5,7,3',4'-Tetramethoxyflavone
Based on 2 publication(s) in Google Scholar
5,7,3',4'-Tetramethoxyflavone, an orally active polymethoxyflavones (PMFs) that can be isolated from M. exotica, possesses various bioactivities, including anti-fungal, anti-malarial, anti-mycobacterial, and anti-inflammatory activities. 5,7,3',4'-Tetramethoxyflavone exhibits chondroprotective activity by targeting β-catenin signaling.
For research use only. We do not sell to patients.
- Purity: 98.50%
- CAS No.: 855-97-0
- Formula: C19H18O6
- Molecular Weight:342.35
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Storage:
4°C, protect from light
* In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
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Biological Activity
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Chemical Information
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Solvent & Solubility
- Purity & Documentation
- References
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Help & FAQs
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Publications Citing Use of MedChemExpress (MCE) 5,7,3',4'-Tetramethoxyflavone
MoreAll Parasite Isoforms
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Biological Activity
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Plasmodium |
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Cell Line
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Type | Value | Description | References |
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| RAW264.7 | IC50 |
3.13 μM
Compound: 3y
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Antiinflammatory activity in mouse RAW264.7 cells assessed as inhibition of LPS-stimulated PGE2 production by ELISA
Antiinflammatory activity in mouse RAW264.7 cells assessed as inhibition of LPS-stimulated PGE2 production by ELISA
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[PMID: 28408221] |
| RAW264.7 | IC50 |
32.5 μM
Compound: 3y
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Antiinflammatory activity in mouse RAW264.7 cells assessed as inhibition of LPS-stimulated NO production by ELISA
Antiinflammatory activity in mouse RAW264.7 cells assessed as inhibition of LPS-stimulated NO production by ELISA
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[PMID: 28408221] |
| RAW264.7 | IC50 |
73.94 μM
Compound: 3y
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Cytotoxicity against mouse RAW264.7 cells by MTT assay
Cytotoxicity against mouse RAW264.7 cells by MTT assay
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[PMID: 28408221] |
5,7,3',4'-Tetramethoxyflavone (5-20 μg/mL, 24 h) inhibits the expression of EP2, EP4, bcatenin and COX-2 gene in chondrocytes[1].
5,7,3',4'-Tetramethoxyflavone (5-20 μg/mL, 48 h) inhibits the protein expression of EP/cAMP/PKA signaling pathway and β-catenin signaling pathway in chondrocytes[1].
5,7,3',4'-Tetramethoxyflavone (5-20 μg/mL, 4 h) protects chondrocytes from apoptosis through regulating IRE1α[2].
5,7,3',4'-Tetramethoxyflavone (5-20 μg/mL, 4 h) reverses the expression pattern of endoplasmic reticulum (ER) stress genes due to IRE1α deficiency[2].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
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Cell Line:
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Concentration:
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Incubation Time:
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Result:
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Cell Line:Chondrocytes
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Concentration:5-20 μg/mL
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Incubation Time:4 h
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Result:Increased the protein level of Bcl-2 and XBP1s.
Decreased the expression of pro-apoptotic factors CHOP/caspase-3 and stress kinase JNK.
5,7,3',4'-Tetramethoxyflavone (5-20 mg/kg; i.v., p.o.) has the absolute bioavailability of 14.3 % in rats[3].
Pharmacokinetic Analysis in Sprague-dawley Rats Model[3]
| Route | Dose (mg/kg) | AUC (min μg/mL) | AUC/Dose (min/mL) | t1/2 (h) | Tmax (h) | Cmax (ng/mL) | Bioavailability (%) |
| i.v. | 5 | 161.49 ± 58.78 | 0.1154 ± 0.0420 | 62.85 ± 27.92 | / | / | 14.3 |
| p.o. | 50 | 231.43 ± 71.87 | 0.0165 ± 0.0051 | 273.76 ± 90.23 | 190.34 ± 24.50 | 0.79 ± 0.30 | / |
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
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Animal Model:Rat knee OA model[1]
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Dosage:25-100 mg/kg
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Administration:i.g.
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Result:Decreased the contents of IL-1b, TNF-a, and PGE2 in a dose-dependent manner.
Decreases the expression of the inflammatory cytokines in rat knee osteoarthritis (OA) synovial fluid (SF) lavages.
Inhibited chondrocytes hypertrophy and decreased the cartilage thickness.
Exhibited down regulation of b-catenin in a dose-dependent manner.
Chemical Information
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CAS No. 855-97-0
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Appearance Solid
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Molecular Weight 342.35
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Formula C19H18O6
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Color White to off-white
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SMILES
O=C1C=C(C2=CC=C(OC)C(OC)=C2)OC3=C1C(OC)=CC(OC)=C3
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Structure Classification
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Initial Source
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
4°C, protect from light
* In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)
Publications (2)
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Journal Impact Factor
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Most Recent
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Food Chem
Effects of sun drying combined with baking processes on the flavor quality of Chongqing Tuocha raw tea. [Abstract]2025 Dec 30:497:146992. PMID: 41285060 -
Food Chem
Flavonoid-mediated metabolic underpinning quality variation in red bud-sport pear mutants. [Abstract]2025 Oct 15:489:144992. PMID: 40466530
Solvent & Solubility
DMSO : 6.67 mg/mL (19.48 mM; ultrasonic and warming and heat to 60°C; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Select the appropriate dissolution method based on your experimental animal and administration route.
- For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
- To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
- The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.
Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 0.67 mg/mL (1.96 mM); Clear solution
This protocol yields a clear solution of ≥ 0.67 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (6.7 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: ≥ 0.67 mg/mL (1.96 mM); Clear solution
This protocol yields a clear solution of ≥ 0.67 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (6.7 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
Please enter the basic information of animal experiments:
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Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
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%DMSO +
Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
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%+
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+%Tween-80 + +
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%Saline +
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Working solution concentration: 0.22 mg/mL
Method for preparing stock solution: mg drug dissolved in μL DMSO. Stock solution concentration: mg/mL. * In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)
1. Take μL DMSO stock solution;
2. Add μL .
μL , mix evenly;
3. Then add μL Tween 80, mix evenly;
4. Then add μL
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
Purity & Documentation
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Data Sheet (280 KB)
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SDS (393 KB)
- English - EN (393 KB)
- Français - FR (393 KB)
- Deutsch - DE (393 KB)
- Norwegian - NO (393 KB)
- Español - ES (393 KB)
- Swedish - SV (393 KB)
- Italian - IT (393 KB)
- Korean - KR (393 KB)
- Portuguese - PT (393 KB)
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Handling Instructions (2659 KB)
References
[1]. Wu L, et al. 5,7,3',4'-Tetramethoxyflavone exhibits chondroprotective activity by targeting β-catenin signaling in vivo and in vitro. Biochem Biophys Res Commun. 2014 Sep 26;452(3):682-8. [Content Brief]
[2]. Wu L, et al. 5,7,3',4'-Tetramethoxyflavone protects chondrocytes from ER stress-induced apoptosis through regulation of the IRE1α pathway. Connect Tissue Res. 2018 Mar;59(2):157-166. [Content Brief]
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| DMSO | 1 mM | 2.9210 mL | 14.6049 mL | 29.2099 mL | 73.0247 mL |
| 5 mM | 0.5842 mL | 2.9210 mL | 5.8420 mL | 14.6049 mL | |
| 10 mM | 0.2921 mL | 1.4605 mL | 2.9210 mL | 7.3025 mL | |
| 15 mM | 0.1947 mL | 0.9737 mL | 1.9473 mL | 4.8683 mL |
5,7,3',4'-Tetramethoxyflavone Related Classifications
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