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  3. ZnAF-1F

ZnAF-1F is a potent fluorophore for with an Kd value of 2.2 nM. ZnAF-1F can be used as fluorescent probes for Zn2+ in cells. ZnAF-1F shows λ excitation of 489 nm and λ emission of 514 nm.

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ZnAF-1F Chemical Structure

ZnAF-1F Chemical Structure

CAS No. : 443302-08-7

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Description

ZnAF-1F is a potent fluorophore for with an Kd value of 2.2 nM. ZnAF-1F can be used as fluorescent probes for Zn2+ in cells. ZnAF-1F shows λ excitation of 489 nm and λ emission of 514 nm[1][2][3].

In Vitro

ZnAF-1F (1 μM) shows an Kon value of 3.5*106 M-1s-1, and an Koff value of 7.7*10-3 s-1 in 100mM HEPES buffer[1].
ZnAF-1F is a Zn sensors in neutral and slightly acidic conditions[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Molecular Weight

608.59

Formula

C34H26F2N4O5

CAS No.
Appearance

Solid

Color

Orange to red

Emission (Em)

514

Excitation (Ex)

489

SMILES

O=C1OC2(C3=C(OC4=C2C=C(F)C(O)=C4)C=C(O)C(F)=C3)C5=C1C=C(NCCN(CC6=NC=CC=C6)CC7=NC=CC=C7)C=C5

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

-20°C, protect from light

*In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)

Purity & Documentation

Purity: ≥92.0%

References
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ZnAF-1F Related Classifications

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    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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ZnAF-1F
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HY-D0159
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