3-Demethylcolchicine
Based on 1 Customer Validation
3-Demethylcolchicine, a colchicine metabolite and anti-inflammatory agent, exhibits potent tubulin-binding activity. 3-Demethylcolchicine inhibits carrageenan-induced rat paw edema. 3-Demethylcolchicine bears a hydroxyl group on its carbocyclic ring; this group participates in free radical scavenging processes, allowing it to act as a free radical scavenger. 3-Demethylcolchicine is applicable to studies related to carrageenan-induced paw edema.
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- Purity: 98.73%
- CAS No.: 7336-33-6
- 화학식: C21H23NO6
- 분자량:385.41
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보관:
4°C, protect from light
* In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)
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Biological Activity
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Chemical Information
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- 순도&문서
- References
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Help & FAQs
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Biological Activity
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Cell Line
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Type | Value | Description | References |
|---|---|---|---|---|
| MCF7 | EC50 |
0.18 μM
Compound: 2
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Cytotoxicity against human MCF7 after 3 days by SRB assay
Cytotoxicity against human MCF7 after 3 days by SRB assay
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[PMID: 15730238] |
| NCI-H460 | EC50 |
0.51 μM
Compound: 2
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Cytotoxicity against human H460 after 3 days by SRB assay
Cytotoxicity against human H460 after 3 days by SRB assay
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[PMID: 15730238] |
| SF-268 | EC50 |
0.28 μM
Compound: 2
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Cytotoxicity against human SF-268 after 3 days by SRB assay
Cytotoxicity against human SF-268 after 3 days by SRB assay
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[PMID: 15730238] |
3-Demethylcolchicine (25 μM) exhibits strong in vitro binding to rat brain tubulin, reducing tritiated colchicine binding by 68% at a concentration of 25 μM[1].
3-Demethylcolchicine (1-100 μM; 15 min pre-incubation, 1 h H2O2 exposure) does not prevent H2O2-induced GSH oxidation in HL-60 cells, with only minimal weak protection at the lowest tested concentrations[2].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
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Animal Model:Donryu (male, 6 weeks old, 140-160 g, carrageenin-induced footpad edema)[1]
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Dosage:100 μg per foot
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Administration:subplantar (local footpad injection); single dose
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Result:Inhibited footpad edema by 39% at 3 hours post-injection.
Inhibited footpad edema by 47% at 5 hours post-injection.
Chemical Information
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CAS No. 7336-33-6
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Appearance Solid
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분자량 385.41
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화학식 C21H23NO6
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Color White to yellow
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SMILES
CC(N[C@H](C1=C2)CCC3=CC(O)=C(OC)C(OC)=C3C1=CC=C(OC)C2=O)=O
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Structure Classification
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Initial Source
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선적
Room temperature in continental US; may vary elsewhere.
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보관
4°C, protect from light
* In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)
용액&용해도
DMSO : 100 mg/mL (259.46 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Select the appropriate dissolution method based on your experimental animal and administration route.
- For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
- To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
- The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.
Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 2.5 mg/mL (6.49 mM); Clear solution
This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: ≥ 2.5 mg/mL (6.49 mM); Clear solution
This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
Please enter the basic information of animal experiments:
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Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
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%DMSO +
Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
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%+
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+%Tween-80 + +
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%Saline +
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Working solution concentration: 0.22 mg/mL
Method for preparing stock solution: mg drug dissolved in μL DMSO. Stock solution concentration: mg/mL. * In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)
1. Take μL DMSO stock solution;
2. Add μL .
μL , mix evenly;
3. Then add μL Tween 80, mix evenly;
4. Then add μL
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
순도&문서
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Data Sheet (283 KB)
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SDS (393 KB)
- English - EN (393 KB)
- Français - FR (393 KB)
- Deutsch - DE (393 KB)
- Norwegian - NO (393 KB)
- Español - ES (393 KB)
- Swedish - SV (393 KB)
- Italian - IT (393 KB)
- Korean - KR (393 KB)
- Portuguese - PT (393 KB)
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Handling Instructions (2659 KB)
References
[1]. Sugio K, et al. Separation of tubulin-binding and anti-inflammatory activity in colchicine analogs and congeners. Life Sci. 1987;40(1):35-39. [Content Brief]
[2]. Modriansky M, et al. Anti-/pro-oxidant effects of phenolic compounds in cells: are colchicine metabolites chain-breaking antioxidants?. Toxicology. 2002;177(1):105-117. [Content Brief]
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| DMSO | 1 mM | 2.5946 mL | 12.9732 mL | 25.9464 mL | 64.8660 mL |
| 5 mM | 0.5189 mL | 2.5946 mL | 5.1893 mL | 12.9732 mL | |
| 10 mM | 0.2595 mL | 1.2973 mL | 2.5946 mL | 6.4866 mL | |
| 15 mM | 0.1730 mL | 0.8649 mL | 1.7298 mL | 4.3244 mL | |
| 20 mM | 0.1297 mL | 0.6487 mL | 1.2973 mL | 3.2433 mL | |
| 25 mM | 0.1038 mL | 0.5189 mL | 1.0379 mL | 2.5946 mL | |
| 30 mM | 0.0865 mL | 0.4324 mL | 0.8649 mL | 2.1622 mL | |
| 40 mM | 0.0649 mL | 0.3243 mL | 0.6487 mL | 1.6216 mL | |
| 50 mM | 0.0519 mL | 0.2595 mL | 0.5189 mL | 1.2973 mL | |
| 60 mM | 0.0432 mL | 0.2162 mL | 0.4324 mL | 1.0811 mL | |
| 80 mM | 0.0324 mL | 0.1622 mL | 0.3243 mL | 0.8108 mL | |
| 100 mM | 0.0259 mL | 0.1297 mL | 0.2595 mL | 0.6487 mL |
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