3′,4′,7-Trihydroxyflavone
Based on 1 publication(s) in Google Scholar
3′,4′,7-Trihydroxyflavone is an orally active inhibitor of OXA-48 (IC50 = 1.89 μM) and COX-1 (IC50 = 36.37 μM). 3′,4′,7-Trihydroxyflavone exhibits antioxidant and anti-inflammatory properties, inhibiting the release of inflammatory cytokines such as IL-6, IL-8, and TNF-α. 3′,4′,7-Trihydroxyflavone inhibits H2O2-induced neuronal apoptosis and ROS accumulation, and exerts anti-neuroinflammatory effects by suppressing the JNK-STAT1 pathway. 3′,4′,7-Trihydroxyflavone exhibits antimicrobial and antibiotic-modifying activities against multidrug-resistant Gram-negative enteric bacteria. 3′,4′,7-Trihydroxyflavone inhibits RANKL-induced osteoclast formation via NFATc1. 3′,4′,7-Trihydroxyflavone activates the CREB-BDNF axis and restores scopolamine (HY-N0296)-induced memory deficits in mice.
商品は「研究用試薬」です。人や動物の医療用・臨床診断用・食品用の製品ではありません。
研究用途以外に使用した場合、当社は一切の責任を負いかねます。
- 純度 : 99.04%
- CAS 番号: 2150-11-0
- 分子式: C15H10O5
- 分子量:270.24
-
保管条件:
RT, protect from light.
In solvent -80°C, 1 year , -20°C, 6 months
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
-
生物活性
-
化学情報
-
溶剤 & 溶解度
- プロトコル
- 純度とドキュメンテーション
- 参考文献
-
Help & FAQs
-
HY-14520
Tetrahydrofolic acidFolate Derivative -
HY-N0911
Rehmannioside ANeuroprotectant -
HY-113327
1,3,7-Trimethyluric acidCaffeine Metabolite -
HY-66008
N-Acetyl mesalazine5-Aminosalicylic Acid Metabolite -
HY-Z7721
(Rac)-Beraprost sodiumRacemic Isomer of Beraprost sodium -
HY-N7108
7-HydroxyflavoneAnti-inflammatory Agent -
HY-103694
NebularineAntibiotic -
HY-N2609
7,4'-DihydroxyflavoneCCL11/CBR1 Inhibitor -
HY-14905
Uridine triacetateUridine Prodrug -
HY-N7030
5,7,3',4'-TetramethoxyflavoneParasite Inhibitor -
HY-117761
MHY908PPARα/γ dual agonist -
HY-B0813
Treprostinil diethanolamineEP2/DP1/IP Agonist
MedChemExpress（MCE）の使用を引用している文献 3′,4′,7-Trihydroxyflavone
MoreBeta-lactamase アイソフォーム固有の製品をすべて表示
MoreCaspase アイソフォーム固有の製品をすべて表示
More
生物活性
製品説明
Cellular Effect
|
Cell Line
|
Type | Value | Description | References |
|---|---|---|---|---|
| CHO | IC50 |
49.3 μM
Compound: 12
|
Cytotoxicity against CHO cells by MTT assay
Cytotoxicity against CHO cells by MTT assay
|
[PMID: 19572738] |
| HEK293 | IC50 |
>40 μM
Compound: 1d
|
Inhibition of human telomerase from HEK293 cell extracts by Flash-Plate assay
Inhibition of human telomerase from HEK293 cell extracts by Flash-Plate assay
|
[PMID: 15588081] |
| HEK293 | IC50 |
>40 μM
Compound: Fig 20, R2C1
|
Inhibition of telomerase extracted from HEK293 cells using 5'-bAATCCGTCGAGCAGAGTT as primer assessed as polymerization of telomeric repeats to the end of the primers after 2 hrs by Flash-Plate assay
Inhibition of telomerase extracted from HEK293 cells using 5'-bAATCCGTCGAGCAGAGTT as primer assessed as polymerization of telomeric repeats to the end of the primers after 2 hrs by Flash-Plate assay
|
10.1039/C0MD00241K |
| Neutrophil | IC50 |
13.5 μM
Compound: 3d
|
Inhibition of oxidative burst in PMA-stimulated human neutrophils assessed as inhibition of ROS-induced luminol oxidation incubated for 5 mins prior to PMA challenge by chemiluminescence assay
Inhibition of oxidative burst in PMA-stimulated human neutrophils assessed as inhibition of ROS-induced luminol oxidation incubated for 5 mins prior to PMA challenge by chemiluminescence assay
|
[PMID: 23871908] |
| Neutrophil | IC50 |
14.7 μM
Compound: 3d
|
Inhibition of oxidative burst in PMA-stimulated human neutrophils assessed as inhibition of superoxide anion radical-induced lucigenin oxidation incubated for 5 mins prior to PMA challenge by chemiluminescence assay
Inhibition of oxidative burst in PMA-stimulated human neutrophils assessed as inhibition of superoxide anion radical-induced lucigenin oxidation incubated for 5 mins prior to PMA challenge by chemiluminescence assay
|
[PMID: 23871908] |
| Neutrophil | IC50 |
3.7 μM
Compound: 3d
|
Inhibition of oxidative burst in PMA-stimulated human neutrophils assessed as inhibition of H2O2-induced oxidation of amplex red incubated for 5 mins prior to PMA challenge by fluorescence assay
Inhibition of oxidative burst in PMA-stimulated human neutrophils assessed as inhibition of H2O2-induced oxidation of amplex red incubated for 5 mins prior to PMA challenge by fluorescence assay
|
[PMID: 23871908] |
| Neutrophil | IC50 |
5.2 μM
Compound: 3d
|
Inhibition of oxidative burst in PMA-stimulated human neutrophils assessed as inhibition of HOCl-induced oxidation of APF after 6 mins by fluorescence assay
Inhibition of oxidative burst in PMA-stimulated human neutrophils assessed as inhibition of HOCl-induced oxidation of APF after 6 mins by fluorescence assay
|
[PMID: 23871908] |
| Peritoneal macrophage | IC50 |
26 μM
Compound: kp15
|
Inhibition of LPS-stimulated nitric oxide production in ddy mouse peritoneal macrophages measured after 20 hrs by Greiss method
Inhibition of LPS-stimulated nitric oxide production in ddy mouse peritoneal macrophages measured after 20 hrs by Greiss method
|
[PMID: 27955927] |
体外実験
3′,4′,7-Trihydroxyflavone (0.1-20 μM, 24 h) enhances the cell viability and survival against Scopolamine (HY-N0296)-induced damage in SH-SY5Y cells[1].
3′,4′,7-Trihydroxyflavone (0.1-100 µM) reduces the secretion of IL-6 and IL-8 in TNFα-stimulated HaCaT cells, with IC50s of 17.8, 126.2 µg/mL, with maximum inhibition rates of 74.4, 40.2 %, reduces HaCaT cell viability to 74%[2].
3′,4′,7-Trihydroxyflavone (50 µM, 64 μg/mL) inhibits OXA-48 activity by 80% and exhibits antimicrobial activity against BW25113 ∆acrA∆bamB (OXA-48) in combination with β-lactam antibiotics[3].
3′,4′,7-Trihydroxyflavone (1-20 μM, 30 min) prevents H2O2-induced cell death and lactate dehydrogenase (LDH) release in SH-SY5Y and hippocampal neuronal cells[4].
3′,4′,7-Trihydroxyflavone (1-20 µM, 30 min) inhibits H2O2-induced apoptotic features of SH-SY5Y cells, reverses induced nuclear necrosis, and condenses cell shrinkage[4].
3′,4′,7-Trihydroxyflavone (0.5-20 µM, 30 min) inhibits H2O2-induced ROS accumulation, SOD, CAT, and GSH reduction, and activation of F-κB p65 translocation from the cytosol to the nucleus in SH-SY5Y cells[4].
3′,4′,7-Trihydroxyflavone (1-20 µM, 30 min) inhibits H2O2-induced phosphorylation of JNK, p38, ERK 1/2 MAPKs and PI3K/Akt levels, upregulation of Bax, caspase-3, caspase-9 and PARP levels, downregulation of Bcl-2 and Bcl-xL levels, release of cytochrome c and loss of MMP in SH-SY5Y cells[4].
3′,4′,7-Trihydroxyflavone shows antibacterial activity against 12 Gram-negative bacteria E. coli, E. aerogenes, K. pneumonia, with MIC values ranging from 4 to 256 μg/mL[5].
3′,4′,7-Trihydroxyflavone improves the activity of Tetracycline (HY-A0107) and Erythromycin (HY-B0220) on 80% of the tested bacteria, with FIC values ranging from 0.5 to < 0.062[5].
3′,4′,7-Trihydroxyflavone (0.1-100 μM) has no effect at 0.1–30 µM, but decreases cell viability at 100 µM for MG6 cells[6].
3′,4′,7-Trihydroxyflavone (0.1-10 μM, 6-48 h) reduces NO, TNF-α, and iNOS levels, inhibits STAT1 phosphorylation and total STAT1 expression in LPS (HY-D1056)-induced MG6 cells[6].
3′,4′,7-Trihydroxyflavone (0.1-10 μM, 0.5 h) inhibits JNK phosphorylation but not p38 or ERK phosphorylation in LPS-induced MG6 cells[6].
3′,4′,7-Trihydroxyflavone (0.1-10 μM, 24-48 h) inhibits NO release, iNOS expression, STAT1 phosphorylation in IFN-γ-induced MG6 cells[6].
3′,4′,7-Trihydroxyflavone (0-5 μg/mL, 1-7 days) inhibits RANKL-induced osteoclast formation and bone resorption in BMMs[7].
3′,4′,7-Trihydroxyflavone (5 μg/mL, 7 days) suppresses RANKL-induced NFATc1 expression via Blimp-1 and p38 MAPK pathway in BMMs[7].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
-
Cell Line:SH-SY5Y cells
-
Concentration:0.1, 1, 5, 10, and 20 μM
-
Incubation Time:24 h
-
Result:Enhanced the cell viability and survival against Scopolamine (HY-N0296)-induced damage.
-
Cell Line:SH-SY5Y cells
-
Concentration:0.1, 1, 5, 10, and 20 μM
-
Incubation Time:30 min
-
Result:Inhibited apoptosis, reversed H2O2-induced nuclear necrosis and condensation, and cell shrinkage.
-
Cell Line:SH-SY5Y cells
-
Concentration:0.5, 1, 5, 10, and 20 μM
-
Incubation Time:30 min
-
Result:Reduced ROS, SOD, CAT and GSH fluorescence intensity.
Increased the fluorescence intensity of MMP and rhodamine 123, eliminated cytochrome c diffusion caused by H2O2.
-
Cell Line:SH-SY5Y cells
-
Concentration:1, 5, 10, and 20 μM
-
Incubation Time:30 min
-
Result:Reduced the phosphorylation of Akt, JNK, p38 MAPK, and Akt to 40.64%, 111%, 105%, and 95% of the control value, respectively.
Inhibited the upregulation or downregulation of Bax and Bcl-xL to 118% and 62% of control values, respectively at 1 µM, inhibited the upregulation or downregulation of Bax, Bcl-2, and Bcl-xL to 111%, 87%, and 82% of control values, respectively, at 5 µM, inhibited the upregulation or downregulation of Bax, Bcl-2, and Bcl-xL to 102%, 98%, and 93% of control values, respectively at 20 µM.
Reduced the release of cytochrome c from mitochondria to the cytosol.
Significantly inhibited the increase in cleaved PARP, cleaved caspase-3 and cleaved caspase-9.
-
Cell Line:LPS-induced MG6 cells
-
Concentration:0.1, 1, and 10 μM
-
Incubation Time:0.5, 6, 24 h
-
Result:Reduced iNOS levels.
Inhibited STAT1 phosphorylation and total STAT1 expression.
Inhibited JNK phosphorylation but not p38 or ERK phosphorylation.
-
Cell Line:IFN-γ-induced MG6 cells
-
Concentration:0.1, 1, and 10 μM
-
Incubation Time:24 h
-
Result:Inhibited NO release and increased iNOS expression.
Inhibited STAT1 phosphorylation but did not inhibit the increase in total STAT1 levels.
-
Cell Line:BMMs
-
Concentration:5 μg/mL with RANKL (100 ng/mL) and M-CSF (30 ng/mL)
-
Incubation Time:7 days
-
Result:Suppressed the mRNA expression levels of CTR and cathepsin K.
Suppressed the expression of DC-STAMP and ATP6v0d2 induced by RANKL. Decreased the mRNA level of Blimp1.
-
Cell Line:BMMs
-
Concentration:5 μg/mL with RANKL (200 ng/mL)
-
Incubation Time:30 min
-
Result:Abolished RANKL-induced NFATc1 expression, but not c-Fos protein expression.
Suppressed phosphorylation of p38.
体内実験
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
-
Animal Model:Scopolamine-induced cognitive deficits mice (male C57BL/6J, 8 weeks old) model[1]
-
Dosage:10/50 mg/kg
-
Administration:p.o., once a day, 3 days/three times per week, 28 days
-
Result:Improved cognitive abilities at 50 mg/kg.
Had no acute cognitive improvement after three days.
Restored ACh and ChAT levels and reduced AChE activity, increased BDNF mRNA and protein levels, and restored pCREB levels at 50 mg/kg.
Increased the number of neurons and restored astrocyte levels (GFAP marker).
-
Animal Model:Scopolamine-induced cognitive deficits mice (male C57BL/6J, 8 weeks old) model[1]
-
Dosage:10 ng
-
Administration:ICV, twice a week, 28 days
-
Result:Improved spontaneous change, novel object recognition index and passive avoidance, acetylcholine activity, and restored acetylcholinesterase and choline acetyltransferase activities.
Induced the enhancement of the CREB-BDNF signaling pathway and increased LTP.
- Research Protocol for Infectious Diseases
- Detection of Apoptosis
- TUNEL staining for apoptotic DNA fragmentation
- LPS-Induced Endotoxemia/Systemic Inflammation
- Annexin V plus membrane-impermeant dye apoptosis staining
- ROS/oxidative-stress fluorescent staining
- Apoptosis Solutions
- Research Protocol for Inflammation-related Diseases
- Gram Staining of Tissue Sections
- Osteoclast differentiation from monocyte/macrophage precursors
化学情報
-
CAS 番号 2150-11-0
-
性状 Solid
-
分子量 270.24
-
分子式 C15H10O5
-
Color Off-white to light yellow
-
SMILES
O=C1C2=CC=C(O)C=C2OC(C3=CC(O)=C(O)C=C3)=C1
-
Structure Classification
-
Initial Source
-
輸送条件
Room temperature in continental US; may vary elsewhere.
-
保管条件
RT, protect from light
In solvent -80°C 1 year -20°C 6 months
Publications (1)
-
Journal Impact Factor
-
Most Recent
-
Spectrochim Acta A Mol Biomol Spectrosc
Communication of bifunctional G-quadruplex chaperone with aptamer for highly selective adenosine signaling by an ESIPT fluorogen. [Abstract]2026 Jun 5:354:127637. PMID: 41762796
溶剤 & 溶解度
体外:
DMSO : 2.5 mg/mL (9.25 mM; ultrasonic and warming and heat to 60°C; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 1 year; -20°C, 6 months. When stored at -80°C, please use it within 1 year. When stored at -20°C, please use it within 6 months.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 1 year; -20°C, 6 months. When stored at -80°C, please use it within 1 year. When stored at -20°C, please use it within 6 months.
濃度 (開始) × 体積 (開始) = 濃度 (終了) × 体積 (終了)
純度とドキュメンテーション
-
データシート (293 KB)
-
SDS (394 KB)
- English - EN (394 KB)
- Français - FR (394 KB)
- Deutsch - DE (394 KB)
- Norwegian - NO (394 KB)
- Español - ES (394 KB)
- Swedish - SV (394 KB)
- Italian - IT (394 KB)
- Korean - KR (394 KB)
- Portuguese - PT (394 KB)
-
取扱説明書 (2659 KB)
参考文献
[1]. Kim SS, et al. 3',4',7-trihydroxyflavone activates the CREB-BDNF axis and restores scopolamine-induced memory deficit in mice. Eur J Pharmacol. 2025 Jul 15;999:177645 [Content Brief]
[2]. Codo Toafode NM, et al. Anti-Inflammatory Potential of Phenolic Compounds Isolated From Entada africana Guill. & Perr. Used in the Republic of Benin. Front Pharmacol. 2022 Jun 30;13:931240. [Content Brief]
[3]. Zhang Y, et al. Discovery of Quercetin and Its Analogs as Potent OXA-48 Beta-Lactamase Inhibitors. Front Pharmacol. 2022 Jun 22;13:926104. [Content Brief]
[4]. Kwon SH, et al. 3',4',7-Trihydroxyflavone prevents apoptotic cell death in neuronal cells from hydrogen peroxide-induced oxidative stress. Food Chem Toxicol. 2015 Jun;80:41-51. [Content Brief]
[5]. Dzotam JK, et al. In vitro antibacterial and antibiotic modifying activity of crude extract, fractions and 3',4',7-trihydroxyflavone from Myristica fragrans Houtt against MDR Gram-negative enteric bacteria. BMC Complement Altern Med. 2018 Jan 15;18(1):15. [Content Brief]
[6]. Akaishi T, et al. 3',4',7-Trihydroxyflavone Downregulates NO Production in LPS- or IFN-γ-Activated MG6 Microglial Cells by Attenuating the JNK-STAT1 Pathway. Biol Pharm Bull. 2022;45(3):301-308. [Content Brief]
[7]. Kang JH, et al. 3'4'7-Trihydroxyflavone inhibits RANKL-induced osteoclast formation via NFATc1. Pharmazie. 2015 Oct;70(10):661-7. [Content Brief]
[8]. Selvam C, et al. Cyclooxygenase inhibitory flavonoids from the stem bark of Semecarpus anacardium Linn. Phytother Res. 2004 Jul;18(7):582-4. [Content Brief]
[9]. Jung MJ, et al. Antioxidant activity from the stem bark of Albizzia julibrissin. Arch Pharm Res. 2003 Jun;26(6):458-62. [Content Brief]
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 1 year; -20°C, 6 months. When stored at -80°C, please use it within 1 year. When stored at -20°C, please use it within 6 months.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| DMSO | 1 mM | 3.7004 mL | 18.5021 mL | 37.0041 mL | 92.5104 mL |
| 5 mM | 0.7401 mL | 3.7004 mL | 7.4008 mL | 18.5021 mL |
3′,4′,7-Trihydroxyflavone 関連分類
HY-N2736 関連分類
Powered by Bioz
Keywords
- 3′,4′,7-Trihydroxyflavone
- 2150-11-0
- Beta-lactamase
- COX
- Interleukin Related
- Bacterial
- JNK
- ERK
- p38 MAPK
- STAT
- Apoptosis
- NO Synthase
- Nuclear Factor of activated T Cells (NFAT)
- Lactate Dehydrogenase
- Reactive Oxygen Species (ROS)
- SOD
- Akt
- Caspase
- Bcl-2 Family
- flavonoid aglycone
- cognitive deficits mice model
- SH-SY5Y cells
- HaCaT cell
- MG6 cells
- BMMs
- Inhibitor
- inhibitor
- inhibit