5-Azidomethyl-2'-deoxyuridine
Based on 1 Customer Validation
5-Azidomethyl-2'-deoxyuridine (5-AmdU) is a purine nucleoside analog. 5-Azidomethyl-2'-deoxyuridine demonstrates effective radiosensitization in EMT6 tumor cells.. 5-Azidomethyl-2'-deoxyuridine is a click chemistry reagent, it contains an Azide group and can undergo copper-catalyzed azide-alkyne cycloaddition reaction (CuAAc) with molecules containing Alkyne groups. It can also undergo strain-promoted alkyne-azide cycloaddition (SPAAC) reactions with molecules containing DBCO or BCN groups.
연구목적의 판매만을 진행합니다. 환자를 대상으로 한 판매는 하지 않습니다.
- Purity : 99.76%
- CAS No.: 59090-48-1
- 화학식: C10H13N5O5
- 분자량:283.24
-
보관:
-20°C, sealed storage, away from moisture
* In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
-
Biological Activity
-
Chemical Information
-
용액&용해도
- Protocol
- 순도&문서
- References
-
Help & FAQs
Biological Activity
제품 설명
Cellular Effect
|
Cell Line
|
Type | Value | Description | References |
|---|---|---|---|---|
| L1210 | ED50 |
90 μM
Compound: 10
|
Compound tested for inhibition of the replication of L1210 cells in cell culture
Compound tested for inhibition of the replication of L1210 cells in cell culture
|
[PMID: 6244411] |
Chemical Information
-
CAS No. 59090-48-1
-
Appearance Solid
-
분자량 283.24
-
화학식 C10H13N5O5
-
Color White to off-white
-
SMILES
O=C(C(CN=[N+]=[N-])=CN1[C@H]2C[C@H](O)[C@@H](CO)O2)NC1=O
-
Synonyms
5-AmdU; α-Azidothymidine
-
선적
Room temperature in continental US; may vary elsewhere.
-
보관
-20°C, sealed storage, away from moisture
* In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)
용액&용해도
In Vitro:
DMSO : 50 mg/mL (176.53 mM; ultrasonic and warming and heat to 60°C; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Protocol
-
EdU Incorporation Assay (Click Chemistry-Based DNA Synthesis Measurement)
The EdU incorporation assay measures DNA synthesis by adding the thymidine analog 5-ethynyl-2′-deoxyuridine to cells or tissues, where it is incorporated into newly synthesized DNA during S phase. Incorporated EdU is detected by copper-catalyzed azide-alkyne cycloaddition, in which a fluorescent azide covalently reacts with the ethynyl group on EdU, allowing S-phase cells to be detected by fluorescence microscopy, flow cytometry, or high-content imaging. EdU detection does not require DNA denaturation or anti-BrdU antibody access, which preserves sample structure and improves compatibility with immunostaining and multiparameter cytometry compared with BrdU-based detection. EdU can be cytotoxic in a cell-type- and exposure-dependent manner, so pulse duration, concentration, and continuous-labeling designs should be validated for each cell type.
순도&문서
-
Data Sheet (273 KB)
-
SDS (252 KB)
- English - EN (252 KB)
- Français - FR (252 KB)
- Deutsch - DE (252 KB)
- Norwegian - NO (252 KB)
- Español - ES (252 KB)
- Swedish - SV (252 KB)
- Italian - IT (252 KB)
- Korean - KR (252 KB)
- Portuguese - PT (252 KB)
-
Handling Instructions (2659 KB)
References
[1]. Wen Z, Peng J, Tuttle PR, et al. Electron-Mediated Aminyl and Iminyl Radicals from C5 Azido-Modified Pyrimidine Nucleosides Augment Radiation Damage to Cancer Cells. Org Lett. 2018;20(23):7400-7404. [Content Brief]
[2]. Adjei D, Reyes Y, Kumar A, et al. Pathways of the Dissociative Electron Attachment Observed in 5- and 6-Azidomethyluracil Nucleosides: Nitrogen (N2) Elimination vs Azide Anion (N3-) Elimination. J Phys Chem B. 2023;127(7):1563-1571. [Content Brief]
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| DMSO | 1 mM | 3.5306 mL | 17.6529 mL | 35.3057 mL | 88.2644 mL |
| 5 mM | 0.7061 mL | 3.5306 mL | 7.0611 mL | 17.6529 mL | |
| 10 mM | 0.3531 mL | 1.7653 mL | 3.5306 mL | 8.8264 mL | |
| 15 mM | 0.2354 mL | 1.1769 mL | 2.3537 mL | 5.8843 mL | |
| 20 mM | 0.1765 mL | 0.8826 mL | 1.7653 mL | 4.4132 mL | |
| 25 mM | 0.1412 mL | 0.7061 mL | 1.4122 mL | 3.5306 mL | |
| 30 mM | 0.1177 mL | 0.5884 mL | 1.1769 mL | 2.9421 mL | |
| 40 mM | 0.0883 mL | 0.4413 mL | 0.8826 mL | 2.2066 mL | |
| 50 mM | 0.0706 mL | 0.3531 mL | 0.7061 mL | 1.7653 mL | |
| 60 mM | 0.0588 mL | 0.2942 mL | 0.5884 mL | 1.4711 mL | |
| 80 mM | 0.0441 mL | 0.2207 mL | 0.4413 mL | 1.1033 mL | |
| 100 mM | 0.0353 mL | 0.1765 mL | 0.3531 mL | 0.8826 mL |
5-Azidomethyl-2'-deoxyuridine Related Classifications
HY-154253 Related Classifications
Powered by Bioz