2'-Deoxyadenosine-5'-triphosphate
2'-Deoxyadenosine-5'-triphosphate (dATP) is a nucleotide used in cells for DNA synthesis (or replication), as a substrate of DNA polymerase.
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- CAS No.: 1927-31-7
- Formula: C10H16N5O12P3
- Molecular Weight:491.18
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Storage:
Please store the product under the recommended conditions in the Certificate of Analysis.
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Biological Activity
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Chemical Information
- Purity & Documentation
- References
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Help & FAQs
Biological Activity
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DNA Polymerase |
Human Endogenous Metabolite |
2'-Deoxyadenosine-5'-triphosphate (dATP) (0.01-0.5 mM; 600 sec) induces dose-dependent mitochondrial permeability transition in isolated mouse liver mitochondria, with a half-maximal response at 0.03 mM, and this induction is sensitive to cyclosporin A but not z-VAD[2].
dATP is required for apoptosome assembly and subsequent procaspase-9 activation in cell-free extracts, a key step in the intrinsic apoptotic pathway[3].
dATP directly induces specific cytochrome c release from isolated mitochondria, initiating a key step in the intrinsic apoptotic pathway[3].
2'-Deoxyadenosine-5'-triphosphate (dATP) (400 μM; 2 h) increases Escherichia coli K-12 membrane-damaged attached cells by over 2-fold and total attached cells by ~1.6-fold, and (400 μM; 2 h in planktonic cultures or 16 h in biofilms) increases eDNA release by 48% in planktonic cells and 200% in biofilm matrices[4].
2'-Deoxyadenosine-5'-triphosphate (dATP) (400 μM; 60 min) increases Acinetobacter baumannii adherence to human bronchial epithelial NCI-H292 cells by 100-fold, from 1.6 × 104 to 8.79 × 105 CFU/cm2, and promotes bacterial aggregate formation[4].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
| NCT Number | Sponsor | Condition | Start Date |
Phase
|
|---|---|---|---|---|
| NCT01329991 | Plexxikon| | 2011-05 | PHASE1 |
Chemical Information
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CAS No. 1927-31-7
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Molecular Weight 491.18
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Formula C10H16N5O12P3
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SMILES
O[C@H]([C@H](O1)CO[P](O[P](O[P](O)(O)=O)(O)=O)(O)=O)C[C@@H]1N2C3=NC=NC(N)=C3N=C2
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Synonyms
dATP
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Structure Classification
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
Please store the product under the recommended conditions in the Certificate of Analysis.
Purity & Documentation
References
[1]. P J Romaniuk, A study of the mechanism of T4 DNA polymerase with diastereomeric phosphorothioate analogues of deoxyadenosine triphosphate. J Biol Chem. 1982 Jul 10;257(13):7684-8. [Content Brief]
[2]. Yang JC, Cortopassi GA. dATP causes specific release of cytochrome C from mitochondria. Biochem Biophys Res Commun. 1998 Sep 18;250(2):454-7. [Content Brief]
[3]. Katona K, et al. Deoxy-adenosine-monophosphate (dAMP) di-n-butylester induces apoptosis by increasing the dATP level in HL-60 cells. Cancer Lett. 2006 Apr 28;235(2):281-90. [Content Brief]
[4]. Xi C, Wu J. dATP/ATP, a multifunctional nucleotide, stimulates bacterial cell lysis, extracellular DNA release and biofilm development. PLoS One. 2010 Oct 14;5(10):e13355. [Content Brief]
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
2'-Deoxyadenosine-5'-triphosphate Related Classifications
HY-136648 Related Classifications
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