FA-2
Based on 1 Customer Validation
FA-2, a hydrogenated derivative of WU-FA-00, is an antibacterial agent that exhibits high levels of antibacterial activity against Gram-positive strains and also has some anti-inflammatory activity.
For research use only. We do not sell to patients.
- Purity: 95.0%
- CAS No.: 882429-53-0
- Formula: C34H52O9
- Molecular Weight:604.77
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Storage:Powder -20°C, 3 years , 4°C, 2 years ; In solvent -80°C, 6 months , -20°C, 1 month
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Biological Activity
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Chemical Information
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Solvent & Solubility
- Purity & Documentation
- References
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Help & FAQs
Biological Activity
FA-2 (0-25 µg/mL, 24 h) has a dose-dependent inhibitory effect on Gram-positive bacteria, but has no inhibitory effect on Gram-negative strains[1].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
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Animal Model:TPA-induced skin inflammation in female Kunming mice[1]
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Dosage:2000, 4000 and 8000 µg/mL
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Administration:20 µL of acetone-loaded agent topically applied to the right ear
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Result:Significantly reduced TPA-induced ear edema by 48.16%, 113.97% and 137.32%, respectively, at concentrations of 2000, 4000 and 8000 µg/mL.
Chemical Information
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CAS No. 882429-53-0
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Appearance Solid
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Molecular Weight 604.77
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Formula C34H52O9
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Color White to off-white
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SMILES
C[C@@]12[C@@]3([C@](C[C@H]([C@@]1([H])[C@@]4([C@@]([C@@H]([C@@H](CC4)O)C)([H])CC2)C)O)([H])/C([C@H](C3)OC(C)=O)=C(CCC=O)/C(OCOC(C(C)(C)C)=O)=O)C
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month
Solvent & Solubility
DMSO : 100 mg/mL (165.35 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Select the appropriate dissolution method based on your experimental animal and administration route.
- For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
- To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
- The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.
Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 2.5 mg/mL (4.13 mM); Clear solution
This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Please enter the basic information of animal experiments:
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Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
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%DMSO +
Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
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%+
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+%Tween-80 + +
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%Saline +
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Working solution concentration: 0.22 mg/mL
Method for preparing stock solution: mg drug dissolved in μL DMSO. Stock solution concentration: mg/mL.
1. Take μL DMSO stock solution;
2. Add μL .
μL , mix evenly;
3. Then add μL Tween 80, mix evenly;
4. Then add μL
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
Purity & Documentation
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Data Sheet (275 KB)
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SDS (251 KB)
- English - EN (251 KB)
- Français - FR (251 KB)
- Deutsch - DE (251 KB)
- Norwegian - NO (251 KB)
- Español - ES (251 KB)
- Swedish - SV (251 KB)
- Italian - IT (251 KB)
- Portuguese - PT (251 KB)
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Handling Instructions (2659 KB)
References
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| DMSO | 1 mM | 1.6535 mL | 8.2676 mL | 16.5352 mL | 41.3380 mL |
| 5 mM | 0.3307 mL | 1.6535 mL | 3.3070 mL | 8.2676 mL | |
| 10 mM | 0.1654 mL | 0.8268 mL | 1.6535 mL | 4.1338 mL | |
| 15 mM | 0.1102 mL | 0.5512 mL | 1.1023 mL | 2.7559 mL | |
| 20 mM | 0.0827 mL | 0.4134 mL | 0.8268 mL | 2.0669 mL | |
| 25 mM | 0.0661 mL | 0.3307 mL | 0.6614 mL | 1.6535 mL | |
| 30 mM | 0.0551 mL | 0.2756 mL | 0.5512 mL | 1.3779 mL | |
| 40 mM | 0.0413 mL | 0.2067 mL | 0.4134 mL | 1.0335 mL | |
| 50 mM | 0.0331 mL | 0.1654 mL | 0.3307 mL | 0.8268 mL | |
| 60 mM | 0.0276 mL | 0.1378 mL | 0.2756 mL | 0.6890 mL | |
| 80 mM | 0.0207 mL | 0.1033 mL | 0.2067 mL | 0.5167 mL | |
| 100 mM | 0.0165 mL | 0.0827 mL | 0.1654 mL | 0.4134 mL |
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