2-Hydroxy-2-methylbutanoic acid
Based on 1 Customer Validation
2-Hydroxy-2-methylbutanoic acid is a branched-chain fatty acid. 2-Hydroxy-2-methylbutanoic acid accumulates at high levels in the culture supernatants of colorectal cancer-associated bacteria. 2-Hydroxy-2-methylbutanoic acid is also present in the urine of patients with 2-hydroxyglutaric aciduria and maple syrup urine disease.
For research use only. We do not sell to patients.
- Purity: 98.0%
- CAS No.: 3739-30-8
- Formula: C5H10O3
- Molecular Weight:118.13
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Storage:Powder -20°C, 3 years , 4°C, 2 years ; In solvent -80°C, 6 months , -20°C, 1 month
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Tel: 609-228-6898 E-mail: [email protected] [email protected]
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Biological Activity
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Chemical Information
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Solvent & Solubility
- Purity & Documentation
- References
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Biological Activity
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Human Endogenous Metabolite |
2-Hydroxy-2-methylbutanoic acid accumulates at high levels in the culture supernatants of colorectal cancer-associated bacteria, and is also present in the urine with 2-hydroxyglutaric aciduria and maple syrup urine disease[1].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
Chemical Information
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CAS No. 3739-30-8
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Appearance Solid
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Molecular Weight 118.13
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Formula C5H10O3
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Color White to off-white
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SMILES
CCC(C)(O)C(O)=O
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Structure Classification
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Initial Source
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month
Solvent & Solubility
DMSO : 100 mg/mL (846.53 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Select the appropriate dissolution method based on your experimental animal and administration route.
- For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
- To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
- The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.
Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 2.5 mg/mL (21.16 mM); Clear solution
This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: ≥ 2.5 mg/mL (21.16 mM); Clear solution
This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
Please enter the basic information of animal experiments:
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Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
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%DMSO +
Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
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%+
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+%Tween-80 + +
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%Saline +
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Working solution concentration: 0.22 mg/mL
Method for preparing stock solution: mg drug dissolved in μL DMSO. Stock solution concentration: mg/mL.
1. Take μL DMSO stock solution;
2. Add μL .
μL , mix evenly;
3. Then add μL Tween 80, mix evenly;
4. Then add μL
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
Purity & Documentation
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Data Sheet (272 KB)
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SDS (394 KB)
- English - EN (394 KB)
- Français - FR (394 KB)
- Deutsch - DE (394 KB)
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- Español - ES (394 KB)
- Swedish - SV (394 KB)
- Italian - IT (394 KB)
- Korean - KR (394 KB)
- Portuguese - PT (394 KB)
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Handling Instructions (2659 KB)
References
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| DMSO | 1 mM | 8.4653 mL | 42.3263 mL | 84.6525 mL | 211.6313 mL |
| 5 mM | 1.6931 mL | 8.4653 mL | 16.9305 mL | 42.3263 mL | |
| 10 mM | 0.8465 mL | 4.2326 mL | 8.4653 mL | 21.1631 mL | |
| 15 mM | 0.5644 mL | 2.8218 mL | 5.6435 mL | 14.1088 mL | |
| 20 mM | 0.4233 mL | 2.1163 mL | 4.2326 mL | 10.5816 mL | |
| 25 mM | 0.3386 mL | 1.6931 mL | 3.3861 mL | 8.4653 mL | |
| 30 mM | 0.2822 mL | 1.4109 mL | 2.8218 mL | 7.0544 mL | |
| 40 mM | 0.2116 mL | 1.0582 mL | 2.1163 mL | 5.2908 mL | |
| 50 mM | 0.1693 mL | 0.8465 mL | 1.6931 mL | 4.2326 mL | |
| 60 mM | 0.1411 mL | 0.7054 mL | 1.4109 mL | 3.5272 mL | |
| 80 mM | 0.1058 mL | 0.5291 mL | 1.0582 mL | 2.6454 mL | |
| 100 mM | 0.0847 mL | 0.4233 mL | 0.8465 mL | 2.1163 mL |
2-Hydroxy-2-methylbutanoic acid Related Classifications
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