg-1 Chemical Structure

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Use of MCE Products

Strong Fluorescence Signal / Multi-Disciplinary Applications / High Fluorescence Specificity

Biological Activity
- Description
- IC₅₀ & Target
- Cellular Effect
- In Vitro
Chemical Information
Purity & Documentation
References
Calculators
Help & FAQs
Classifications
Bioz

Biological Activity

Description

IC₅₀ & Target

^[1]

IDO1

All Indoleamine 2,3-Dioxygenase (IDO) Isoforms :

Cellular Effect

Cell Line	Type	Value	Description	References
BXPC-3	IC₅₀	1.1 μM	Inhibition of kynurenine (Kyn) formation in IFN-γ-stimulated human BxPC3 cells incubated for 48 h, quantified using p-DMAB. Inhibition of kynurenine (Kyn) formation in IFN-γ-stimulated human BxPC3 cells incubated for 48 h, quantified using p-DMAB.	41501556
SK-OV-3	IC₅₀	1.6 μM	Inhibition of kynurenine (Kyn) formation in IFN-γ-stimulated human SKOV-3 cells incubated for 48 h, quantified using p-DMAB. Inhibition of kynurenine (Kyn) formation in IFN-γ-stimulated human SKOV-3 cells incubated for 48 h, quantified using p-DMAB.	41501556
HeLa	IC₅₀	1.7 μM	Inhibition of kynurenine (Kyn) formation in IFN-γ-stimulated human HeLa cells incubated for 48 h, quantified using p-DMAB. Inhibition of kynurenine (Kyn) formation in IFN-γ-stimulated human HeLa cells incubated for 48 h, quantified using p-DMAB.	41501556
HEK-293T	IC₅₀	0.45 μM	Inhibition of kynurenine (Kyn) formation in recombinant human IDO1 (rhIDO1)-electroporated human HEK293T cells. Inhibition of kynurenine (Kyn) formation in recombinant human IDO1 (rhIDO1)-electroporated human HEK293T cells.	41501556
HEK-293T	IC₅₀	0.42 μM	Inhibition of kynurenine (Kyn) formation in transiently IDO1-expressing human HEK293T cells. Inhibition of kynurenine (Kyn) formation in transiently IDO1-expressing human HEK293T cells.	41501556
BXPC-3	IC₅₀	0.83 μM	Inhibition of kynurenine formation via indoleamine-2,3-dioxygenase 1 enzymatic activity in IFN-γ-stimulated BxPC3 cells incubated for 48 hrs by Kyn assay. Inhibition of kynurenine formation via indoleamine-2,3-dioxygenase 1 enzymatic activity in IFN-γ-stimulated BxPC3 cells incubated for 48 hrs by Kyn assay.	41501556

In Vitro

iDeg-1 (48 h) inhibits the production of kynurenine (Kyn) in BxPC3, SKOV-3, and HeLa cells stimulated with IFN-γ (HY-P7025), with IC₅₀ values of 1.1 μM, 1.6 μM, and 1.7 μM, respectively^[1].
iDeg-1 (0.03-50 μM; 6-24 h) reduces IDO1 protein levels and induces polyubiquitination of IDO1 in IFN-γ-stimulated BxPC3 cells in a dose-dependent manner^[1].
iDeg-1 reduces IDO1 protein levels in a time-dependent manner and decreases Kyn production in a dose-dependent manner in HEK293T cells (electroporated with rhIDO1 or transfected with plasmids), with IC₅₀ values of approximately 0.45 μM and 0.42 μM, respectively^[1].

iDeg-1 (50 μM; 1 h) binds to and thermally stabilizes the IDO1 protein in SKOV-3 cells (ΔT_m = 3.5 °C)^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Western Blot Analysis^[1]

Cell Line:

BxPC3
Concentration:

0.03 μM, 0.12 μM, 0.37 μM, 1.11 μM, 3.33 μM, 10 μM
Incubation Time:

24 h
Result:

Reduced endogenous IDO1 protein levels in a dose-dependent manner.

Western Blot Analysis^[1]

Cell Line:

BxPC3
Concentration:

50 μM
Incubation Time:

6 h
Result:

Markedly increased the polyubiquitination levels of IDO1.

Chemical Information

Molecular Weight 524.71
Formula C₃₀H₄₀N₂O₄S
SMILES

CC1=CC=C(C=C1)NC(OC[C@@]23[C@H](C[C@@H]4C[C@]2([H])CN(C3)S(C5=CC=C(C(C)(C)C)C=C5)(=O)=O)C4(C)C)=O
Shipping

Room temperature in continental US; may vary elsewhere.
Storage

Please store the product under the recommended conditions in the Certificate of Analysis.

Purity & Documentation

Select Batch:

Purity: 99.98%

Assay: 99.98%

ee.: 99.98%

Data Sheet (288 KB)
COA (2659 KB)
Elemental Analysis Report (2659 KB)
HNMR (2659 KB)
CNMR (2659 KB)
RP-HPLC (2659 KB)
NP-HPLC (2659 KB)
MS (2659 KB)
LCMS (2659 KB)
IR (2659 KB)
GC (2659 KB)
OR (2659 KB)
SDS-PAGE (2659 KB)
SEC-HPLC (2659 KB)
Other Report (2659 KB)
Handling Instructions (2659 KB)

References

[1]. Hennes E, et al. Monovalent pseudo-natural products supercharge degradation of IDO1 by its native E3 KLHDC3. Nature chemistry. 2026 Mar;18(3):585-596.

Calculators

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Help & FAQs

How should lyophilized recombinant proteins be reconstituted and stored?

1. Before opening the cap, centrifuge the vial at 13000 rpm for 20-30 seconds. This step will ensure that any lyophilized powder that may have adhered to the cap or walls is collected at the bottom of the vial, minimizing the risk of product loss.

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100 mg

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