4-Hydroxymethylpyrazole
Based on 1 Customer Validation
4-Hydroxymethylpyrazole is the major phase I oxidative metabolite of 4-Methylpyrazole (HY-B0876) in rats and can be further oxidized to form 4-Carboxypyrazole (HY-W002065). 4-Hydroxymethylpyrazole acts as a substrate and competitive inhibitor of alcohol dehydrogenase (ADH). 4-Hydroxymethylpyrazole can be used in metabolism-related research.
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- Pureté : 99.74%
- CAS No.: 25222-43-9
- Formule: C4H6N2O
- Masse moléculaire:98.11
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Stockage:Powder -20°C, 3 years , 4°C, 2 years ; In solvent -80°C, 6 months , -20°C, 1 month
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Activité biologique
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Chemical Information
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Solvant et solubilité
- Pureté et documentation
- Références
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Help & FAQs
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HY-B0876
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HY-I0314
1H-Pyrazole-4-boronic acid pinacol esterDrug Intermediate -
HY-W002065
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Ethyl 5-methyl-1H-pyrazole-3-carboxylateBiochemical Reagent -
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Ethyl 3-trifluoromethylpyrazole-4-carboxylateBiochemical Reagent
Activité biologique
Description
In Vitro
4-Hydroxymethylpyrazole is a competitive inhibitor of human liver ADH with a Ki of 6.6 μM, and it also serves as a substrate for this enzyme with a Km of approximately 200 μM[1].
4-Hydroxymethylpyrazole is the major urinary metabolite of 4-Methylpyrazole (HY-B0876), formed by methyl hydroxylation, and can be further oxidized to 4-Carboxypyrazole (HY-W002065)[2].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only. .
Chemical Information
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CAS No. 25222-43-9
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Appearance Solid
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Masse moléculaire 98.11
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Formule C4H6N2O
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Color White to off-white
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SMILES
OCC1=CNN=C1
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Structure Classification
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Initial Source
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Livraison
Room temperature in continental US; may vary elsewhere.
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Stockage
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month
Solvant et solubilité
In Vitro:
DMSO : 100 mg/mL (1019.26 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
H2O : 100 mg/mL (1019.26 mM; Need ultrasonic)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
In Vivo:
Select the appropriate dissolution method based on your experimental animal and administration route.
- For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
- To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
- The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.
Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 2.5 mg/mL (25.48 mM); Clear solution
This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: ≥ 2.5 mg/mL (25.48 mM); Clear solution
This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:
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Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Working solution concentration: 0.22 mg/mL
This product has good water solubility, please refer to the measured solubility data in water/PBS/Saline for details.
Pureté et documentation
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Fiche technique (271 KB)
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SDS (394 KB)
- English - EN (394 KB)
- Français - FR (394 KB)
- Deutsch - DE (394 KB)
- Norwegian - NO (394 KB)
- Español - ES (394 KB)
- Swedish - SV (394 KB)
- Italian - IT (394 KB)
- Korean - KR (394 KB)
- Portuguese - PT (394 KB)
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Instruction de manipulation (2659 KB)
Références
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| DMSO / H2O | 1 mM | 10.1926 mL | 50.9632 mL | 101.9264 mL | 254.8160 mL |
| 5 mM | 2.0385 mL | 10.1926 mL | 20.3853 mL | 50.9632 mL | |
| 10 mM | 1.0193 mL | 5.0963 mL | 10.1926 mL | 25.4816 mL | |
| 15 mM | 0.6795 mL | 3.3975 mL | 6.7951 mL | 16.9877 mL | |
| 20 mM | 0.5096 mL | 2.5482 mL | 5.0963 mL | 12.7408 mL | |
| 25 mM | 0.4077 mL | 2.0385 mL | 4.0771 mL | 10.1926 mL | |
| 30 mM | 0.3398 mL | 1.6988 mL | 3.3975 mL | 8.4939 mL | |
| 40 mM | 0.2548 mL | 1.2741 mL | 2.5482 mL | 6.3704 mL | |
| 50 mM | 0.2039 mL | 1.0193 mL | 2.0385 mL | 5.0963 mL | |
| 60 mM | 0.1699 mL | 0.8494 mL | 1.6988 mL | 4.2469 mL | |
| 80 mM | 0.1274 mL | 0.6370 mL | 1.2741 mL | 3.1852 mL | |
| 100 mM | 0.1019 mL | 0.5096 mL | 1.0193 mL | 2.5482 mL |
* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.
4-Hydroxymethylpyrazole Related Classifications
HY-33914 Related Classifications
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