2-Keto-D-Glucose  (Synonyms: D-Glucosone; D-Arabino-hexos-2-ulose)

2-Keto-D-Glucose (D-Glucosone) is a key intermediate in a secondary metabolic pathway leading to the antibiotic Cortalcerone. 2-Keto-D-Glucose is also an intermediate in the conversion of D-glucose into D-fructose. 2-Keto-D-Glucose is found in various natural sources, including fungi, algae, and shellfish^[1][2].

Pyrroloquinoline quinone-dependent 2-keto-D-glucose (2KG) dehydrogenase (2KGDH) has high specificity for the oxidation of 2-Keto-D-Glucose to 2-keto-D-gluconic acid (2KGA). P. aureofaciens (Pa2KGDH) specifically preferred 2KG as a substrate and oxidized the C-1 position of 2KG, indicating that the enzyme is a 2KGDH^[3].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

178.14

C₆H₁₀O₆

1854-25-7

Solid

Off-white to light yellow

OC[C@H]([C@H]([C@@H](C(C=O)=O)O)O)O

Room temperature in continental US; may vary elsewhere.

-20°C, protect from light, stored under nitrogen

*In solvent : -80°C, 6 months; -20°C, 1 month (protect from light, stored under nitrogen)

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light, stored under nitrogen). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

• [1]. Sun, Lianhong, et al. Engineering galactose oxidase to increase expression level in E. coli, enhance thermostability, and introduce novel activities. Dissertation (Ph.D.), California Institute of Technology.

  [2]. zawa K, et al. A novel pyrroloquinoline quinone-dependent 2-keto-D-glucose dehydrogenase from Pseudomonas aureofaciens. J Bacteriol. 2015 Apr;197(8):1322-9.  [Content Brief]

  [3]. Te-ning E.Liu, et al. Convenient, laboratory procedure for producing solid d-arabino-hexos-2-ulose (d-glucosone). Carbohydrate Research. Volume 113, Issue 1, 16 February 1983, Pages 151-157.