α-Synuclein (61-75) (TFA)

Name: α-Synuclein (61-75) (TFA)
Brand: MedChemExpress
SKU: HY-P3140A
Rating: 4.5 (1 reviews)

Cat. No.: HY-P3140A Purity: 99.62%: FAQs
Data Sheet SDS COA Handling Instructions

Molarity Calculator

α-Synuclein (61-75) TFA is the 61-75 fragment of α-Synuclein. α-Synuclein is an abundant neuronal protein that is highly enriched in presynaptic nerve terminals. α-Synuclein is a potential biomarker for Parkinson's disease (PD).

For research use only. We do not sell to patients.

Custom Peptide Synthesis

α-Synuclein (61-75) (TFA) Chemical Structure

Size	Price	Stock	Quantity
1 mg	USD 80	In-stock	Estimated Time of Arrival: December 31
5 mg	USD 240	In-stock	Estimated Time of Arrival: December 31
10 mg	USD 380	In-stock	Estimated Time of Arrival: December 31
25 mg	USD 760	In-stock	Estimated Time of Arrival: December 31
50 mg		Get quote
100 mg		Get quote

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Customer Review

Based on 1 publication(s) in Google Scholar

Other Forms of α-Synuclein (61-75) (TFA):

α-Synuclein (61-75) Get quote

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Biological Activity
Purity & Documentation
References
Customer Review

Description

In Vitro

Lewy bodies containing α-synuclein are a neuropathological hallmark of PD, and missense mutations in α-Synuclein (A30P, E46K, H50Q, G51D, A53E, A53T), as well as α-Synuclein gene duplications and triplications, appear to cause PD. Moreover, polymorphisms in regulatory elements of the α-Synuclein gene predispose individuals to PD and are linked to an early onset of the disease. The non-Aβ-amyloid component (NAC) region of α-synuclein is relatively hydrophobic and aggregation-prone in human α-Synuclein but not in mouse α-Synuclein nor in the corresponding homologous region of human β-synuclein. Yet, β-synuclein is more homologous to α-Synuclein in the N-terminal sequences (74%) than γ-synuclein (67%)^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Molecular Weight

1544.58

Formula

C₆₂H₁₀₄F₃N₁₇O₂₅

Appearance

Solid

Color

White to off-white

Sequence Shortening

EQVTNVGGAVVTGVT

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

Sealed storage, away from moisture

Powder	-80°C	2 years
	-20°C	1 year

*In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)

Solvent & Solubility

In Vitro:

DMSO : 100 mg/mL (64.74 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	0.6474 mL	3.2371 mL	6.4743 mL
5 mM	0.1295 mL	0.6474 mL	1.2949 mL
10 mM	0.0647 mL	0.3237 mL	0.6474 mL

View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

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Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: 2.5 mg/mL (1.62 mM); Suspended solution; Need ultrasonic

This protocol yields a suspended solution of 2.5 mg/mL. Suspended solution can be used for oral and intraperitoneal injection.
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Protocol 2

Add each solvent one by one: 10% DMSO 90% Corn Oil
Solubility: ≥ 2.5 mg/mL (1.62 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL Corn oil, and mix evenly.

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Please enter your animal formula composition:

DMSO +

Tween-80 +

Saline

Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.

The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).

Calculation results:

Working solution concentration: mg/mL

Method for preparing stock solution: mg drug dissolved in μL DMSO (Stock solution concentration: mg/mL).

*In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)

The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).

Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.

If the continuous dosing period exceeds half a month, please choose this protocol carefully.

Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.

Purity & Documentation

Purity: 99.62%

Select Batch:

Data Sheet (271 KB) SDS (252 KB)

COA (243 KB) LCMS (98 KB) Elemental Analysis Report (102 KB)

Handling Instructions (2659 KB)

References

[1]. Jacqueline Burré, et al. Cell Biology and Pathophysiology of α-Synuclein. Cold Spring Harb Perspect Med. 2018 Mar 1;8(3):a024091. [Content Brief]

[2]. Nelson Ferreira, et al. Trans-synaptic spreading of alpha-synuclein pathology through sensory afferents leads to sensory nerve degeneration and neuropathic pain. Acta Neuropathol Commun. 2021 Feb 25;9(1):31. [Content Brief]

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg

DMSO	1 mM	0.6474 mL	3.2371 mL	6.4743 mL	16.1856 mL
	5 mM	0.1295 mL	0.6474 mL	1.2949 mL	3.2371 mL
	10 mM	0.0647 mL	0.3237 mL	0.6474 mL	1.6186 mL
	15 mM	0.0432 mL	0.2158 mL	0.4316 mL	1.0790 mL
	20 mM	0.0324 mL	0.1619 mL	0.3237 mL	0.8093 mL
	25 mM	0.0259 mL	0.1295 mL	0.2590 mL	0.6474 mL
	30 mM	0.0216 mL	0.1079 mL	0.2158 mL	0.5395 mL
	40 mM	0.0162 mL	0.0809 mL	0.1619 mL	0.4046 mL
	50 mM	0.0129 mL	0.0647 mL	0.1295 mL	0.3237 mL
	60 mM	0.0108 mL	0.0540 mL	0.1079 mL	0.2698 mL