2-Pyridinecarbohydrazide
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2-Pyridinecarbohydrazide is a versatile heterocyclic building block widely used in medicinal chemistry and coordination chemistry, and it finds extensive applications in various synthetic fields. 2-Pyridinecarbohydrazide reacts with the reducing end of carbohydrates to enhance mass spectrometry ionization efficiency, enabling isomer-specific identification of monosaccharides, disaccharides, ketoses/aldoses, and quantitative mass spectrometry imaging of biological tissues.
For research use only. We do not sell to patients.
- Purity: 99.8%
- CAS No.: 1452-63-7
- Formula: C6H7N3O
- Molecular Weight:137.14
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Storage:Powder -20°C, 3 years , 4°C, 2 years ; In solvent -80°C, 6 months , -20°C, 1 month
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Tel: 609-228-6898 E-mail: [email protected] [email protected]
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Biological Activity
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Chemical Information
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Solvent & Solubility
- Purity & Documentation
- References
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Help & FAQs
Biological Activity
2-Pyridinecarbohydrazide (PYD) (1 mg/mL; 4 h) enhances the MALDI-MS signals of monosaccharide and disaccharide standards by more than 220-fold, distinguishes aldose/ketose monosaccharide isomers, and identifies the linkage type, anomeric configuration, and composition of disaccharide isomers via unique MS/MS fragment ions and ion intensity ratios[2].
2-Pyridinecarbohydrazide (4 h) enables on-tissue derivatization for spatial mapping of aldohexose and ketohexose isomers in Fragaria × ananassa (strawberry) tissue sectionss[2].
2-Pyridinecarbohydrazide (4 h) enables tissue derivatization for spatial localization of α,α-1,6-linked Glcα1-6Fru disaccharide in Vitis vinifera tissue sections[2].
2-Pyridinecarbohydrazide (1 mg/mL; 4 h) enables quantitative MALDI-MS/MS analysis of disaccharide isomers[2].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
Chemical Information
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CAS No. 1452-63-7
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Appearance Solid
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Molecular Weight 137.14
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Formula C6H7N3O
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Color White to off-white
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SMILES
O=C(C1=NC=CC=C1)NN
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month
Solvent & Solubility
DMSO : 100 mg/mL (729.18 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Select the appropriate dissolution method based on your experimental animal and administration route.
- For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
- To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
- The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.
Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 2.5 mg/mL (18.23 mM); Clear solution
This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: ≥ 2.5 mg/mL (18.23 mM); Clear solution
This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
Please enter the basic information of animal experiments:
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Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
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%DMSO +
Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
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%+
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+%Tween-80 + +
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%Saline +
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Working solution concentration: 0.22 mg/mL
Method for preparing stock solution: mg drug dissolved in μL DMSO. Stock solution concentration: mg/mL.
1. Take μL DMSO stock solution;
2. Add μL .
μL , mix evenly;
3. Then add μL Tween 80, mix evenly;
4. Then add μL
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
Purity & Documentation
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Data Sheet (273 KB)
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SDS (392 KB)
- English - EN (392 KB)
- Français - FR (392 KB)
- Deutsch - DE (392 KB)
- Norwegian - NO (392 KB)
- Español - ES (392 KB)
- Swedish - SV (392 KB)
- Italian - IT (392 KB)
- Korean - KR (392 KB)
- Portuguese - PT (392 KB)
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Handling Instructions (2659 KB)
References
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
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| DMSO | 1 mM | 7.2918 mL | 36.4591 mL | 72.9182 mL | 182.2955 mL |
| 5 mM | 1.4584 mL | 7.2918 mL | 14.5836 mL | 36.4591 mL | |
| 10 mM | 0.7292 mL | 3.6459 mL | 7.2918 mL | 18.2295 mL | |
| 15 mM | 0.4861 mL | 2.4306 mL | 4.8612 mL | 12.1530 mL | |
| 20 mM | 0.3646 mL | 1.8230 mL | 3.6459 mL | 9.1148 mL | |
| 25 mM | 0.2917 mL | 1.4584 mL | 2.9167 mL | 7.2918 mL | |
| 30 mM | 0.2431 mL | 1.2153 mL | 2.4306 mL | 6.0765 mL | |
| 40 mM | 0.1823 mL | 0.9115 mL | 1.8230 mL | 4.5574 mL | |
| 50 mM | 0.1458 mL | 0.7292 mL | 1.4584 mL | 3.6459 mL | |
| 60 mM | 0.1215 mL | 0.6077 mL | 1.2153 mL | 3.0383 mL | |
| 80 mM | 0.0911 mL | 0.4557 mL | 0.9115 mL | 2.2787 mL | |
| 100 mM | 0.0729 mL | 0.3646 mL | 0.7292 mL | 1.8230 mL |
2-Pyridinecarbohydrazide Related Classifications
HY-W010341 Related Classifications
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