3-Chlorodiphenylamine 

3-Chlorodiphenylamine is a high affinity Ca²⁺ sensitizer of cardiac muscle. 3-Chlorodiphenylamine is based on diphenylamine and binds to the isolated N-domain of cardiac troponin C (cTnC) (K_d=6 µM). 3-Chlorodiphenylamine is an excellent starting scaffold for the development of more potent Ca²⁺-sensitizing compounds due to its small size, and can be used for systolic heart failure research^[1].

Kd: 6 µM (N-domain of cardiac troponin C (cTnC))
Kd: 10 µM (cNTnC–cSp chimera)^[1]

3-Chlorodiphenylamine is able to bind highly (K_d=10 µM) to a chimeric protein consisting of the regulatory N-domain of cTnC (cNTnC) and the switch region of cTnI (cNTnC–cSp chimera)^[1].
3-Chlorodiphenylamine (100 µM) results in a 1.5-fold increase in the Ca²⁺ sensitivity of force development without altering the maximal or resting forces in skinned ventricular trabeculae^[1].
3-Chlorodiphenylamine (25-100 µM) increases Ca²⁺ sensitivity of the N-domain of intact cTnC after reconstitution into the cTn complex (cTnC complexed with cTnI and cTnT) in a concentration-dependent manner. It exhibits pCa₅₀s with 6.39±0.01, 6.65±0.01, and 6.73±0.02 in the presence of 25, 50, and 100 µM 3-Chlorodiphenylamine, respectively^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

203.67

C₁₂H₁₀ClN

101-17-7

Liquid

Light yellow to yellow

ClC1=CC(NC2=CC=CC=C2)=CC=C1

Room temperature in continental US; may vary elsewhere.

Please store the product under the recommended conditions in the Certificate of Analysis.

• [1]. Svetlana B Tikunova, et al. 3-Chlorodiphenylamine activates cardiac troponin by a mechanism distinct from bepridil or TFP. J Gen Physiol. 2019 Jan 7;151(1):9-17.  [Content Brief]