3-Chlorophenyl boronic acid
Based on 1 Customer Validation
3-Chlorophenylboronic acid is employed as a reactant involved in 1,4-conjugate addition reactions with ethenesulfonamides to form arylethanesulfonamides, Suzuki-Miyaura reactions with dibromotrifluoromethylbenzene, cross-coupling reactions with diazoesters3 or potassium cyanate. 3-Chlorophenylboronic acid is involved in the synthesis of biarylketones and phthalides and synthesis of inhibitors including PDE4 inhibitors among others. 3-Chlorophenyl boronic acid can affect cell migration. 3-Chlorophenyl boronic acid can be used to study wound healing .
For research use only. We do not sell to patients.
- Purity: 99.88%
- CAS No.: 63503-60-6
- Formula: C6H6BClO2
- Molecular Weight:156.38
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Storage:Powder -20°C, 3 years , 4°C, 2 years ; In solvent -80°C, 6 months , -20°C, 1 month
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
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Biological Activity
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Chemical Information
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Solvent & Solubility
- Purity & Documentation
- References
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Help & FAQs
Biological Activity
When the concentration of 3-Chlorophenyl boronic acid is 3.90-250 µg/mL, the cell survival rate can reach 64-109%, but when the concentration is 500 µg/mL, the survival rate drops to 17%[1].
The cell migration effect of 3-Chlorophenyl boronic acid intensifies over time[1].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
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Cell Line:L929
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Concentration:500, 250, 125, 62.5, 31.25, 15.62, 7.81, 3.90 µg/mL
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Incubation Time:22 h
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Result:Applied at a concentration of 500 µg/mL, the percentage viability rate was quite low (17.5%), but a significant increase in viability was observed at the concentration applied at 250 µg/mL, and this rate was approximately 64%.
The concentration with a significant increase in viability was determined as 62.5 µg/mL (93%).
The concentration that showed the highest cell viability at lower concentrations was 15.62 µg/mL (109%).
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Cell Line:L929
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Concentration:15.62 µg/mL
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Incubation Time:0, 12, 24 h
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Result:There was a cell migration of 7.98 inches at the 0th hour, 5.18 inches at the 12th hour, and 4.76 inches at the 24th hour.
A wound closure of 35% was detected at the 12th hour and 40% at the 24th hour.
Chemical Information
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CAS No. 63503-60-6
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Appearance Solid
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Molecular Weight 156.38
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Formula C6H6BClO2
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Color White to off-white
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SMILES
ClC1=CC=CC(B(O)O)=C1
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month
Solvent & Solubility
DMSO : ≥ 175 mg/mL (1119.07 mM; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
* "≥" means soluble, but saturation unknown.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Select the appropriate dissolution method based on your experimental animal and administration route.
- For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
- To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
- The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.
Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: ≥ 5 mg/mL (31.97 mM); Clear solution
This protocol yields a clear solution of ≥ 5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (50.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
Please enter the basic information of animal experiments:
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Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
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%DMSO +
Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
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%+
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+%Tween-80 + +
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%Saline +
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Working solution concentration: 0.22 mg/mL
Method for preparing stock solution: mg drug dissolved in μL DMSO. Stock solution concentration: mg/mL.
1. Take μL DMSO stock solution;
2. Add μL .
μL , mix evenly;
3. Then add μL Tween 80, mix evenly;
4. Then add μL
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
Purity & Documentation
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Data Sheet (275 KB)
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SDS (394 KB)
- English - EN (394 KB)
- Français - FR (394 KB)
- Deutsch - DE (394 KB)
- Norwegian - NO (394 KB)
- Español - ES (394 KB)
- Swedish - SV (394 KB)
- Italian - IT (394 KB)
- Korean - KR (394 KB)
- Portuguese - PT (394 KB)
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Handling Instructions (2659 KB)
References
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
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| DMSO | 1 mM | 6.3947 mL | 31.9734 mL | 63.9468 mL | 159.8670 mL |
| 5 mM | 1.2789 mL | 6.3947 mL | 12.7894 mL | 31.9734 mL | |
| 10 mM | 0.6395 mL | 3.1973 mL | 6.3947 mL | 15.9867 mL | |
| 15 mM | 0.4263 mL | 2.1316 mL | 4.2631 mL | 10.6578 mL | |
| 20 mM | 0.3197 mL | 1.5987 mL | 3.1973 mL | 7.9933 mL | |
| 25 mM | 0.2558 mL | 1.2789 mL | 2.5579 mL | 6.3947 mL | |
| 30 mM | 0.2132 mL | 1.0658 mL | 2.1316 mL | 5.3289 mL | |
| 40 mM | 0.1599 mL | 0.7993 mL | 1.5987 mL | 3.9967 mL | |
| 50 mM | 0.1279 mL | 0.6395 mL | 1.2789 mL | 3.1973 mL | |
| 60 mM | 0.1066 mL | 0.5329 mL | 1.0658 mL | 2.6644 mL | |
| 80 mM | 0.0799 mL | 0.3997 mL | 0.7993 mL | 1.9983 mL | |
| 100 mM | 0.0639 mL | 0.3197 mL | 0.6395 mL | 1.5987 mL |
3-Chlorophenyl boronic acid Related Classifications
HY-W002463 Related Classifications
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