22,23-Dihydroavermectin B1a aglycon
Based on 1 Customer Validation
22,23-Dihydroavermectin B1a aglycon is an acid degradation product produced by hydrolysis of the disaccharide unit of ivermectin. It can inhibit nematode larval development, but does not cause paralytic activity.
For research use only. We do not sell to patients.
- Purity: 99.54%
- CAS No.: 73162-95-5
- Formula: C34H50O8
- Molecular Weight:586.76
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Storage:Powder -20°C, 3 years , 4°C, 2 years ; In solvent -80°C, 6 months , -20°C, 1 month
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
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Biological Activity
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Chemical Information
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Solvent & Solubility
- Purity & Documentation
- References
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Help & FAQs
All Parasite Isoforms
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Biological Activity
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Cell Line
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Type | Value | Description | References |
|---|---|---|---|---|
| HL-60 | GI50 |
35.2 μM
Compound: 2
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Cytotoxicity against human HL-60 cells assessed as cell growth inhibition measured after 24 hrs by resazurin dye based microplate reader analysis
Cytotoxicity against human HL-60 cells assessed as cell growth inhibition measured after 24 hrs by resazurin dye based microplate reader analysis
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[PMID: 37988797] |
| Macrophage | IC50 |
>90 μM
Compound: 3
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Cytotoxicity against mouse peritoneal macrophages assessed as LDH release after 48 hrs
Cytotoxicity against mouse peritoneal macrophages assessed as LDH release after 48 hrs
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[PMID: 19114308] |
Chemical Information
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CAS No. 73162-95-5
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Appearance Solid
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Molecular Weight 586.76
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Formula C34H50O8
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Color White to off-white
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SMILES
C[C@H]([C@@]1([H])O[C@]2(CC[C@@H]1C)C[C@](OC([C@@](C=C3C)([H])[C@]4([C@]([C@@H]3O)([H])OC/C4=C\C=C\[C@@H]5C)O)=O)([H])C[C@](C/C=C([C@H]5O)\C)([H])O2)CC
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Synonyms
Ivermectin Impurity G
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month
Solvent & Solubility
DMSO : 50 mg/mL (85.21 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Select the appropriate dissolution method based on your experimental animal and administration route.
- For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
- To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
- The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.
Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 1.25 mg/mL (2.13 mM); Clear solution
This protocol yields a clear solution of ≥ 1.25 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (12.5 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: ≥ 1.25 mg/mL (2.13 mM); Clear solution
This protocol yields a clear solution of ≥ 1.25 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (12.5 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
Please enter the basic information of animal experiments:
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Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
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%DMSO +
Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
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%+
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+%Tween-80 + +
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%Saline +
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Working solution concentration: 0.22 mg/mL
Method for preparing stock solution: mg drug dissolved in μL DMSO. Stock solution concentration: mg/mL.
1. Take μL DMSO stock solution;
2. Add μL .
μL , mix evenly;
3. Then add μL Tween 80, mix evenly;
4. Then add μL
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
Purity & Documentation
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Data Sheet (279 KB)
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SDS (557 KB)
- English - EN (557 KB)
- Français - FR (557 KB)
- Deutsch - DE (557 KB)
- Norwegian - NO (557 KB)
- Español - ES (557 KB)
- Swedish - SV (557 KB)
- Italian - IT (557 KB)
- Korean - KR (557 KB)
- Portuguese - PT (557 KB)
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Handling Instructions (2659 KB)
References
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| DMSO | 1 mM | 1.7043 mL | 8.5214 mL | 17.0427 mL | 42.6069 mL |
| 5 mM | 0.3409 mL | 1.7043 mL | 3.4085 mL | 8.5214 mL | |
| 10 mM | 0.1704 mL | 0.8521 mL | 1.7043 mL | 4.2607 mL | |
| 15 mM | 0.1136 mL | 0.5681 mL | 1.1362 mL | 2.8405 mL | |
| 20 mM | 0.0852 mL | 0.4261 mL | 0.8521 mL | 2.1303 mL | |
| 25 mM | 0.0682 mL | 0.3409 mL | 0.6817 mL | 1.7043 mL | |
| 30 mM | 0.0568 mL | 0.2840 mL | 0.5681 mL | 1.4202 mL | |
| 40 mM | 0.0426 mL | 0.2130 mL | 0.4261 mL | 1.0652 mL | |
| 50 mM | 0.0341 mL | 0.1704 mL | 0.3409 mL | 0.8521 mL | |
| 60 mM | 0.0284 mL | 0.1420 mL | 0.2840 mL | 0.7101 mL | |
| 80 mM | 0.0213 mL | 0.1065 mL | 0.2130 mL | 0.5326 mL |
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